Using immunofluorescence microscopy, the cortical microtubular net which is regularly present in cells of young, growing tissue is shown to be absent, or largely reduced, in mature mesophyll cells of Nicotiana plumbaginifolia Viv., N. tabacum L., Petunia hybrida Hort. and Brassica napus L. The onset of division in protoplasts isolated from these fully differentiated tissues is preceded by a period of dedifferentiation. One of the early events during dedifferentiation, as shown for N. plumbaginifolia, is the re-establishment of a net of cortical microtubules, prior to spindle formation. These findings indicate that the presence of the cortical microtubular lattice is a prerequisite for protoplast division. Cell-wall regeneration, which also must precede division, occurs simultaneously with the formation of the lattice. However, the cortical microtubules seem to not exert any influence on the orientation of the microfibrils.
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Evans, D.A., Bravo, J.E. (1983) Protoplast isolation and culture. In: Handbook of plant cell culture, vol. 1, pp. 124–176, Evans, D.A., Sharp, W.R., Ammirato, P.V., Yamada, Y., eds. Macmillan, New York
Fowke, L.C., Bech-Hansen, C.W., Gamborg, O.L. (1974) Electron microscopic observation of cell regeneration from cultured protoplasts of Ammi visnaga. Protoplasma 79, 235–248
Fowke, L.C., Simmonds, D., van der Valk, P., Setterfield, G. (1984) Immunofluorescence techniques for studies of plant microtubules. In: Cell culture and somatic cell genetics of plants, vol. 1, pp. 785–794, Vasil, I.K., ed. Academic Press, Orlando
Gigot, C., Kopp, M., Schmitt, C., Milne, R.G. (1975) Subcellular changes during isolation and culture of tobacco mesophyll protoplasts. Protoplasma 84, 31–41
Gunning, B.E.S. (1982) The cytokinetic apparatus: Its development and spatial regulation. In: The cytoskeleton in plant growth and development, pp. 230–292, Lloyd, C.W., ed. Academic Press, London New York
Hahne, G., Herth, W., Hoffmann, F. (1983) Wall formation and cell division in flurescence-labelled plant protoplasts. Protoplasma 115, 217–221
Hahne, G., Hoffmann, F. (1984) Dimethyl sulfoxide can initiate division of arrested callus protoplasts by promoting cortical microtubule assembly. Proc. Natl. Acad. Sci. USA 81, 5449–5453
Hensel, W. (1984) Microtubules in statocytes from roots of cress (Lepidium sativum L.). Protoplasma 119, 121–134
Lloyd, C.W., Barlow, P.W. (1982) The co-ordination of cell division and elongation: the role of the cytoskeleton. In: The cytoskeleton in plant growth and development, pp. 203–228, Lloyd, C.W., ed. Academic Press, London New York
Lloyd, C.W., Slabas, A.R., Powell, A.J., MacDonald, G., Badley, R.A. (1979) Cytoplasmic microtubules of higher plant cells visualized with anti-tubulin antibodies. Nature 279, 239–241
Lloyd, C.W., Slabas, A.R., Powell, A.J., Lowe, S.B. (1980) Microtubules, protoplasts and plant cell shape. An immunofluorescent study. Planta 147, 500–506
Maeda, H., Ishida, N. (1967) Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener. J. Biochem. 62, 276–278
Melchers, G. (1977) Kombination somatischer und konventioneller Genetik für die Pflanzenzüchtung. Naturwissenschaften 64, 184–194
Morejohn, L.C., Bureau, T.A., Tocci, L., Fosket, D.E. (1984) Tubulins from different higher plant species are immunologically nonidentical and bind colchicine differentially. Proc. Natl. Acad. Sci. USA 81, 1440–1444
Robinson, D.G., Quader, H. (1982) The microtubule-microfibril syndrome. In: The cytoskeleton in plant growth and development, pp. 109–126, Lloyd, C.W., ed. Academic Press, London New York
Schilde-Rentschler, L. (1977)_Role of the cell wall in the ability of tobacco protoplasts to form callus. Planta 135, 177–181
Takebe, I., Otsuki, Y. (1973) Fine structure of isolated mesophyll protoplasts of tobacco. Planta 113, 21–27
Thomas, E., Hoffmann, F., Potrykus, I., Wenzel, G. (1976) Protoplast regeneration and stem embryogenesis of haploid androgenetic rape. Mol. Gen. Genet. 145, 235–247
Wick, S.M., Duniec, J. (1983) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. I. Preprophase band development and concomitant appearance of nuclear envelope-associated tubulin. J. Cell Biol. 97, 235–243
Wick, S.M., Duniec, J. (1984) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. II. Transition between the preprophase band and the mitotic spindle. Protoplasma 122, 45–55
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Hahne, G., Hoffmann, F. Cortical microtubular lattices: Absent from mature mesophyll and necessary for cell division?. Planta 166, 309–313 (1985). https://doi.org/10.1007/BF00401166
- Cell dedifferentiation
- Cell division
- Microtubule, cortical
- Nicotiana (microtubules)