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Cortical microtubular lattices: Absent from mature mesophyll and necessary for cell division?

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Abstract

Using immunofluorescence microscopy, the cortical microtubular net which is regularly present in cells of young, growing tissue is shown to be absent, or largely reduced, in mature mesophyll cells of Nicotiana plumbaginifolia Viv., N. tabacum L., Petunia hybrida Hort. and Brassica napus L. The onset of division in protoplasts isolated from these fully differentiated tissues is preceded by a period of dedifferentiation. One of the early events during dedifferentiation, as shown for N. plumbaginifolia, is the re-establishment of a net of cortical microtubules, prior to spindle formation. These findings indicate that the presence of the cortical microtubular lattice is a prerequisite for protoplast division. Cell-wall regeneration, which also must precede division, occurs simultaneously with the formation of the lattice. However, the cortical microtubules seem to not exert any influence on the orientation of the microfibrils.

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Hahne, G., Hoffmann, F. Cortical microtubular lattices: Absent from mature mesophyll and necessary for cell division?. Planta 166, 309–313 (1985). https://doi.org/10.1007/BF00401166

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Key words

  • Cell dedifferentiation
  • Cell division
  • Microtubule, cortical
  • Nicotiana (microtubules)
  • Protoplast