Examination of islets in the pancreas biopsy specimens from newly diagnosed Type 1 (insulin-dependent) diabetic patients
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We attempted to examine the immunopathological change of the pancreatic islets of newly diagnosed Type 1 (insulin-dependent) diabetic patients and thereby to obtain useful information for the therapy of the patients. For this purpose, pancreas biopsy under laparoscopy was performed 2–4 months after the onset of Type 1 diabetes in seven newly diagnosed patients. All biopsies were performed safely without any complications. Immunohistochemical examination of the biopsy specimens revealed a marked decrease of insulin-containing cells, preservation of glucagon-containing cells, and various degrees of expression of MHC class I and class II antigens in islet cells and in endothelial cells within and around the islets. Signs of active autoimmune phenomena, e. g. lymphocytic infiltration or immunoglobulin deposition in islets, were not detected in any of these patients by light microscopical evaluation. We conclude that pancreas biopsy under laparoscopy has shown various immunological changes in the islets of newly diagnosed Type 1 diabetic patients. Pancreas biopsy, however, may not be suitable under the present protocol for the selection of patients for immunotherapy because of problems including sampling errors.
Key wordsType 1 (insulin-dependent) diabetes mellitus pathology pathogenesis diagnosis pancreas biopsy laparoscopy immunohistochemistry MHC class I antigen MHC class II antigen immunotherapy
- 4.Junker K, Egeberg J, Kromann H, Nerup J (1977) An autopsy study of the islets of Langerhans in acute-onset juvenile diabetes mellitus. Acta Pathol Microbiol Scand Sect A 85: 699–706Google Scholar
- 5.Doniach I, Morgan AG (1973) Islets of Langerhans in juvenile diabetes mellitus. Clin Endocrinol 2: 233–248Google Scholar
- 8.Meyer-Burg J (1972) The inspection, palpation and biopsy for the pancreas. Endoscopy 4: 99–101Google Scholar
- 11.Foulis AK, Farquharson MA, Hardman R (1987) Aberrant expression of Class II major histocompatibility complex molecules by B cells and hyperexpression of Class I major histocompatibility complex molecules by insulin containing islets in Type 1 (insulin-dependent) diabetes mellitus. Diabetologia 30: 333–343PubMedGoogle Scholar