A micro-radioisotopic antiglobulin assay has been employed to determine the binding of monoclonal antibodies to subcellular membranes adsorbed to the wells of Terasaki Microtest Plates. A panel of 15 monoclonal antibodies was tested against 22 membrane preparations isolated from normal and malignant colo-rectal, breast and lung tissues. The assay provided an objective evaluation of antibody reactivity permitting the expression of major antigens in samples from individual surgical specimens to be defined.
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Brown A, Feizi T, Gooi HC, Embleton MJ, Picard JK, Baldwin RW (1983) A monoclonal antibody against human colonic adenoma recognizes difucosylated Type-2 blood group chains. Biosci Rep 3:163–170
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Price, M.R., Edwards, S. & Baldwin, R.W. Application of a subcellular membrane-antibody binding assay for the analysis of antigen expression in human tumours. J Cancer Res Clin Oncol 111, 169–172 (1986). https://doi.org/10.1007/BF00400759
- Membrane-antibody binding
- Human tumours