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Application of a subcellular membrane-antibody binding assay for the analysis of antigen expression in human tumours

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Summary

A micro-radioisotopic antiglobulin assay has been employed to determine the binding of monoclonal antibodies to subcellular membranes adsorbed to the wells of Terasaki Microtest Plates. A panel of 15 monoclonal antibodies was tested against 22 membrane preparations isolated from normal and malignant colo-rectal, breast and lung tissues. The assay provided an objective evaluation of antibody reactivity permitting the expression of major antigens in samples from individual surgical specimens to be defined.

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References

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Correspondence to M. R. Price.

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Price, M.R., Edwards, S. & Baldwin, R.W. Application of a subcellular membrane-antibody binding assay for the analysis of antigen expression in human tumours. J Cancer Res Clin Oncol 111, 169–172 (1986). https://doi.org/10.1007/BF00400759

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Key words

  • Membrane-antibody binding
  • Antigen
  • Human tumours