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Diabetologia

, Volume 36, Issue 2, pp 113–116 | Cite as

Peroxovanadate but not vanadate exerts insulin-like effects in human adipocytes

  • P. Lönnroth
  • J. W. Eriksson
  • B. I. Posner
  • U. Smith
Originals

Summary

Vanadate and peroxovanadate were recently reported to exert maximal or even supramaximal (peroxovanadate) insulin-like effects in rat adipocytes. To evaluate the response in human cells, isolated human adipocytes were exposed to insulin or various concentrations of vanadate (0–10 mmol/l) or peroxovanadate (0–5 mmol/l). Neither vanadate nor peroxovanadate affected 125I-insulin binding and insulin sensitivity. Vanadate exerted no apparent effect on 14C-U-glucose uptake, whereas 0.1 mmol/l peroxovanadate exerted a full insulin-like response (p<0.001). No additive response was observed by combining either vanadate or peroxovanadate with insulin. Peroxovanadate at 0.1 mmol/l was as effective as insulin in inhibiting isoproterenol-stimulated lipolysis. Neither peroxovanadate nor insulin-inhibited lipolysis stimulated by N6-monobutyryl-cAMP, an analogue which is not hydrolysed by the cAMP-phosphodiesterase. It is concluded that peroxovanadate, but not vanadate, elicits a full insulin-like response in human adipocytes.

Key words

Vanadate peroxovanadate adipose cells lipolysis glucose transport 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • P. Lönnroth
    • 1
    • 3
  • J. W. Eriksson
    • 1
  • B. I. Posner
    • 2
  • U. Smith
    • 1
  1. 1.Department of MedicineUniversity of GöteborgGöteborgSweden
  2. 2.Department of MedicineMcGill UniversityMontrealCanada
  3. 3.Department of MedicineSahlgren's Hospital45 GöteborgSweden

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