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Pancreatic Reg/pancreatic stone protein (PSP) gene expression does not correlate with beta-cell growth and regeneration in rats

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The Reg/pancreatic stone protein (PSP) gene is postulated to be an important regulator of pancreatic beta-cell growth. To investigate this hypothesis, we analysed the expression of the Reg/PSP gene following a 90% pancreatectomy and after chronic glucose infusion, two well-defined models of pancreatic beta-cell growth. There was a rapid induction of the Reg/PSP gene in the remnant pancreas after a 90% pancreatectomy in rats during the period of marked growth of the exocrine and islet tissue. However, a similar rapid, but smaller, induction of the Reg/PSP gene was observed in sham-operated rats and in non-surgical control rats in which there was no enhanced pancreatic growth. Furthermore, there was no pancreatic Reg/PSP gene induction in a model of selective beta-cell growth, the chronic glucose-infused rat. Thus, it is unlikely that Reg/PSP is a beta-cell specific growth factor, even though the function of this important pancreatic gene is still unknown.



Partial pancreatectomy


pancreatic stone protein


  1. 1.

    Terazono K, Yamamoto H, Takasawa S et al. (1988) A novel gene activated in regenerating islets. J Biol Chem 263: 2111–2114

  2. 2.

    Unno M, Yonekura H, Nakagawara K et al. (1993) Structure, chromosome localization, and expression of mouse reg genes, reg I and reg II. J Biol Chem 268: 15974–15982

  3. 3.

    De Caro AM, Adrich Z, Fournet B et al. (1989) N-terminal sequence extension in the glycosylated forms of human pancreatic stone protein. The 5 oxoproline N-terminal chain is 0-glycosylated on the 5th amino acid residue. Biochim Biophys Acta 994: 281–284

  4. 4.

    Watanabe T, Yonekura H, Terazono K, Yamamoto H, Okamoto H (1990) Complete nucleotide sequence of human reg gene and its expression in normal and tumoral tissues. J Biol Chem 265: 7432–7439

  5. 5.

    Gross J, Carlson RI, Brauer AW, Margolies MN, Warshaw AL, Wands JR (1985) Isolation, characterization and distribution of an unusual pancreatic human secretory protein. J Clin Invest 76: 2115:2126

  6. 6.

    Giorgi D, Bernard J-P, Rouquier S, Iovanna J, Sarles H, Dagorn J-C (1989) Secretory pancreatic stone protein messenger RNA. J Clin Invest 84: 100–106

  7. 7.

    Bernard JP, Adrich Z, Montalo G et al. (1991) Immunoreactive forms of pancreatic stone protein in six mammalian species. Pancreas 6: 162–167

  8. 8.

    Miyaura C, Chen L, Appel M et al. (1991) Expression of reg/PSP, a pancreatic exocrine gene: relationship to changes in islet Β-cell mass. Mol Endocrinol 5: 226–234

  9. 9.

    Francis PJ, Southgate JL, Wilkin TJ, Bone AJ (1992) Expression of an islet regenerating (reg) gene in isolated rat islets: effects of nutrient and non-nutrient growth factors. Diabetologia 35: 238–242

  10. 10.

    Yamadera K, Moriyama T, Makino I (1990) Identification of immunoreactive pancreatic stone protein in pancreatic stone, pancreatic tissue, and pancreatic juice. Pancreas 5: 255–260

  11. 11.

    Shimosegawa T, Koizumi M, Moriizumi S, Okamoto H, Toyota T (1990) Immunohistochemical demonstration of reg protein in the normal rat and human pancreas. Pancreas 5: 731 (Abstract)

  12. 12.

    Terazano K, Uchiyama Y, Ide M et al. (1990) Expression of reg protein in rat regenerating islets and its co-localization with insulin the beta-cell secretory granules. Diabetologia 33: 250–252

  13. 13.

    Banister SH, Cole DR, Sarsfield P et al. (1992) Reg protein localization in pancreatic tissue from patients with nesidioblastosis and insulinoma. Diabetes 41: 161 (Abstract)

  14. 14.

    Senegas-Balas FO, Figarella CG, Amouric MA, Guy-Crotte OM, Bertrand CA, Balas DC (1991) Immunocytochemical demonstration of a pancreatic secretory protein of unknown function in the human duodenum. J Histochem Cytochem 39: 915–919

  15. 15.

    de la Monte SM, Ozturk M, Wands JR (1990) Enhanced expression of an exocrine pancreatic protein in Alzheimer's disease and the developing human brain. J Clin Invest 86: 1004–1013

  16. 16.

    Bonner-Weir S, Trent DF, Weir GC (1983) Partial pancreatectory in the rat and subsequent defect in glucose-infused insulin release. J Clin Invest 71: 1544–1553

  17. 17.

    Brockenbrough JS, Weir GC, Bonner-Weir S (1988) Discordance of exocrine and endocrine growth following 90% partial pancreatectomy in the rat. Diabetes 37: 232–236

  18. 18.

    Leahy JL, Cooper HE, Deal DA, Weir GC (1986) Chronic hyperglycemia is associated with impaired glucose influence on insulin secretion. A study in normal rats using chronic in vivo glucose infusions. J Clin Invest 77: 908–915

  19. 19.

    Bonner-Weir S, Deery D, Leahy JL, Weir GC (1989) Compensatory growth of pancreatic B-cells in adult rats after short-term glucose infusion. Diabetes 38: 49–53

  20. 20.

    Bonner-Weir S, Baxter LA, Schuppin GT, Smith FE (1993) A second pathway for regeneration of adult exocrine and endocrine pancreas, a possible recapitulation of embryonic development. Diabetes 42: 1715–1720

  21. 21.

    Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159

  22. 22.

    Rosen KM, Lamperti ED, Villa-Komaroff L (1990) Optimizing the Northern blot procedure. Biotechniques 8: 398–403

  23. 23.

    Osborn L, Rosenberg MP, Kelly SA, Meisler MH (1987) Tissue-specific and insulin-dependent expression of a pancreatic amylase gene in transgenic mice. Mol Cell Biol 7: 326–334

  24. 24.

    Newgard CB, Hughes S, Chen L, Okamoto H, Milburn JL (1989) The reg gene is preferentially expressed in the exocrine pancreas during islet regeneration. Diabetes 38: 49 (Abstract)

  25. 25.

    De Vroede MA, In't Veld PA, Pipeleers DG (1990) Deoxyribonucleic acid synthesis in cultured adult rat pancreatic B cells. Endocrinology 127: 1510–1516

  26. 26.

    Yoon H-I, Lee P-C (1992) Autologous regulation of pancreatic glucocorticoid receptors in suckling rats. Pancreas 7: 226–232

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Correspondence to Dr. F. E. Smith.

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Smith, F.E., Bonner-Weir, S., Leahy, J.L. et al. Pancreatic Reg/pancreatic stone protein (PSP) gene expression does not correlate with beta-cell growth and regeneration in rats. Diabetologia 37, 994–999 (1994). https://doi.org/10.1007/BF00400462

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Key words

  • Pancreas
  • growth factors
  • gene expression
  • beta cells