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Isolation of chloramphenicol-resistant mutants of Kluyveromyces lactis and characterization by mitotic segregation analysis of fused hybrids

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Abstract

Chloramphenicol-resistant mutants of Kluyveromyces lactis were isolated following manganese mutagenesis. Mutants were characterized biochemically in order to determine the effect of CAP on respiration. The genetic basis of the resistance was determined using mitotic segregation analysis of fused hybrids where the transmission of mitochondrial genes from a parent in each parasexual cross was impaired by treatment with ethidium bromide.

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References

  1. Allmark, B. M., Danks, S. M. and Whittaker, P. A. 1977. Isolation and characterization of respiratory-deficient mutants of Kluyveromyces lactis, a petite-negative yeast. — Biochem. Soc. Trans. 5: 1498–1500.

  2. Allmark, B. M., Morgan, A. J. and Whittaker, P. A. 1978. The use of protoplast fusion in demonstrating chromosomal and mitochondrial inheritance of respiratory-deficiency in Kluyveromyces lactis, a petite-negative yeast. — Mol. Gen. Genet. 159: 297–299.

  3. Birky, C. W., Demko, C. A., Perlman, P. S. and Strausberg, R. 1978. Uniparental inheritance of mitochondrial genes in yeast: dependence on input bias of mitochondrial DNA preliminary investigations of the mechanism. — Genetics 89: 615–651.

  4. Brunner, A., De Cobos, A. T. and Griffiths, D. E. 1977. The isolation and genetic characterization of extrachromosomal chloramphenicol and oligomycin-resistant mutants from the petite-negative yeast Kluyveromyces lactis. — Mol. Gen. Genet. 152: 183–191.

  5. Bulder, C. J. E. A. 1963. On respiratory deficiency in yeasts. — Ph. D. Thesis, Technische Hogeschool, Delft.

  6. Coen, D., Deutsch, J., Netter, P., Petrochilo, E. and Slonimski, P. P. 1970. Mitoch genetics I. Methodology and phenomenology. — Symp. Soc. Exp. Biol. 24: 449–496.

  7. Ferenczy, L. 1981. Microbial protoplast fusion. p. 1–34. In S. W. Glover and D. A. Hopwood (eds), Genetics as a Tool in Microbiology. — Cambridge University Press, Cambridge.

  8. Ferenczy, L. and Maráz, A. 1977. Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae. — Nature (London) 268: 524–525.

  9. Gunge, N and Sakaguchi, K. 1979. Fusion of mitochondria with protoplasts in Saccharomyces cerevisiae. — Mol. Gen. Genet. 170: 243–247.

  10. Hamburger, K. and Kramhøft, B. 1980. The effect of chloramphenicol on respiration, fermentation and growth in Schizosaccharomyces pombe. — J. Gen. Microbiol. 120: 279–282.

  11. Heritage, J. and Whittaker, P. A. 1977. Isolation of metabolically active petite mutants of Kluyveromyces lactis, a petite-negative yeast. — Mol. Gen. Genet. 156: 93–98.

  12. Herman, A. and Roman, H. 1966. Allele specific determinants of homothallism in Saccharomyces lactis. — Genetics 53: 727–740.

  13. Herman, A. I. and Griffin, P. S. 1968. Respiratory-deficient mutants in Saccharomyces lactis. —J. Bacteriol. 96: 457–461.

  14. Hinnen, A., Hicks, J. B. and Fink, G. R. 1978. Transformation of yeast. — Proc. Natl Acad. Sci. USA 75: 1929–1933.

  15. Lückemann, G., Sipiczki, M. and Wolf, K. 1979. Transmission, segregation, and recombination of mitochondrial genomes in zygote clones and protoplast fusion clones of yeast. — Mol. Gen. Genet. 177: 185–187.

  16. Luha, A. A., Sarcoe, L. E. and Whittaker, P. A. 1971. Biosynthesis of yeast mitochondria. Drug effects on the petite negative yeast Kluyveromyces lactis. — Biochem. Biophys. Res. Commun. 44: 396–402.

  17. Michaelis, G. and Somlo, M. 1976. Genetic analysis of mitochondrial biogenesis and function in Saccharomyces cerevisiae. — J. Bioenerg. 8: 93–107.

  18. Morgan, A. J., Brunner, A. and Whittaker, P. A. 1980. Protoplast fusion in a petite-negative yeast Kluyveromyces lactis. — Curr. Gen. 2: 87–93.

  19. Morgan, A. J., Hall, J. L., Brunner, A. and Whittaker, P. A. 1979. Protoplast fusion in the study of mitochondrial genetics in the petite-negative yeast, Kluyveromyces lactis. p. 93–98. In L. Ferenczy and G. L. Farkas (eds), Advances in Protoplast Research. — Pergamon Press, Oxford.

  20. Putrament, A., Baranowska, H., Ejchart, A. and Jachymczyk, W. 1977. Manganese mutagenesis in yeast VI. Mn2+ uptake, mitDNA replication and ER induction. Comparison with other divalent cations. — Mol. Gen. Genet. 151: 69–76.

  21. Sipiczki, M. and Ferenczy, L. 1977. Protoplast fusion of Schizosaccharomyces pombe auxotrophic mutants of identical mating-type. — Mol. Gen. Genet. 151: 77–81.

  22. Wickerham, L. J. 1955. New materials and procedures for genetic studies of yeasts. — Nature (London) 176: 22.

  23. Wolf, K., Del Giudice, L. and Kaudewitz, F. 1979. Promotion of uniparental inheritance of mitochondrial drug resistance by delayed division of yeast zygotes. — Mol. Gen. Genet. 176: 301–302.

  24. Yamane, T. 1973. Statistics: An Introductory Analysis, Third edition. — Harper International, London, p. 813–826.

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Allmark, B.M. Isolation of chloramphenicol-resistant mutants of Kluyveromyces lactis and characterization by mitotic segregation analysis of fused hybrids. Antonie van Leeuwenhoek 50, 167–175 (1984). https://doi.org/10.1007/BF00400178

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Keywords

  • Manganese
  • Bromide
  • Ethidium
  • Ethidium Bromide
  • Lactis