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Marine Biology

, Volume 103, Issue 4, pp 495–501 | Cite as

Effects of sublethal and lethal copper levels on hemolymph acid-base balance and ion concentrations in the shore crab Carcinus maenas kept in undiluted sea water

  • F. Boitel
  • J. -P. Truchot
Article

Abstract

Hemolymph acid-base balance and ion concentrations were measured in shore crabs, Carcinus maenas (L.), kept in fullstrength sea water (≈33‰ S) and exposed to sublethal (0.5 mg l-1) and lethal (1 and 2 mg l-1) levels of copper in water. The study was conducted throughout the year 1987 on crabs collected near Arcachon (France). Whatever the dose, waterborne copper induced metabolic acidosis without marked changes of hemolymph ion concentrations. At the sublethal copper level, the acidosis was non-lactic and partly compensated by transitory hypocapnia. Complete recovery was observed within 20 d. At intermediate and lethal copper levels, this primary acidosis was later reinforced by hypercapnia and accumulation of lactic acid, indicating that restriction of respiratory gas exchange is the probable cause of death. A steep increase in the hemolymph calcium concentration before death suggests buffering of the acidosis by calcium-carbonate stores from the exoskeleton.

Keywords

Lactic Acid Metabolic Acidosis Marked Change Complete Recovery Steep Increase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature cited

  1. Anderson, D. H., Robinson, R. J. (1946). Rapid electrometric determination of the alkalinity of seawater. Ind. Engng Chem. analyt. Edn 18: 767–769Google Scholar
  2. Astrup, P. (1956). A simple electrometric technique for the determination of carbon dioxide tension in blood and plasma, total content of carbon dioxide in plasma, and bicarbonate content in “separated” plasma at a fixed carbon dioxide tension (40 mm Hg). Scand. J. clin. Lab. Invest. 8: 33–43Google Scholar
  3. Baker, J. T. P. (1969). Histological and electron microscopical observations on copper poisoning in the winter flounder (Pseudopleuronectes americanus). J. Fish. Res. Bd Can. 26: 2785–2793Google Scholar
  4. Bjerregaard, P., Vislie, T. (1986). Effect of copper on ion-and osmoregulation in the shore crab Carcinus maenas. Mar. Biol. 91: 69–76Google Scholar
  5. Cardeilhac, P. T., Simpson, C. F., Lovelock, R. L., Yosha, S. F., Calderwood, H. W., Gudat, J. C. (1979). Failure of osmoregulation with apparent potassium intoxication in marine teleosts: a primary effect of copper. Aquaculture, Amsterdam 17: 231–239Google Scholar
  6. Culberson, C., Pytkowicz, R. M., Hawley, J. E. (1970). Sewater alkalinity determination by the pH method. J. mar. Res. 28: 15–21Google Scholar
  7. Dejours, P., Armand, J. (1980). Hemolymph acid-base balance of the crayfish Astacus leptodactylus as a function of the oxygenation and acid-base balance of the ambient water. Respir. Physiol. 41: 1–11Google Scholar
  8. Depledge, M. H. (1984). Disruption of circulatory and respiratory activity in shore crabs [Carcinus maenas (L.)] exposed to heavy metal pollution. Comp. Biochem. Physiol 78C: 445–459Google Scholar
  9. Hughes, G. M., Adeney, R. J. (1977). The effecs of zinc on the cardiac and ventilatory rhythms of rainbow trout (Salmo gairdneri, Richardson) and their responses to environmental hypoxia. Wat. Res. 11: 1069–1077Google Scholar
  10. Jensen, F. B., Weber, R. E. (1987). Internal hypoxia-hypercapnia in tench exposed to aluminium in acid water: effects on blood gas transport, acid-base status and electrolyte composition in arterial blood. J. exp. Biol. 127: 427–442Google Scholar
  11. McKim, J. M., Christensen, G. M., Hunt, E. P. (1970). Changes in the blood of brook trout (Salvelinus fontinalis) after short-term and long-term exposure to copper. J. Fish. Res. Bd Can. 27: 1883–1889Google Scholar
  12. Lauren, D. J., McDonald, D. G. (1985). Effects of copper on branchial ionoregulation in the rainbow trout, Salmo gairdneri Richardson. J. comp. Physiol. (Sect. B) 155: 635–644Google Scholar
  13. Lewis, S. D., Lewis, W. M. (1971). The effect of zinc and copper on the osmolality of blood serum of the channel catfish, Ictalurus punctatus Rafinesque, and golden shiner, Notemigonus crysoleucas Mitchill. Trans. Am. Fish. Soc. 4: 639–643Google Scholar
  14. Lorz, H. W., McPherson, B. P. (1976). Effects of copper or zinc in fresh water on the adaptation to sea water and ATPase activity, and the effects of copper on migratory disposition of coho salmon (Oncorhynchus kisutch). J. Fish. Res. Bd Can. 33: 2023–2030Google Scholar
  15. Olson, K. R., Fromm, P. O., Frantz, W. L. (1973). Ultrastructural changes of rainbow trout gills exposed to methyl mercury or mercuric chloride. Fedn Proc. Fedn Am. Socs exp. Biol. 32: p. 261Google Scholar
  16. Skidmore, J. F., Tovell, P. W. A. (1972). Toxic effects of zinc sulphate on the gills of rainbow trout. Wat. Res. 6: 217–230Google Scholar
  17. Spry, D. J., Wood, C. M. (1985). Ion flux rates, acid-base status, and blood gases in rainbow trout, Salmo gairdneri, exposed to toxic zinc in natural soft water. Can. J. Fish. aquat. Sciences 42: 1332–1341Google Scholar
  18. Stagg, R. M., Shuttleworth, T. J. (1982a). The accumulation of copper in Platichthys flesus L. and its effects on plasma electrolyte concentrations. J. Fish Biol. 20: 491–500Google Scholar
  19. Stagg, R. M., Shuttleworth, T. J. (1982b). The effects of copper on ionic regulation by the gills of the seawater-adapted flounder (Platichthys flesus L.). J. comp. Physiol. (Sect. B) 149: 83–90Google Scholar
  20. Thurberg, F. P., Dawson, M. A., Collier, R. S. (1973). Effects of copper and cadmium on osmoregulation and oxygen consumption in two species of estuarine crabs. Mar. Biol. 23: 171–175Google Scholar
  21. Truchot, J. P. (1976). Carbon dioxide combining properties of the blood of the shore crab Carcinus maenas (L.): carbon dioxide solubility coefficient and carbonic acid dissociation constants. J. exp. Biol. 64: 45–57Google Scholar
  22. Zanders, I. P. (1980). Regulation of blood ions in Carcinus maenas (L.). Comp. Biochem. Physiol. 65A: 97–108Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • F. Boitel
    • 1
  • J. -P. Truchot
    • 1
  1. 1.Laboratoire de Neurobiologie et Physiologie Comparées, CNRS UA 1126Université de Bordeaux IArcachonFrance

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