Antonie van Leeuwenhoek

, Volume 48, Issue 1, pp 25–38 | Cite as

Taxonomic studies on Filobasidiella species and their anamorphs

  • K. J. Kwon-Chung
  • J. E. Bennett
  • J. C. Rhodes


The taxonomy of Filobasidiella neoformans Kwon-Chung and F. bacillispora Kwon-Chung and their anamorphs were reinvestigated. Although the cross between the type cultures of the two species failed to produce viable basidio-spores, another pair of isolates did yield viable basidiospores. The segregation of phenotypic markers among the tetrads isolated from this interspecific cross proved that meiosis had occurred. On the basis of other previously known differences and the present genetic study, the two species are now considered to be two varieties of the species, F. neoformans. The anamorph of F. neoformans var. neoformans grew well at 37°C in vitro and produced fatal infection in mice while that of F. neoformans var. bacillispora grew poorly at 37°C and failed to produce fatal infection in mice. Cryptococcus bacillisporus Kwon-Chung et Bennett is regarded as a synonym of C. neoformans var. gattii Vanbreuseghem et Takashio.


Genetic Study Taxonomic Study Phenotypic Marker Interspecific Cross Fatal Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aulakh, H. S., Straus, S. E. and Kwon-Chung, K. J. 1981. Genetic relatedness of Filobasidiella neoformans (Cryptococcus neoformans) and Filobasidiella bacillispora (Cryptococcus bacillisporus) as determined by DNA base composition and sequence homology studies. — Int. J. Syst. Bacteriol. 31: 97–103.Google Scholar
  2. Bennett, J. E., Kwon-Chung, K. J. and Howard, D. H. 1977. Epidemiologic differences among serotypes of Cryptococcus neoformans. — Amer. J. Epidemiol. 105: 582–586.Google Scholar
  3. Bennett, J. E., Kwon-Chung, K. J. and Theodore, T. 1978. Biochemical differences between serotypes of Cryptococcus neoformans. — Sabouraudia 16: 167–174.PubMedGoogle Scholar
  4. Bhattacharjee, A. K., Kwon-Chung, K. J. and Glaudemans, C. P. J. 1978. On the structures of the capsular polysaccharide from Cryptococcus neoformans serotype C. — Immunochemistry 15: 673–679.PubMedGoogle Scholar
  5. Bhattacharjee, A. K., Kwon-Chung, K. J. and Glaudemans, C. P. J. 1979. The structure of the capsular polysaccharide from Cryptococcus neoformans serotype D. — Carbohydr. Res. 73: 183–192.CrossRefPubMedGoogle Scholar
  6. Bhattacharjee, A. K., Kwon-Chung, K. J. and Glaudemans, C. P. J. 1980. Structural studies on the major, capsular polysaccharide from Cryptococcus bacillisporus Serotype B. — Carbohydr. Res. 82: 103–111.CrossRefPubMedGoogle Scholar
  7. Bhattacharjee, A. K., Kwon-Chung, K. J. and Glaudemans, C. P. J. 1981. Capsular polysaccharides from a parent strain and from a possible, mutant strain of Cryptococcus neoformans serotype A. — Carbohydr. Res. 95: 237–248.CrossRefPubMedGoogle Scholar
  8. Cherniak, R., Reiss, e., Slodki, M. E., Plattner, R. D. and Blumer, S. O. 1980. Structure and antigenic activity of the capsular polysaccharide of Cryptococcus neoformans serotype A. —Mol. Immunol. 17: 1025–1032.CrossRefPubMedGoogle Scholar
  9. Henderson, D. K., Edwards, J. E., Jr., Dismukes, W. E. and Bennett, J. E. 1981. Meningitis produced by different serotypes of Cryptococcus neoformans. — Abstracts of the annual meeting of the American Society for Microbiology, p. 315.Google Scholar
  10. Kwon-Chung, K. J. 1975. A new genus Filobasidiella, the perfect state of Cryptococcus neoformans. — Mycologia 67: 1197–1200.PubMedGoogle Scholar
  11. Kwon-Chung, K. J. 1976. A new species of Filobasidiella, the sexual state of Cryptococcus neoformans B and C serotypes. — Mycologia 68: 942–946.Google Scholar
  12. Kwon-Chung, K. J. 1978. Heterothallism vs. self-fertile isolates of Filobasidiella neoformans (Cryptococcus neoformans). p. 204–213. — Scientific publication No. 356, Pan American Health Organization, Washington, D.C.Google Scholar
  13. Kwon-Chung, K. J. and Bennett, J. E. 1978. Distribution of α and a mating types of Cryptococcus neoformans among natural and clinical isolates. — Amer. J. Epidemiol. 108: 337–340.Google Scholar
  14. Kwon-Chung, K. J., Bennett, J. E. and Theodore, T. S. 1978. Cryptococcus bacillisporus sp. nov. Serotype B-C of Cryptococcus neoformans. — Int. J. Syst. Bacteriol. 28: 616–620.Google Scholar
  15. Merrifield, E. H. and Stephen, A. M. 1980. Structural investigations of two capsular polysaccharides from Cryptococcus neoformans. — Carbohydr. Res. 86: 69–76.CrossRefGoogle Scholar
  16. Polacheck, I. and Kwon-Chung, K. J. 1980. Creatinine metabolism in Cryptococcus neoformans and Cryptococcus bacillisporus. — J. Bacteriol. 142: 15–20.PubMedGoogle Scholar
  17. Price, C. W., Fuson, G.B. and Phaff, H. J. 1978. Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia. — Microbiol. Rev. 42: 161–193.PubMedGoogle Scholar
  18. Salkin, I. F. and Hurd, N. J. 1981. A new medium for differentiation of Cryptococcus neoformans serotype pairs. — J. Clin. Microbiol. in press.Google Scholar
  19. Schmeding, K. A., Jong, S. C. and Hugh, R. 1981. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans) — Current Microbiol. 5: 133–138.Google Scholar
  20. Vanbreuseghem, R. and Takashio, M. 1970. An atypical strain of Cryptococcus neoformans (Sanfelice) Vuillemin 1894. II. Crytococcus neoformans var. gattii var. nov. — Ann. Soc. Belg. Med. Trop. 50: 695–702.PubMedGoogle Scholar

Copyright information

© H. Veenman & Zonen B.V. 1982

Authors and Affiliations

  • K. J. Kwon-Chung
    • 1
  • J. E. Bennett
    • 1
  • J. C. Rhodes
    • 1
  1. 1.Laboratory of Clinical Investigation, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaMarylandUSA

Personalised recommendations