Antonie van Leeuwenhoek

, Volume 42, Issue 1–2, pp 145–155 | Cite as

Autolysis of high-GC isolates of Pseudomonas putrefaciens

  • R. E. Levin
  • Camilla Van Sickle


High-GC isolates of P. putrefaciens undergo extensive autolysis after growth, resulting in a marked decrease in turbidity and the release of high-molecular-weight DNA which imparts a high viscosity to culture broths. The native DNA released is resistant to attack by the exocellular DNase activity of the culture broths. Autolysis is inhibited by a pH of 6.0 and the presence of 0.001 m Mg++ or Ca++, and is enhanced by elevated pH values and temperatures. This autolytic phenomenon in broth cultures readily distinguishes high- from low-GC isolates. The latter do not exhibit autolysis.


High Viscosity Marked Decrease Culture Broth DNase Activity Autolytic Phenomenon 
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  1. Arvidson, S., Holme, T. and Wadström, T. 1970. Formation of bacteriolytic enzymes in batch and continuous culture of Staphylococcus aureus. — J. Bacteriol. 104: 227–233.Google Scholar
  2. Chen, T. C. and Levin, R. E. 1974. Taxonomic significance of phenethyl alcohol production by Achromobacter isolates from fishery sources. — Appl. Microbiol. 28: 681–687.Google Scholar
  3. Hebeler, B. H. and Young, F. E. 1975. Autolysis of Neisseria gonorrhoeae. — J. Bacteriol. 122: 385–392.Google Scholar
  4. Levin, R. E. 1972. Correlation of DNA base composition and metabolism of Pseudomonas putrefaciens isolates from food, human clinical specimens, and other sources. — Antonie van Leeuwenhoek 38: 121–127.Google Scholar
  5. Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. — J. Mol. Biol. 3: 208–218.Google Scholar
  6. Mohan, R. R., Kronish, D. P., Pianotti, R. S., Epstein, R. L. and Schwartz, B. S. 1965. Autolytic mechanism for spheroplast formation in Bacillus cereus and Escherichia coli. —J. Bacteriol. 90: 1355–1364.Google Scholar
  7. Murakawa, T. 1973. Slime production by Pseudomonas aeruginosa. III. Purification of slime and its physicochemical properties. — Japan J. Microbiol. 17: 273–281.Google Scholar
  8. Sadovski, A. Y. and Levin, R. E. 1969. Extracellular nuclease activity of fish spoilage bacteria, fish pathogens, and related species. — Appl. Microbiol. 17: 787–789.Google Scholar
  9. Shockman, G. D. 1965. Symposium on the fine structure and replication of bacteria and their parts. IV. Unbalanced cell-wall synthesis: autolysis and cell-wall thickening. — Bacteriol. Rev. 29: 345–358.Google Scholar
  10. Young, F. E. 1966. Autolytic enzyme associated with cell walls of Bacillus subtilis. — J. Biol. Chem. 241: 3462–3467.Google Scholar

Copyright information

© H. Veenman en Zonen 1976

Authors and Affiliations

  • R. E. Levin
    • 1
  • Camilla Van Sickle
    • 1
  1. 1.Department of Food Science and NutritionUniversity of MassachusettsAmherstU.S.A.

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