Autolysis of high-GC isolates of Pseudomonas putrefaciens
High-GC isolates of P. putrefaciens undergo extensive autolysis after growth, resulting in a marked decrease in turbidity and the release of high-molecular-weight DNA which imparts a high viscosity to culture broths. The native DNA released is resistant to attack by the exocellular DNase activity of the culture broths. Autolysis is inhibited by a pH of 6.0 and the presence of 0.001 m Mg++ or Ca++, and is enhanced by elevated pH values and temperatures. This autolytic phenomenon in broth cultures readily distinguishes high- from low-GC isolates. The latter do not exhibit autolysis.
KeywordsHigh Viscosity Marked Decrease Culture Broth DNase Activity Autolytic Phenomenon
Unable to display preview. Download preview PDF.
- Arvidson, S., Holme, T. and Wadström, T. 1970. Formation of bacteriolytic enzymes in batch and continuous culture of Staphylococcus aureus. — J. Bacteriol. 104: 227–233.Google Scholar
- Chen, T. C. and Levin, R. E. 1974. Taxonomic significance of phenethyl alcohol production by Achromobacter isolates from fishery sources. — Appl. Microbiol. 28: 681–687.Google Scholar
- Hebeler, B. H. and Young, F. E. 1975. Autolysis of Neisseria gonorrhoeae. — J. Bacteriol. 122: 385–392.Google Scholar
- Levin, R. E. 1972. Correlation of DNA base composition and metabolism of Pseudomonas putrefaciens isolates from food, human clinical specimens, and other sources. — Antonie van Leeuwenhoek 38: 121–127.Google Scholar
- Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. — J. Mol. Biol. 3: 208–218.Google Scholar
- Mohan, R. R., Kronish, D. P., Pianotti, R. S., Epstein, R. L. and Schwartz, B. S. 1965. Autolytic mechanism for spheroplast formation in Bacillus cereus and Escherichia coli. —J. Bacteriol. 90: 1355–1364.Google Scholar
- Murakawa, T. 1973. Slime production by Pseudomonas aeruginosa. III. Purification of slime and its physicochemical properties. — Japan J. Microbiol. 17: 273–281.Google Scholar
- Sadovski, A. Y. and Levin, R. E. 1969. Extracellular nuclease activity of fish spoilage bacteria, fish pathogens, and related species. — Appl. Microbiol. 17: 787–789.Google Scholar
- Shockman, G. D. 1965. Symposium on the fine structure and replication of bacteria and their parts. IV. Unbalanced cell-wall synthesis: autolysis and cell-wall thickening. — Bacteriol. Rev. 29: 345–358.Google Scholar
- Young, F. E. 1966. Autolytic enzyme associated with cell walls of Bacillus subtilis. — J. Biol. Chem. 241: 3462–3467.Google Scholar