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Interactions of species in an Anabaena flos-aquae association from the Plußsee (East-Holstein, Federal Republic of Germany)

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Summary

Fluorescent probes have been applied to study by epifluorescence microscopy interactions between species in an Anabaena flos-aquae association, derived from a freshwater community. Glycoconjugates (macromolecules containing carbohydrate residues) have been shown to play a vital role in recognition processes and in formation of attachment sites between cells. By use of a group of proteins (lectins) with specific affinities to certain sugar residues we were able to clucidate specific patterns expressed at cellular surfaces, thereby indicating the physiological state of a cell. Low molecular weight fluorochromes, like the fluorescent brightener calcofluor white have been shown to be extremely useful tools for visualizing structural entities (and species) which otherwise escape notice by microscopy. We have demonstrated that the decline of the bloom of Anabaena flos-aquae was caused or at least accelerated by action of a fungus (chytrid). We also proved that the shell (lorica) of a choanoflagellate most likely is built from chitin.

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References

  1. Al-Maadhidi J, Henrikson E (1980) Effects of fungi Trichoderma harzianum and Aspergillus flavus on the nitrogen fixation and growth of the alga Anabaena variabilis. Oikos 35:115–119

  2. Callow JA (1977) Recognition, resistance and the role of plant lectins in host-parasite interactions. Adv Bot Res 4:1–49

  3. Canter HM (1972) A guide to the fungi occuring on planktonic bluegreen algae. In: Desikachary TV (ed) Taxonomy and Biology of Bluegreen algae. University of Madras Press Madras, pp 145–159

  4. Canter HM (1979) Fungal and protozoan parasites and their importance in the ecology of the phytoplankton. FBA Ann Rep 47:43–50

  5. Carmichael WW, Gorham PP (1977) Factors influencing the toxicity and animal susceptibility of Anabaena flos-aquae (cyanophyta) blooms. J Phycol 13:97–101

  6. Dann O, Bergen G, Demant E, Volz G (1971) Trypanocide diamidine des 2-Phenyl-benzofurans, 2-Phenyl-indens und 2-Phenylindols. Ann Chem 749:68–89

  7. Doflein F (1916) Lehrbuch der Protozoenkunde, 4. Aufl. Gustav Fischer Verlag, Jena

  8. Fogg GE (1952) The production of extracellular nitrogeneous substances by a bluegreen alga. Proc R Soc London Ser B, 139, 372–392

  9. Gorham PR (1964) Toxic algae. In: Jackson DF (ed) Algae and Man. Plenum Press, New York, pp. 307–336

  10. Hellebust JA (1974) Extracellular products. In: Stewart WDP (ed) Algal Physiology and Biochemistry. Blackwell Scientific Publ, Oxford, pp 838–863

  11. Herbst V, Overbeck J (1978) Metabolic coupling between the alga Oscillatoria redekei and accompanying bacteria. Naturwissenschaften 65:598

  12. Hickel B (1975) Changes in phytoplankton species composition since 1894 in two lakes of East-Holstein, Germany. Verh Internat Verein Limnol 19:1229–1240

  13. Lange W (1967) Effect of carbohydrates on the symbiotic growth of planktonic blue-green algae with bacteria. Nature 215:1277–1278

  14. Lin MS, Alfi OS, Donnell GN (1976) Differential fluorescence of sister chromatids with 4′,6-diamidino-2-phenylindole. Canad J Genet Cytol 18:545–547

  15. Mirelman D, Galun E, Sharon N, Lotan R (1975) Inhibition of fungal growth by wheat germ agglutinin. Nature 256:414–416

  16. Müller U, v. Sengbusch P (1983) Visualization of aquatic fungi (Chytridiales) parasitizing on algae by means of induced fluorescence. Arch Hydrobiol (in press)

  17. Nalewajko C, Dunstall TG, Shear H (1976) Kinetics of extracellular release in axenic algae and in mixed algal-bacterial cultures: significance in estimation of total (gross) phytoplankton excretion rates. J Phycol 12:1–5

  18. Ohle W (1962) Der Sauerstoffhaushalt der Seen als Grundlage einer allgemeinen Stoffwechseldynamik der Gewässer. Kiel Meeresforsch 18:107–120

  19. Overbeck J (1968) Prinzipielles zum Vorkommen der Bakterien im See. Mitt Internat Verein Limnol 14:134–144

  20. Pearl HW (1976) Specific associations of the bluegreen algae Anabaena and Aphanizomenon with bacteria in freshwater blooms. J Phycol 12:431–435

  21. Pearl HW (1982) Interactions with bacteria. In: Carn NG, Whitton BA (eds) The Biology of Cyanobacteria. Blackwell Scientific Publ, Oxford, pp 441–461

  22. Pearl HW, Kellar PE (1978) Significance of bacterial-Anabaena (Cyanophyceae) associations with respect to N2 fixation in freshwater. J Phycol 14:254–260

  23. Pistole TG (1981) Interaction of bacteria and fungi with lectins and lectin-like substances. Ann Rev Microbiol 35:85–112

  24. Porter KG, Feig YS (1980) The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25:943–948

  25. Redhead K, Wright SJL (1980) Lysis of the cyanobacterium Anabaena flos-aquae by antibiotic producing fungi. J Gen Microbiol 119:95–101

  26. Rohringer R, Kim WK, Samborski DJ, Howes NK (1972) Calcofluor: an optical brightener for fluorescence microscopy of fungal plant parasites in leaves. Phytopathology 67:808–810

  27. Sengbusch P von, Hechler J, Müller U (1983) Molecular architecture of fungal cell walls: An approach by use of fluorescent markers. Eur J Cell Biol (in press)

  28. Sengbusch P von, Mix M, Wachholz I, Manshard E (1982) FITC-labeled lectins and calcofluor white ST as probes for the investigation of the molecular architecture of cell surfaces. Studies on Conjugatophycean species. Protoplasma 111:38–52

  29. Sengbusch P von, Müller U (1983) Distribution of glycoconjugates at algal cell surfaces as monitored by FITC-conjugated lectins. Protoplasma 114:103–113

  30. Sparrow FK (1960) Aquatic Phycomycetes. The University of Michigan Press, Ann Arbor

  31. Stein F von (1878) Der Organismus der Infusorientiere III, 1. Hälfte, Engelhard Verlag, Leipzig, pp 1–154

  32. Stewart WDP, Daft MJ (1977) Microbial pathogens of Cyanophycean blooms. Adv Aquatic Microbiol 1:177–218

  33. Tsao PH (1970) Applications of the vital fluorescent labelling technique with brighteners to studies of the saprophytic behaviour of Phytophthora in soil. Soil Biol Biochem 2:247–256

  34. Utermöhl H (1925) Limnologische Planktonstudien. Die Besiedlung ostholsteinischer Seen mit Schwebpflanzen. Arch Hydrobiol Suppl 5:1–527

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Correspondence to Peter v. Sengbusch.

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Studies by use of fluorescent Markers

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Müller, U., Sengbusch, P.v. Interactions of species in an Anabaena flos-aquae association from the Plußsee (East-Holstein, Federal Republic of Germany). Oecologia 58, 215–219 (1983). https://doi.org/10.1007/BF00399219

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Keywords

  • Sugar
  • Macromolecule
  • Chitin
  • Fluorescent Probe
  • Specific Pattern