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Effect of magnesium and ATP on ATPase of sugarcane vacuoles

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Abstract

Kinetic analysis of the Mg2+-dependence of tonoplast ATPase from suspension-cultured cells of sugarcane showed that the enzyme activity increased with increasing magnesium concentrations till 1–3 mM and then decreased consideably for higher concentrations. This kinetic could be explained by the assumption that MgATP2- is the substrate of ATPase: MgATP2- concentration increases with increasing concentration of magnesium till, at high concentrations of magnesium, Mg2ATP is formed. No evidence for a direct role of Mg2+ as activator or inhibitor was found. These data corroborate previous findings that MgATP2- is the sole substrate of the vacuolar ATPase of sugarcane (Thom and Komor 1984). High concentrations of ATP seemed to inhibit the ATPase. This result, however, could be traced back to interference of ATP with the Fiske-Subbarow method of phosphate determination. After adjustment of the test conditions, inhibition by ATP was no longer found. Reported data for ATPases of other plant materials, showing inhibition of enzyme activity with high magnesium or ATP concentrations, might be explicable in a similar way.

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Abbreviations

Mes:

2-(N-morpholino)ethane+Sulfonic acid

References

  1. Admon, A., Jacoby, B., Goldschmidt, E.E. (1981) Some characteristics of the Mg-ATPase of isolated red beet vacuoles. Plant Sci. Lett. 22, 89–96

  2. Balke, N.E., Hodges, T.K. (1975) Plasma membrane adenosine triphosphatase of oat roots. Activation and inhibition by Mg2+ and ATP. Plant Physiol. 55, 83–86

  3. Beffagna, N., Marré, E., Cocucci, S.M. (1979) Cation-activated ATPase activity of plasmalemma-enriched membrane preparations from maize coleoptiles. Planta 146, 387–391

  4. Bowman, E.J., Bowman, B.J. (1982) Identification and properties of an ATPase in vacuolar membranes of Neurospora crassa. J. Bacteriol. 151, 1326–1337

  5. Bowman, B.J., Slayman, C.W. (1977) Characterization of plasma membrane adenosine triphosphatase of Neurospora crussa. J. Biol. Chem. 252, 3357–3363

  6. Briskin, D.P., Poole, R.J. (1983) Role of magnesium in the plasma membrane ATPase of red beet. Plant Physiol. 71, 969–971

  7. D'Auzac, J. (1977) ATPase membranaire de vacuoles lysosomales: les lutoides du latex d'Hevea brasiliensis. Phytochemistry 16, 1881–1885

  8. Dufour, J., Goffeau, A. (1980) Molecular and kinetic properties of the purified plasma membrane ATPase of the yeast Schizosaccharomyces pombe Eur. J. Biochem. 105, 145–154

  9. Fiske, C.H., Subbarow, Y. (1925) The colorimetric determination of phosphorus. J. Biol. Chem. 66, 375–400

  10. Goffeau, A., Slayman, C.W. (1981) The proton-translocating ATPase of the fungal plasma membrane. Biochim. Biophys. Acta 639, 197–223

  11. Heinonen, J.K., Lahti, R.J. (1981) A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Anat. Biochem. 113, 313–317

  12. Kakinuma, Y., Oshumi, Y., Anraku, Y. (1981) Properties of H+-translocating adenosine triphosphatase in vacuolar membranes of Saccharomyces cerevisiae. J. Biol. Chem. 256, 10859–10863

  13. Kasamo, K. (1979) Characterization of membrane-bound Mg++-activated ATPase isolated from the lower epidermis of tobacco leaves. Plant Cell Physiol. 20, 281–292

  14. Leigh, R.A., Walker, R.A. (1980) A method for preventing sorbitol interference with the determination of inorganic phosphate. Anal. Biochem. 106, 279–284

  15. Lin W., Wagner, G.J., Siegelman, H.W., Hind, G. (1977) Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue. Biochim. Biophys. Acta 465, 110–117

  16. Lindberg, O., Ernster, L. (1956) Determination of organic phosphorus compounds by phosphate analysis. Methods Biochem. Anal. 3, 1–24

  17. McMurchie, E.J., Pomeroy, K. (1981) Isolation and properties of ion stimulated ATPase activity associated with cauliflower plasma membranes. Plant Physiol. 68, 626–630

  18. Nickell, L.G., Maretzki, A. (1969) Growth of suspension cultures of sugarcane cells in chemically defined media. Physiol. Plant. 22, 117–125

  19. Perlin, D.S., Spanswick, R.M. (1981) Characterization of ATPase activity associated with corn leaf plasma membranes. Plant Physiol. 68, 521–526

  20. Rungie, J.M., Wiskich, J.T. (1973) Salt-stimulated adenosine triphosphatase from smooth microsomes of turnip. Plant Physiol. 51, 1064–1068

  21. Storer, A.C., Cornish-Bowden, A. (1976) Concentration of MgATP2- and other ions in solution. Calculation of the true concentration of species present in mixtures of associating ions. Biochem. J. 159, 1–5

  22. Sze, H. (1982) Characterization of nigericin-stimulated ATPase from sealed microsomal vesicles of tobacco callus. Plant Physiol. 70, 498–505

  23. Thom, M., Komor, E. (1984) Role of the ATPase of sugarcane vacuoles in energization of the tonoplast. Eur. J. Biochem. 138, 93–99

  24. Thom, M., Maretzki, A., Komor, E. (1982) Vacuoles from sugarcane suspension cultures. I. Isolation and partial characterization. Plant Physiol. 69, 1315–1319

  25. Travis, R.L., Booz, M. (1979) Partial characterization of a potassium-stimulated adenosine triphosphatase from the plasma membrane of meristematic and mature soybean root tissue. Plant Physiol. 63, 573–577

  26. Wagner, S.J. (1981) Enzymic and protein character of tonoplast from Hippeastrum vacuoles. Plant Physiol. 68, 499–503

  27. Walker, R.A., Leigh, R.A. (1981) Characterization of a saltstimulated ATPase activity associated with vacuoles isolated from storage root of red beet (Beta vulgaris L.). Planta 153, 140–149

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Correspondence to Ewald Komor.

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Thom, M., Komor, E. Effect of magnesium and ATP on ATPase of sugarcane vacuoles. Planta 161, 361–365 (1984). https://doi.org/10.1007/BF00398727

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Key words

  • ATPase
  • ATP ion
  • Magnesium (ATPase activity)
  • Saccharum (vacuole)