Journal of Biomolecular NMR

, Volume 4, Issue 6, pp 787–797 | Cite as

Resonance assignment of methionine methyl groups and χ3angular information from long-range proton—carbon and carbon—carbon J correlation in a calmodulin—peptide complex

  • Ad Bax
  • Frank Delaglio
  • Stephan Grzesiek
  • Geerten W. Vuister
Research Paper


Several simple 3D experiments are used to provide J correlations between methionine Cε methyl carbons and either the CγH2 protons or Cβ and Cγ. The intensity of the J correlations provides information on the size of the three-bond J couplings and thereby on the χ3 torsion angle. In addition, a simple 3D version of the HMBC experiment provides a sensitive link between the CεH3 methyl protons and Cγ. The methods are demonstrated for a 20 kDa complex between calmodulin and a 26-residue peptide fragment of skeletal muscle myosin light chain kinase.


Calmodulin Carbon-carbon J coupling Carbon-proton J coupling HMBC Methionine Protein NMR 3D NMR Isotope labeling 


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Copyright information

© ESCOM Science Publishers B.V 1994

Authors and Affiliations

  • Ad Bax
    • 1
  • Frank Delaglio
    • 1
  • Stephan Grzesiek
    • 1
  • Geerten W. Vuister
    • 1
  1. 1.Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaU.S.A.

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