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Journal of Comparative Physiology B

, Volume 162, Issue 2, pp 148–152 | Cite as

Identification of the yolk receptor protein in oocytes of Nereis virens (Annelida, Polychaeta) and comparison with the locust vitellogenin receptor

  • J. Hafer
  • A. Fischer
  • H. J. Ferenz
Article

Summary

In oviparous animals large amounts of yolk proteins of extraovarian origin are accumulated by developing oocytes during vitellogenesis. The yolk protein precursors, the vitellogenins (VTG), are transported into the oocytes by receptor-mediated endocytosis. In oocytes of the polychaetous annelid, Nereis virens, the receptor protein for VTG was visualized by ligand blotting studies as a protein with an apparent molecular mass of 190 kDa under non-reducing conditions. Anti-Locusta VTG receptor antibodies recognize the Nereis VTG receptor protein. The Nereis VTG receptor protein binds Locusta and Schistocerca VTG; the VTG receptor proteins of both locust species bind the Nereis vitellin. These results indicate the conservation of structural elements important for internalization of VTG.

Key words

Vitellogenesis Receptor-mediated endocytosis Vitellogenin receptor protein Nereis Locusta Schistocerca 

Abbreviations

CHAPS

3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane-sulphonic acid

HBS

HEPES-buffered saline

PAP

peroxidase-anti-peroxidase

SDS-PAGE

sodium dodecylsulphate polyacrylamide gel electrophoresis

TRIS, TBS

TRIS-buffered saline

VT

vitellin

VTG

vitellogenin

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • J. Hafer
    • 1
  • A. Fischer
    • 2
  • H. J. Ferenz
    • 1
  1. 1.Insect Physiology GroupUniversity of OldenburgOldenburgGermany
  2. 2.Institute of ZoologyUniversity of MainzMainzGermany

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