Aqueous extract of betel-nut of North-East India induces DNA-strand breaks and enhances rate of cell proliferation in vitro
- 39 Downloads
An aqueous extract of betel-nut has been found to be able to induce strand breaks in DNA of mouse kidney cells in vitro. It has been also found to be able to enhance the rate of cell division at a dose of 100 μg/ml while a higher dose of 250 μg/ml was extremely toxic to the cells. Compared with arecoline (10 μg/ml), the aqueous extract of betel-nut seems to be a more potent carcinogen to mouse kidney cells in vitro.
Key wordsBetel-nut DNA-strand breaks Carcinogenesis In vitro
Unable to display preview. Download preview PDF.
- Burton K (1968) Determination of DNA concentration with diphenylamine. Methods Enzymol 12 B:163–166Google Scholar
- IARC (1985) International Agency for Research on Cancer, Lyons. Tobacco habits other than smoking: betel-quid and areca-nut chewing and some related nitrosamines (IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans, vol 37)Google Scholar
- Phillips HJ (1973) Dye exclusion tests for cell viability. In: Kruse PF Jr, Patterson MK (eds) Tissue culture methods and applications. Academic Press, New York p 406Google Scholar
- Rydberg B (1975) The rate of strand separation in alkali of DNA of irradiated mammalian cells. Radiat Res 66:274–287Google Scholar
- Sharp TM (1948) Alkaloids of Areca or betel nut. In: Mitchell CA (ed) Allen's commercial organic analysis, 5th edn, vol 7. Churchill, London, pp 52–57Google Scholar
- Srivastava PN, Sharan RN, Pozzi L (1982) Effect of tritiated water ingestion on mice: III. Damage at cellular vis-à-vis subcellular level monitored upt four generations. In: Reynand, C (ed) Nuclear medicine and biology Adv, vol 4, Pergamon Press, Oxford, pp 2989–2992Google Scholar
- Stich HF, Bohm B, Chatterji K, Sailo J (1983) The role of saliveborne mutagens and carcinogens in the etiology of oral and oesophageal carcinomas of betel nut and tobacco chewers. In: Stich HF (ed) Carcinogens and mutagens in the environment, vol 3, Naturally occurring compounds. CRC Press, Boca Raton, FJGoogle Scholar