, Volume 101, Issue 4, pp 296–316 | Cite as

Stomatal movement in Zea mays: Shuttle of potassium and chloride between guard cells and subsidiary cells

  • Klaus Raschke
  • Margaret Pierce Fellows


When stomates of Zea mays open K and Cl migrate from the subsidiary cells into the guard cells; when the stomates close both elements return to the subsidiary cells. Subsidiary cells function as reservoirs for K and Cl. Import of K and Cl into the guard cells and loss of both elements from the guard cells become observable 1 or 2 min after light is turned on or off, both when histochemical methods and the electron-probe microanalyzer are used for detection. Each stomatal complex of maize contains on the average 10±3×10-13 gram equivalents (eq) of K and 4±1×10-13 eq of Cl. Guard cells accumulate K in the light and CO2-free air at an average rate of 10×10-15 eq K per minute, and Cl at approximately half that rate.


Chloride Potassium Maize Average Rate Guard Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Epstein, E., Rains, D. W., Elzam, O. E.: Resolution of dual mechanisms of potassium absorption by barley roots. Proc. nat. Acad. Sci. (Wash.) 49, 648–692 (1963).Google Scholar
  2. Fischer, R. A., Hsiao, T. C.: Stomatal opening in isolated epidermal strips of Vicia faba. II. Responses to KCl concentration and the role of potassium absorption. Plant Physiol. 43, 1953–1958 (1968).Google Scholar
  3. Fujino, M.: Stomatal movement and active migration of potassium. (In Japanese.) Kagaku 29, 660–661 (1959).Google Scholar
  4. —: Role of adenosinetriphosphate and adenosinetriphosphatase in stomatal movement. Sci. Bull. Fac. Educ. Nagasaki Univ. 18, 1–47 (1967).Google Scholar
  5. Humble, G. D., Hsiao, T. C.: Specific requirement of potassium for light-activated opening of stomata in epidermal strips. Plant Physiol. 44, 230–234 (1969).Google Scholar
  6. — Raschke, K.: Stomatal opening quantitatively related to potassium transport: Evidence from electron probe analysis. Plant Physiol. 48, 447–453 (1971).Google Scholar
  7. Imamura, S.: Untersuchungen über den Mechanismus der Turgorschwankung der Spaltöffnungsschließzellen. Jap. J. Botany 12, 251–346 (1943).Google Scholar
  8. Kauss, H.: Osmoregulation mit α-Galaktosylglyceriden bei Ochromonas und Rotalgen. Ber. dtsch. bot. Ges. 82, 115–125 (1969).Google Scholar
  9. Macallum, A. B.: On the distribution of potassium in animal and vegetable cells. J. Physiol. (Lond.) 32, 95–128 (1905).Google Scholar
  10. MacRobbie, E. A. C.: The active transport of ions in plant cells. Quart. Rev. Biophys. 3, 251–294 (1970).Google Scholar
  11. Maercker, U.: Zur Kenntnis der Transpiration der Schließzellen. Protoplasma 60, 61–78 (1965).Google Scholar
  12. Metzner, H.: Die Reduktion wässriger Silbernitratlösungen durch Chloroplasten und andere Zellbestandteile. Protoplasma 41, 129–167 (1952).Google Scholar
  13. Pallaghy, C. K.: Stomatal movement and potassium transport in epidermal strips of Zea mays: The effect of CO2. Planta (Berl.) 101, 287–295 (1971).Google Scholar
  14. Raschke, K.: Eignung und Konstruktion registrierender Porometer für das Studium der Schließzellenphysiologie. Planta (Berl.) 67, 225–241 (1965).Google Scholar
  15. Sawhney, B. L., Zelitch, I.: Direct determination of potassium ion accumulation in guard cells in relation to stomatal opening in light. Plant Physiol. 44, 1350–1354 (1969).Google Scholar
  16. Stålfelt, M. G.: Endogenous diurnal rhythm in the osmotic surplus of the guard cells. Physiol. Plant. 20, 57–64 (1967).Google Scholar
  17. Yamashita, T.: Influences of potassium supply upon various properties and movement of the guard cell. Sieboldia 1, 51–70 (1952).Google Scholar
  18. Ziegler, H., Lüttge, U.: Die Salzdrüsen von Limonium vulgare. II. Die Lokalisierung des Chlorids. Planta (Berl.) 74, 1–17 (1967).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Klaus Raschke
    • 1
  • Margaret Pierce Fellows
    • 1
  1. 1.MSU/AEC Plant Research LaboratoryMichigan State UniversityEast Lansing

Personalised recommendations