Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The mechanism of crustacean salinity tolerance: Cell volume regulation by K+ and glycine effluxes

  • 159 Accesses

  • 28 Citations

Abstract

The regulation of muscle fiber K+ and free amino acid (FAA) concentrations during hypoosmotic stress was investigated in the moderately euryhaline crab Cancer irroratus. After 6 h of exposure to 60% ASW, muscle fiber K+ concentration declined from 185 mM to 140 mM. Following this, the blood glycine levels began to increase, indicating an FAA efflux from the cells. These data indicate that both muscle fiber K+ and FAA contribute to cell volume regulation in C. irroratus. The early release of K+ limits the initial rate of cell hydration. The subsequent efflux of glycine accounts for the volume regulation response of the muscle fibers. The cell volume regulatory system of C. irroratus is a coordinated use of both inorganic ions and FAA.

This is a preview of subscription content, log in to check access.

Literature cited

  1. Costa, C. J. and S. K. Pierce: Effect of divalent cations and metabolic inhibitors on Glycera red coelomocyte volume regulation and solute balance during hypoosmotic stress. J. Comp. Physiol. 151, 133–144 (1982)

  2. Dall, W.: The role of ninhydrin-positive substances in osmo-regulation in the western rock lobster, Panulirus longipes. J. exp. mar. Biol. Ecol. 19, 43–58 (1975)

  3. Freel, R. W.: Patterns of water and solute regulation in the muscle fibers of osmoconforming marine decapod crustaceans. J. exp. Biol. 72, 107–126 (1978)

  4. Freel, R. W., S. G. Medler and M. E. Clark: Solute adjustments in the coelomic fluid and muscle of a euryhaline polychaete, Neanthes succinea, adapted to various salinities. Biol. Bull. mar. biol. Lab., Woods Hole 144, 289–303 (1973)

  5. Gerard, J. F. and R. Gilles: The free amino-acid pool in Callinectes sapidus (Rathbun) tissues and its role in the osmotic intracellular regulation. J. exp. mar. Biol. Ecol. 10, 125–136 (1972)

  6. Gilles, R.: Intracellular free amino acids and cell volume regulation during osmotic stresses. In: Osmotic and volume regulation, pp 470–494. Ed. by C. B. Jørgensen and E. Skadhauge. A. Benzon. Symposium XI. Copenhagen: Munksgaard 1978

  7. Hoffman, E. K. and K. B. Hendil: The role of amino acids and taurine in isosmotic intracellular regulation in Ehrlich ascites mouse tumor cells. J. comp. Physiol. 108, 279–286 (1976)

  8. Kevers, C., A. Pequeux and R. Gilles: Effects of an hyposmotic shock on Na+, K+, and Cl- levels in isolated axons of Carcinus maenas. J. comp. Physiol. 129, 365–371 (1979)

  9. Lange, R.: Some recent work on osmotic, ionic, and volume regulation in marine animals. Oceanog mar. Biol. annu. Rev. 10, 97–136 (1972)

  10. Little, J. R.: Determination of water and electrolytes in tissue slices. Anal. Biochem. 7, 87–95 (1964)

  11. MacKnight, A. D. C. and A. Leaf: Regulation of cellular volume. Physiol. Rev. 57, 510–573 (1977)

  12. McDonald, D. G., B. R. McMahon and C. M. Wood: An analysis of acid-base disturbances in the hemolymph following strenuous activity in the dungeness crab, Cancer magister. J. exp. Biol. 79, 47–58 (1979)

  13. Pierce, S. K.: Invertebrate cell volume control mechanisms: a coordinated use of intracellular amino acids and inorganic ions as osmotic solute. Biol. Bull. mar. biol. Lab., Woods Hole 163, 405–419 (1982)

  14. Pierce, S. K. and L. M. Amende: Control mechanisms of amino acid-mediated cell volume regulation in salinity-stressed molluscs. J. exp. Zool. 215, 247–257 (1981)

  15. Pierce, S. K. and M. J. Greenberg: The nature of cellular volume regulation in marine bivalves. J. exp. Biol. 57, 681–692 (1972)

  16. Schmidt-Nielsen, B., J. L. Renfro and D. Benos: Estimation of extracellular space and intracellular ion concentrations in osmoconformers, hypo and hyper-osmoregulators. Mt. Desert Island biol. Lab. Bull. 12, 99–104 (1972)

  17. Thurston, J. H., R. E. Hauhart and J. A. Dirgo: Taurine: A role in osmotic regulation of mammalian brain and possible clinical significance. Life Sci. 26, 1561–1568 (1980)

  18. Thurston, J. H., R. E. Hauhart and E. F. Naccarato: Taurine: possible role in osmotic regulation of mammalian heart. Science, N.Y. 214, 1373–1374 (1981)

  19. Vincent-Marique, C. and R. Gilles: Changes in the amino-acid concentration in blood and muscle of Eriocheir sinensis during hypoosmotic stress. Life Sci. 9, 509–512 (1970)

  20. Warren, M. K. and S. K. Pierce: Two cell volume regulatory systems in the Limulus myocardium: an interaction of ions and quaternary ammonium compounds. Biol. Bull. mar. biol. Lab., Woods Hole 163, 504–516 (1982)

Download references

Author information

Additional information

Communicated by S. K. Pierce, College Park

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Moran, W.M., Pierce, S.K. The mechanism of crustacean salinity tolerance: Cell volume regulation by K+ and glycine effluxes. Mar. Biol. 81, 41–46 (1984). https://doi.org/10.1007/BF00397623

Download citation

Keywords

  • Muscle Fiber
  • Glycine
  • Cell Volume
  • Initial Rate
  • Regulatory System