Planta

, Volume 159, Issue 3, pp 216–221 | Cite as

Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay

  • D. Ernst
  • W. Schäfer
  • D. Oesterhelt
Article
Received:
Accepted:

Abstract

Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.

Key words

Cell culture (cytokinin content) Cytokinin isolation and quantitation Pimpinella 

Abbreviations

i6Ado

isopentenyladenosine

FW

fresh weight

GC-MS

gas chromatography-mass spectrometry

RIA

radioimmunoassay

SIM

single-ion monitoring

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • D. Ernst
    • 1
  • W. Schäfer
    • 1
  • D. Oesterhelt
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsriedFederal Republic of Germany

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