, Volume 162, Issue 1, pp 62–67 | Cite as

Identification of calmodulin in the green alga Mougeotia and its possible function in chloroplast reorientational movement

  • Gottfried Wagner
  • Petra Valentin
  • Peter Dieter
  • Dieter Marmé


A soluble protein was isolated from Mougeotia by chloropromazine-sepharose 4 B affinity chromatography. The protein matches the properties of calmodulin in terms of heat stability, Ca2+-dependent electrophoretic mobility in sodium-dodecyl-sulfate polyacrylamide gels, and its ability to activate cyclic nucleotide phosphodiesterase in a Ca2+-dependent manner. Phytochrome-mediated chloroplast reorientational movement in Mougeotia was inhibited by the calmodulin antagonist trifluoperazine, a hydrophobic compound, or N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a hydrophilic compound; 50% inhibition (IC50) of chloroplast movement is caused by 20–50 μmol l-1 trifluoperazine or 100 μmol l-1 W-7. The Ca2+-calmodulin may act as an intermediate in the chloroplast reorientational response in Mougeotia governed by phytochrome.

Key words

Calcium (chloroplast movement) Calmodulin (chloroplast movement) Chloroplast movement (MougeotiaMougeotia Phytochrome 



ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid


sodium dodecyl sulfate


N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Gottfried Wagner
    • 1
  • Petra Valentin
    • 1
  • Peter Dieter
    • 2
  • Dieter Marmé
    • 2
  1. 1.Botanisches Institut I der Justus-Liebig-UniversitätGiessenGermany
  2. 2.Institut für Biologie III der UniversitätFreiburg i. Br.Germany

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