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Lysis of bacterioids in the vicinity of the host cell nucleus in an ineffective (fix-) root nodule of soybean (Glycine max)

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Abstract

In nodules of Glycine max cv. Mandarin infected with a nod +fix- mutant of Rhizobium japonicum (RH 31-Marburg), lysis of bacteroids was observed 20 d after infection, but occurred in the region around the host cell nucleus, where lytic compartments were formed. Bacteroids, and peribacteroid membranes in other parts of the host cell remained stable until senescence (40d after infection). With two other nod+ fix- mutants of R. japonicum either stable bacteroids and peribacteroid membranes were observed throughout the cell (strain 61-A-165) or a rapid degeneration of bacteroids without an apparent lysis (strain USDA 24) occurred. The size distribution of RH 31-Marburg-infected nodules exhibited only two maxima compared with four in wild-type nodules and nodule leghaemoglobin content was found to be reduced to about one half that of the wild type. The RH 31-Marburg-nodule type is discussed in relation to the stability of the bacteroids and the peribacteroid membrane system in soybean.

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Werner, D., Mörschel, E., Kort, R. et al. Lysis of bacterioids in the vicinity of the host cell nucleus in an ineffective (fix-) root nodule of soybean (Glycine max). Planta 162, 8–16 (1984). https://doi.org/10.1007/BF00397414

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Key words

  • Glycine (root nodules)
  • Lytic compartment
  • Peribacteroid membrane
  • Rhizobium (symbiosis)
  • Root nodule (symbiosis)
  • Symbiosis (root nodules)