Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Determination of the levels of abscisic acid-glucose ester in plants


Abscisic acid-β-D-glucopyranosyl ester (ABAGE) has been measured in several plant species using 2H-labelled ABAGE as an internal standard. The effects of water stress on the levels of ABAGE and its possible physiological role are discussed. Some chemical properties of ABAGE are described.

This is a preview of subscription content, log in to check access.



abscisic acid


abscisic acid-β-D-glucopyranosyl ester


high-performance liquid chromatography


multiple-ion monitoring


  1. Dewdney, S.J., McWha, J.A. (1978) The metabolism and transport of abscisic acid during grain-fill in wheat. J. Exp. Bot. 29, 1299–1308

  2. Goldschmidt, E.D., Goren, R., Evan-Chen, Z., Bittner, S. (1973) Increase in free and bound ABA during natural and ethylene-induced senescence of citrus fruit peel. Plant Physiol. 51, 879–882

  3. Harrison, M.A., Walton, D.C. (1975) Abscisic acid metabolism in water-stressed bean leaves. Plant Physiol. 56, 250–254

  4. Koshimizu, K., Inui, M., Fukui, H., Mitsui, T. (1968) Isolation of [+]-abscisyl-β-D-glucopyranoside from immature fruit of Lupinus luteus. Agric. Biol. Chem. 32, 789–791

  5. Lehmann, H., Miersch, O., Schütte, H.R. (1975) Synthese von Abscisyl-β-D-glucopyranosid. Z. Chem. 15, 443

  6. Lowry, J.M., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurements with the Folin phenol reagent.

  7. Martin, G.C., Scott, I.M., Neill, S.J., Horgan, R. (1982) Identification of abscisic acid-glucose ester, indole-3-acetic acid, zeatin and zeatin riboside in receptacles of pear. Phytochemistry 21, 1079–1082

  8. Milborrow, B.V. (1970) The metabolism of abscisic acid. J. Exp. Bot. 21, 17–29

  9. Milborrow, B.V. (1978) The stability of conjugated abscisic acid during wilting. J. Exp. Bot. 29, 1059–1066

  10. Milborrow, B.V., Mallaby, R. (1975) Occurrence of methyl-(+)-abscisate as an artefact of extraction. J. Exp. Bot. 26, 741–748

  11. Neill, S.J., Horgan, R., Walton, D.C., Lee, T.S. (1982) The biosynthesis of abscisic acid in Cercospora rosicola. Phytochemistry 21, 61–65

  12. Pierce, M., Raschke, K. (1981) Synthesis and metabolism of abscisic acid in detached leaves of Phaseolus vulgaris L. after loss and recovery of turgor. Planta 153, 156–165

  13. Roberts, D.L., Heckmann, R.A., Hege, B.P., Bellin, S.A. (1968) Synthesis of (RS)-abscisic acid. J. Org. Chem. 33, 3566–3569

  14. Weiler, E.W. (1980) Radioimmunoassay for the differential and direct analysis of free and conjugated abscisic acid in plant extracts. Planta 148, 262–272

  15. Zeevaart, J.A.D. (1980) Changes in the levels of abscisic acid and its metabolites in excised leaf blades of Xanthium strumarium during and after water stress. Plant Physiol. 66, 672–678

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Neill, S.J., Horgan, R. & Heald, J.K. Determination of the levels of abscisic acid-glucose ester in plants. Planta 157, 371–375 (1983).

Download citation

Key words

  • Abscisic acid-glucose ester
  • Citrus (ABA ester)
  • Persea
  • Phaseolus (ABA ester)
  • Water stress