Planta

, Volume 166, Issue 2, pp 244–251 | Cite as

Transport of basic amino acids in Riccia fluitans: Evidence for a second binding site

  • E. Johannes
  • H. Felle
Article

Abstract

The transport of several amino acids with different side-chain characteristics has been investigated in the aquatic liverwort Riccia fluitans. i) The saturation of system I (neutral amino acids) by addition of excess α-aminoisobutyric acid to the external medium completely eliminated the electrical effects which are usually set off by neutral amino acids. Under these conditions arginine and lysine significantly depolarized the plasmalemma. ii) L- and D-lysine/arginine were discriminated against in favour of the L-isomers. iii) Increasing the external proton concentration in the interval pH 9 to 4.5 stimulated plasmalemma depolarization, electrical net current, and uptake of [14C]-basic amino acids. iv) Uptake of [14C]-glutamic acid took place only at acidic pHs. v) [14C]-histidine uptake had an optimum between pH 6 and 5.5. vi) Overlapping of the transport of basic, neutral, and acidic amino acids was common. It is suggested that besides system I, a second system (II), specific for basic amino acids, exists in the plasmalemma of Riccia fluitans. It is concluded that the amino-acid molecule with an uncharged side chain is the substrate for system I, which also binds and transports the neutral species of acidic amino acids, whereas system II is specific for amino acids with a positively charged side chain. The possibility of system II being a proton cotransport is discussed.

Key words

Amino acid (basic, transport) Bryophyta Membrane depolarization Proton cotransport Riccia 

Abbreviation

AiB

α-aminoisobutyric acid

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References

  1. Bertl, A., Felle, H., Bentrup, F.W. (1984) Amine transport in Riccia fluitans. Cytoplasmic and vacuolar pH recorded by a pH-sensitive microelectrode. Plant Physiol. 76, 75–78Google Scholar
  2. Cheung, Y.-N.S., Nobel, P.S. (1973) Amino acid uptake by pea leaf fragments. Specificity, energy sources, and mechanism. Plant Physiol. 52, 633–637Google Scholar
  3. Cole, K.S. (1968) Membrane, ions and impulses. pp. 152–168, University of California Press, BerkeleyGoogle Scholar
  4. Christensen, H.N., Handlogten, M.E. (1969) Reactions of neutral amino acids plus Na+ with a cationic amino acid transport system. FEBS Lett. 3, 14–17Google Scholar
  5. Felle, H. (1980) Amine transport at the plasmalemma of Riccia fluitans. Biochim. Biophys. Acta 602, 181–195Google Scholar
  6. Felle, H. (1981a) Sterospecificity and electrogenicity of amino acid transport in Riccia fluitans. Planta 152, 505–512Google Scholar
  7. Felle, H. (1981b) A study of the current-voltage relationships of electrogenic active and passive membrane elements in Riccia fluitans. Biochim. Biophys. Acta 646, 151–160Google Scholar
  8. Felle, H. (1983) Driving forces and current-voltage characteristics of amino acid transport in rhizoid cells of Riccia fluitans. Is the carrier negatively charged? Biochim. Biophys. Acta 772, 307–312Google Scholar
  9. Felle, H., Bentrup, F.W. (1980) Hexose transport and membrane depolarization in Riccia fluitans. Planta 147, 471–476Google Scholar
  10. Felle, H., Gogarten, J.P., Bentrup, F.W., (1983) Phlorizin inhibits hexose transport across the plasmalemma of Riccia fluitans. Planta 157, 267–270Google Scholar
  11. Felle, H., Lühring, H., Bentrup, F.W. (1979) Serine transport and membrane depolarization in the liverwort Riccia fluitans. Z. Naturforsch. Teil C 34, 1222–1223Google Scholar
  12. Hüsemann, W., Barz, W. (1977) Photoautotrophic growth and photosynthesis in cell suspension cultures from Chenopodium rubrum. Physiol. Plant. 40, 77–81Google Scholar
  13. Jung, K.-D., Lüttge, U. (1980) Amino acid uptake by Lemma gibba by a mechanism with affinity to neutral L- and D-amino acids. Planta 150, 230–235Google Scholar
  14. Kinraide, T.B., Etherton, B. (1980) Electrical evidence for different mechanisms of uptake for basic, neutral, and acidic amino acids in oat coleoptiles. Plant Physiol. 65, 1085–1089Google Scholar
  15. Kinraide, T.B., Newman, I.A., Etherton, B. (1984) A quantitative simulation model for H+-amino acid cotransport to interpret the effects of amino acids on membrane potential and extracellular pH. Plant Physiol. 76, 806–813Google Scholar
  16. Komor, E., Tanner, W. (1971) Characterization of the active hexose transport system of Chlorella vulgaris. Biochim. Biophys. Acta 241, 170–179Google Scholar
  17. Komor, E., Tanner, W. (1974) The hexose-proton cotransport system of Chlorella: pH-dependent change in Km values and translocation constants of the uptake system. J. Gen. Physiol. 64, 568–581Google Scholar
  18. Mitchell, P. (1963) Molecule, group and electron translocation through natural membranes. Biochem. Soc. Symp. 22, 142–168Google Scholar
  19. Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco cultures. Physiol. Plant. 15, 473–479Google Scholar
  20. Reinhold, L., Shtarkshall, R.A., Ganot, D. (1970) Transport of amino acids in barley leaf tissue. II. The kinetics of an unnatural analogue. J. Exp. Bot. 21, 926–932Google Scholar
  21. Sanders, D., Slayman, C.L., Pall, M.L. (1983) Stoichiometry of H+/amino acid cotransport in Neurospora crassa revealed by current-voltage analysis. Biochim. Biophys. Acta 735, 67–76Google Scholar
  22. Sanders, D., Hansen, U.-P., Gradmann, D., Slayman, C.L. (1984) Generalized kinetic analysis of selective ionic effects on Michaelis parameters. J. Membrane Biol. 77, 123–152Google Scholar
  23. Sauer, N. (1984) A general amino acid permease is inducible in Chlorella vulgaris. Planta 161, 425–431Google Scholar
  24. Steinmüller, F., Bentrup, F.W. (1981) Amino acid transport in photoautotrophic suspension cells of Chenopodium rubrum L.: Stereospecificity and interaction with potassium ions. Z. Pflanzenphysiol. 102, 353–361Google Scholar
  25. von Bel, J.E.A., van Erven, A. (1979) Potassium cotransport and antiport during the uptake of sucrose and glutamic acid from the xylem vessels. Plant Sci. Lett. 15, 285–291Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • E. Johannes
    • 1
  • H. Felle
    • 1
  1. 1.Botanisches Institut I der Justus Liebig UniversitätGiessenFederal Republic of Germany

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