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Concentration Requirement of glycine as nitrogen source for supporting effective growth of certain marine microplanktonic algae

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Abstract

Eighteen species of marine phytoplankton from 9 algal classes were tested under axenic-culture conditions for their capacity to improve phototrophic-growth performance on glycine with increasing concentration of this amino-acid serving as sole nitrogen source. Whereas all these species showed poor-to-no growth on 0.5 mM glycine, 14 species manifested considerable gain in growth-rate and yield at higher concentration levels. The highest level tested (25 mM) was generally not inhibitory and produced the best yields in these cases. Some species (notably Skeletonema costatum) showed marked reduction in the adaptation-lag period with increased glycine concentration, but Chlamydomonas palla required a very long adaptation period, which was little affected by concentration. Dunaliella tertiolecta, Emiliania huxleyi and Chroomonas salina showed sharp (albeit elevated) concentration thresholds for efficient glycine utilization. Rhodomonas lens and two cyanophycean strains, Agmenellum quadruplicatum and Anacystis marina, appeared incapable of successful growth on glycine at all concentrations tested, while the rhodophyte Porphyridium marinum displayed marginal growth. The results were insufficient to draw taxonomically general conclusions on algal-class potential for glycine utilization, but they showed unequivocally that more than 83% of phytoplankton species from 9 algal classes can utilize glycine for growth if the appropriate substrate concentration is provided.

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Communicated by J.M. Pérès, Marseille

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Berland, B.R., Bonin, D.J., Guérin-Ancey, O. et al. Concentration Requirement of glycine as nitrogen source for supporting effective growth of certain marine microplanktonic algae. Mar. Biol. 55, 83–92 (1979). https://doi.org/10.1007/BF00397303

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Keywords

  • Phytoplankton
  • Nitrogen Source
  • Chlamydomonas
  • Dunaliella
  • Skeletonema