Planta

, Volume 158, Issue 6, pp 482–486 | Cite as

The effect of some environmental factors on the production of L-DOPA by alginate-entrapped cells of Mucuna pruriens

  • Harm J. Wichers
  • Theo M. Malingré
  • Hindrik J. Huizing
Article

Abstract

In-vitro-grown cells of Mucuna pruriens, immobilized in calcium-alginate gels, were able to transform the precursor L-tyrosine into L-dihydroxyphenylalanine (L-DOPA). After the immobilization in alginate the plant cells released 90% of the produced L-DOPA into the medium; supplementation of the medium with calcium inhibited both the transformation of L-tyrosine into L-DOPA and the release of L-DOPA into the medium. Continuous illumination of the beads had a slight beneficial effect on the synthesis of L-DOPA. A simple production medium for the transformation of L-tyrosine into L-DOPA was designed. This medium contained only sucrose and sodium chloride as osmotic stabilizers, a low concentration of calcium chloride for stabilization of the alginate beads, and L-tyrosine as the precursor.

Key words

Alginate matrix Cell culture (immobilization) Dihydroxyphenylalanine Mucuna 

Abbreviations

DOPA

dihydroxyphenylalanine

MS-medium

Murashige, Skoog-medium

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References

  1. Brain, K.R. (1976) Accumulation of L-DOPA in cultures from Mucuna pruriens. Plant Sci. Lett. 7, 157–161Google Scholar
  2. Brodelius, P., Deus, B., Mosbach, K., Zenk, M.H. (1979) Immobilized plant cells for the production and transformation of natural products. FEBS Lett. 103, 93–97Google Scholar
  3. Brodelius, P., Nilsson, K. (1980) Entrapment of plant cells in different matrices. FEBS Lett. 122, 312–316Google Scholar
  4. Daxenbichler, M.E., Van Etten, C.H., Hallinan, E.A., Earle, F.R. (1971) Seeds as sources of L-DOPA. J. Med. Chem. 14, 463–465Google Scholar
  5. Felix, H., Brodelius, P., Mosbach, K. (1981) Enzyme activities of the primary and secondary metabolism of simultaneously permeabilized and immobilized plant cells. Anal. Biochem. 116, 462–470Google Scholar
  6. Griffith, T., Conn, E.E. (1973) Biosynthesis of 3,4-dihydroxyphenylalanine in Vicia faba. Phytochemistry 12, 1651–1656Google Scholar
  7. Morris, P., Fowler, M.W. (1981) A new method for the production of fine plant cell suspension cultures. Plant Cell Tissue Organ Culture 1, 15–24Google Scholar
  8. Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497Google Scholar
  9. Oosterhuis, B., Brunt, K., Westerink, B.H.C., Doornbos, D.A. (1980) Electrochemical detector flow cell based on a rotating disk elektrode for continuous flow analysis and high performance liquid chromatography of catecholamines. Anal. Chem. 52, 203–205Google Scholar
  10. Remmen, S.F.A., Ellis, B.E. (1980) DOPA synthesis in non-producer cultures of Mucuna deeringiana. Phytochemistry 19, 1421–1423Google Scholar
  11. Veliky, I.A., Jones, A. (1981) Bioconversion of gitoxigenin by immobilized plant cells in a column bioreactor. Biotechnol. Lett. 3, 551–555Google Scholar
  12. Widholm, J.M. (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47, 189–194Google Scholar
  13. Wykes, J.R., Dunnill, P., Lilly, M.D. (1971) Conversion of tyrosine to L-dihydroxyphenylalanine using immobilized tyrosinase. Nature (London) 230, 187Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Harm J. Wichers
    • 1
  • Theo M. Malingré
    • 1
  • Hindrik J. Huizing
    • 1
  1. 1.Laboratory of Pharmacognosy and Galenic PharmacyGroningenThe Netherlands

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