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The genome of B. subtilis phage SPP1

Physical arrangement of phage genes

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41 genes of SPP1 have been delineated by using complementation analyses of 75 conditionally lethal (ts and sus) mutations. The physical locations of these genes on the SPP1 chromosome have been determined by transfection/marker rescue experiments in which restriction endonuclease generated fragments of SPP1 DNA were used as donor DNA. The physical order of these fragments has been previously established (Ratcliff et al., 1979).

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Correspondence to T. A. Trautner.

Additional information

Part of this work is from the doctoral thesis submitted by M. Behncke to the Freie Universität Berlin (1973).

Communicated by E. Bautz

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Behrens, B., Lüder, G., Behncke, M. et al. The genome of B. subtilis phage SPP1. Molec. gen. Genet. 175, 351–357 (1979). https://doi.org/10.1007/BF00397235

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  • Restriction Endonuclease
  • Physical Location
  • Complementation Analysis
  • Physical Order
  • Rescue Experiment