Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The genome of B. subtilis phage SPP1

Physical arrangement of phage genes

  • 54 Accesses

  • 27 Citations

Summary

41 genes of SPP1 have been delineated by using complementation analyses of 75 conditionally lethal (ts and sus) mutations. The physical locations of these genes on the SPP1 chromosome have been determined by transfection/marker rescue experiments in which restriction endonuclease generated fragments of SPP1 DNA were used as donor DNA. The physical order of these fragments has been previously established (Ratcliff et al., 1979).

This is a preview of subscription content, log in to check access.

References

  1. Behncke, M.: Genkonversion bei dem B. subtilis Phagen SPP1. Untersuchungen von Heteroduplices aus komplementären DNA-Strängen mit Wildtyp- und konditional-lethalem Mutantentyp. Dissertation Freie Universität Berlin (1973)

  2. Biswal, N., Kleinschmidt, A.K., Spatz, H.Ch., Trautner, T.A.: Physical properties of the DNA of bacteriophage SP50. Mol. Gen. Genet. 100, 39–55 (1967)

  3. Burger, K.J., Trautner, T.A.: Specific labelling of replicating SPP1 DNA. Analysis of viral DNA synthesis and identification of phage dna-genes. Mol. Gen. Genet. 166, 277–285 (1978)

  4. Cerdá-Olmeda, E., Hanawalt, P.C., Guerola, N.: Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome. J. Mol. Biol. 33, 705–719 (1968)

  5. Cregg, J.M., Stewart, C.R.: Terminal redundancy of “high frequency of recombination” markers of Bacillus subtilis phage SP01. Virology 86, 530–541 (1978)

  6. Esche, H., Schweiger, M., Trautner, T.A.: Gene expression of bacteriophage SPP1. I. Phage directed protein synthesis. Mol. Gen. Genet. 142, 45–55 (1975)

  7. Georgopoulos, C.P.: Suppressor system in B. subtilis 168. J. Bacteriol. 97, 1397–1402 (1969)

  8. McIntosh, P., Dunker, R., Mulder, C., Brown, N.C.: DNA of Bacillus subtilis bacteriophage SPP1. Physical mapping and localization of the origin of replication. J. Virol. 28, 865–876 (1978)

  9. Mertens, G., Amann, E., Reeve, J.N.: Bacteriophage SPP1 polypeptides synthesized in infected minicells and in vitro. Molec. Gen. Genet. Mol. Gen. Genet. 172, 271–279 (1979)

  10. Montenegro, M.A., Esche, H., Trautner, T.A.: Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III. Mol. Gen. Genet. 149, 131–134 (1976)

  11. Morelli, G., Fisseau, C., Behrens, B., Trautner, T.A., Luh, J., Ratcliff, S.W., Allison, D.P., Ganesan, A.T.: The genome of B. subtilis phage SPP1: The topology of DNA molecules. Mol. Gen. Genet. 168, 153–164 (1979)

  12. Morelli, G., Montenegro, M.A., Hillenbrandt, G., Scherzinger, E., Trautner, T.A.: The genome of B. subtilis phage SPP1: Assignment of 5′-3′-polarity to the complementary strands of SPP1 DNA. Mol. Gen. Genet. 164, 93–97 (1978)

  13. Okubo, S., Yanagida, T.: Isolation of a suppressor mutant in B. subtilis. J. Bacteriol. 95, 1187–1188 (1968)

  14. Ratcliff, S.W., Luh, J., Ganesan, A.T., Behrens, B., Thompson, R., Montenegro, M.A., Morelli, G., Trautner, T.A.: The genome of Bacillus subtilis phage SPP1: The arrangement of restriction endonuclease generated fragments. Mol. Gen. Genet. 168, 165–172 (1979)

  15. Rottländer, E., Trautner, T.A.: Genetic and transfection studies with B. subtilis phage SP50. I. Phage mutants with restricted growth on B. subtilis strain 168. Mol. Gen. Genet. 108, 47–60 (1970)

  16. Spatz, H.Ch., Trautner, T.A.: One way to do experiments on gene conversion? Mol. Gen. Genet. 109, 84–106 (1970).

  17. Spatz, H.Ch., Trautner, T.A.: The role of recombination in transfection of B. subtilis. Mol. Gen. Genet. 113, 174–190 (1971)

  18. Thuring, R.W.G., Sanders, J.P.M., Borst, P.: A freeze squeeze method for recovering long DNA from agarose gels. Anal. Biochem. 66, 213–220 (1975)

  19. Trautner, T.A., Pawlek, B., Bron, S., Anagnostopoulos, C.: Restriction and Modification in B. subtilis. Biological Aspects. Mol. Gen. Genet. 131, 181–191 (1975)

Download references

Author information

Correspondence to T. A. Trautner.

Additional information

Part of this work is from the doctoral thesis submitted by M. Behncke to the Freie Universität Berlin (1973).

Communicated by E. Bautz

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Behrens, B., Lüder, G., Behncke, M. et al. The genome of B. subtilis phage SPP1. Molec. gen. Genet. 175, 351–357 (1979). https://doi.org/10.1007/BF00397235

Download citation

Keywords

  • Restriction Endonuclease
  • Physical Location
  • Complementation Analysis
  • Physical Order
  • Rescue Experiment