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Improved fluorescent microscopy for measuring the standing stock of phytoplankton including fragile components

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Fluorescent microscopy has been used to separate autotrophic from heterotrophic microorganisms in environmental studies. We report on an improvement on this method for measuring standing stock of phytoplankton. Our method employs a membrane filter and can measure numbers of living phytoplankton including fragile flagellates and size of phytoplankton cells. This method was successfully used in Tokyo Bay and Kuroshio regions.

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Correspondence to T. Tsuji.

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Communicated by M. Anraku, Hiroshima

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Tsuji, T., Yanagita, T. Improved fluorescent microscopy for measuring the standing stock of phytoplankton including fragile components. Mar. Biol. 64, 207–211 (1981).

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  • Microscopy
  • Phytoplankton
  • Fluorescent Microscopy
  • Membrane Filter
  • Environmental Study