Human Genetics

, Volume 43, Issue 1, pp 47–52 | Cite as

Replication of chromosomal DNA in cultured abnormal human cells

  • Yu. B. Yurov
Original Investigations


The replication of chromosomal DNA in a series of abnormal human cell cultures has been studied by means of DNA-fiber autoradiography. In lymphocytes with trisomy 21, in fibroblasts of 45,X; 47,XXX; 49,XXXXY; and 49,XXXXX chromosomal constitution, and in fibroblasts from a patient with xeroderma pigmentosum (De Sanctis-Cacchione syndrome), the rate of DNA replication does not differ from that in normal cells, varying in a single fork from 0.2 to 1.0 μm/min with a mean of about 0.6 μm/min. In fibroblasts with trisomy 7 the rate of DNA replication is greater, varying from 0.3 to 1.2 μm/min with a mean of about 0.8 μm/min. The sizes of replication units in all cells examined are from 80 to 500 μm with a mean of about 200–300 μm.


Cell Culture Internal Medicine Metabolic Disease Normal Cell Human Cell 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Yu. B. Yurov
    • 1
  1. 1.Institute of Medical GeneticsAcademy of Medical Sciences of the USSRMoscowUSSR

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