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Human Genetics

, Volume 43, Issue 1, pp 47–52 | Cite as

Replication of chromosomal DNA in cultured abnormal human cells

  • Yu. B. Yurov
Original Investigations

Summary

The replication of chromosomal DNA in a series of abnormal human cell cultures has been studied by means of DNA-fiber autoradiography. In lymphocytes with trisomy 21, in fibroblasts of 45,X; 47,XXX; 49,XXXXY; and 49,XXXXX chromosomal constitution, and in fibroblasts from a patient with xeroderma pigmentosum (De Sanctis-Cacchione syndrome), the rate of DNA replication does not differ from that in normal cells, varying in a single fork from 0.2 to 1.0 μm/min with a mean of about 0.6 μm/min. In fibroblasts with trisomy 7 the rate of DNA replication is greater, varying from 0.3 to 1.2 μm/min with a mean of about 0.8 μm/min. The sizes of replication units in all cells examined are from 80 to 500 μm with a mean of about 200–300 μm.

Keywords

Cell Culture Internal Medicine Metabolic Disease Normal Cell Human Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Yu. B. Yurov
    • 1
  1. 1.Institute of Medical GeneticsAcademy of Medical Sciences of the USSRMoscowUSSR

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