Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Morphology, nutrition and taxonomy of an Aplanochytrium sp. from the Sargasso Sea

  • 143 Accesses

  • 9 Citations

Abstract

Aplanochytrium sp. 2400/57 was isolated from samples of sargasso weed collected in the Sargasso Sea on April 4, 1979. The monocentric eucarpic thallus of this organism produced aplanospores by successive bipartitioning. The cell wall contained only 20% polysaccharide, composed of fucose, galactose, xylose, glucose, and galacturonic acid. The guanine+cytosine content of the DNA (mol% G+C) was 37.6%. L-isoleucine, L-leucine, and L-proline were utilized as nitrogen sources in synthetic media of defined composition.

This is a preview of subscription content, log in to check access.

Literature cited

  1. Alderman, D. J., J. L. Harrison, G. B. Bremer and E. B. G. Jones: Taxonomic revisions in the marine biflagellate fungi: the ultrastructural evidence. Mar. Biol. 25, 345–357 (1974)

  2. Bahnweg, G.: Studies on the physiology of Thraustochytriales. I. Growth requirements and nitrogen nutrition of Thraustochytrium spp., Schizochytrium sp., Japonochytrium sp., Ulkenia spp., and Labyrinthuloides spp. Veröff. Inst. Meeresforsch. Bremerh. 17, 245–268 (1979)

  3. Bahnweg, G. and F. K. Sparrow: Aplanochytrium kerguelensis gen. nov. spec. nov., a new phycomycete from subantarctic marine waters. Arch. Mikrobiol. 81, 45–49 (1972)

  4. Bahnweg, G. and F. K. Sparrow: Four new species of Thraustochytrium from antarctic regions, with notes on the distribution of zoosporic fungi in the antarctic marine ecosystems. Am. J. Bot. 61, 754–766 (1974a)

  5. Bahnweg, G. and F. K. Sparrow: Occurrence, distribution and kinds of zoosporic fungi in subantarctic and antarctic waters. Veröff. Inst. Meeresforsch. Bremerh. Suppl. 5, 149–157 (1974b)

  6. Darley, W. M., D. Porter and M. S. Fuller: Cell wall composition and synthesis via Golgi-directed scale formation in the marine eucaryote, Schizochytrium aggregatum, with a note on Thraustochytrium sp. Arch. Mikrobiol. 90, 89–106 (1973)

  7. Dubois, M., K. A. Gillis, J. K. Hamilton, P. A. Rebers and F. Smith: Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)

  8. Dykstra, M. and D. Porter: Diplophrys marina, a new scale-forming marine protist with labyrinthulid affinities. Mycologia 76, 626–632 (1984)

  9. Gillis, M., J. De Ley and M. De Cleene: The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12, 143–153 (1970)

  10. Goldstein, S. and L. Moriber: Biology of a problematic marine fungus, Dermocystidium sp. I. Development and cytology. Arch. Mikrobiol 53, 1–11 (1966)

  11. Heunert, H. H.: Präparationsmethoden für Vitalbeobachtungen an Mikroorganismen. I. Kulturen in flüssigen Medien: Tauchkappen, Plattenkammer, Deckglaspräparate, Paraffinkammern. Mikrokosmos 63, 218–222 (1974)

  12. Krebs, K. G., D. Heusser and H. Wimmer: Sprühreagentien. In: Dünnschichtchromatographie (Hrsg. E. Stahl), pp 813–861. Berlin, Heidelberg: Springer Verlag 1967

  13. Lowry, O. H., N. J. Rosebrough, A. L. Farry and R. J. Randall: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)

  14. Mandel, M. and J. Marmur: Use of ultraviolet abosrbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology 12, 195–206 (1968)

  15. Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. mol. Biol. 3, 208–218 (1961)

  16. E. Merck AG: Die Untersuchung von Wasser, 9. Aufl., 224 pp. Darmstadt: E. Merck 1975

  17. Mitchell, A. D. and I. E. P. Taylor: Cell-wall proteins of Aspergillus niger and Chaetomium globosum. J. gen. Microbiol. 59, 103–109 (1969)

  18. Perkins, F. O.: Observations of thraustochytriaceaous (Phycomycetes) and labyrinthulid (Rhizopodea) ectoplasmatic nets on natural and artificial substrates — an electron microscope study. Can. J. Bot. 51, 485–491 (1973a)

  19. Perkins, F. O.: A new species of marine labyrinthulid Labyrinthuloides yorkensis gen. nov. spec. nov. — cytology and fine structure. Arch. Mikrobiol. 90, 1–17 (1973b)

  20. Ulken, A.: Phycomycetenfunde in der Sargassosee. Veröff. Inst. Meeresforsch. Bremerh. 18, 21–27 (1979)

  21. Wagner, H.: Beitrag zur Mikrobestimmung des Schwefels in organischen Substanzen. Mikrochim. Acta 1, 19–23 (1957)

Download references

Author information

Additional information

Communicated by O. Kinne, Oldendorf

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ulken, A., Jäckle, I. & Bahnweg, G. Morphology, nutrition and taxonomy of an Aplanochytrium sp. from the Sargasso Sea. Mar. Biol. 85, 89–95 (1985). https://doi.org/10.1007/BF00396419

Download citation

Keywords

  • Nitrogen
  • Glucose
  • Cell Wall
  • Polysaccharide
  • Xylose