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Sugar compartmentation in frost-hardy and partially dehardened cabbage leaf cells

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In frost-hardy and partially dehardened leaves of Brassica oleracea L. var. sabellica L. the distribution of cryoprotective sugars and of chloride between chloroplasts and the nonchloroplast part of leaf cells was investigated using the nonaqueous isolation technique as a means of cell fractionation. In chloroplasts of frost-hardy leaves high concentrations of sucrose and raffinose and comparatively low concentrations of chloride have been found. The ratios between sugars and chloride were so as to ascertain complete protection of the frost-sensitive thylakoid membranes during freezing. During dehardening, sugars decreased especially in the chloroplasts. There was a conversion of sucrose and raffinose into monosaccharides. This led to a large increase in the concentration of glucose and fructose in the nonchloroplast parts of the cells. There is evidence that the sugar concentration in the vacuole increased at the expense of sugars located in chloroplasts and cytoplasm. The quantity of sugars that remained in the chloroplasts did not appear to be sufficient for complete membrane protection at very low freezing temperatures.

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  1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulearis. Plant Physiol. 24, 1–15 (1949)

  2. Bergmeyer, H.U. (ed.) Methoden der enzymatischen Analyse. 3rd Ed., Weinheim.: Verlag Chemie 1974

  3. Duane, W.C., Krogmann, D.W.: Chloroplast storage with retention of photosynthetic activities. Biochim. biophys. Acta 71, 195–196 (1963)

  4. Heber, U.: Beziehungen zwischen der Größe von Chloroplasten und ihrem Gehalt an löslichen Eiweißen und Zuckern im Zusammenhang mit dem Frostresistenzproblem. Protoplasma 51, 284–298 (1959)

  5. Heber, U., Kirk, M.R., Gimmler, H., Schäfer, G.: Uptake and reduction of glycerate by isolated chloroplasts. Planta (Berl.) 120, 31–46 (1974)

  6. Heber, U., Pon, N.G., Heber, M.: Localization of carboxidismutase and triosephosphate dehydrogenase in chloroplasts. Plant Physiol. 38, 355–360 (1963)

  7. Heber, U., Santarius, K.A.: Loss of adenosine triphosphate synthesis caused by freezing and its relationship to frost hardiness problems. Plant Physiol. 39, 712–719 (1964)

  8. Heber, U., Santarius, K.A.: Water stress during freezing. In: Water and plant life. Ecological studies. Analysis and synthesis, Vol. 19, pp. 253–267, Lange, O.L., Kappen, L., Schulze, E.-D., eds. Berlin-Heidelberg-New York: Springer 1976

  9. Heber, U., Tyankova, L., Santarius, K.A.: Stabilization and inactivation of biological membranes during freezing in the presence of amino acids. Biochim. biophys. Acta 241, 578–592 (1971)

  10. Heber, U., Willenbrink, J.: Sites of synthesis and transport of photosynthetic products within the leaf cell. Biochim. biophys. Acta 82, 313–324 (1964)

  11. Heldt, H.W., Werdan, K., Gfeller, G., Milovancev, M.: Alkalization of the chloroplast stroma caused by light dependent proton flux into the thylakoid space. Biochim. biophys. Acta 314, 224–241 (1973)

  12. Jagendorf, A.T., Avron, M.: Cofactors and rates of photosynthetic phosphorylation by spinach chloroplasts. J. biol. Chem. 231, 277–290 (1958)

  13. Kappen, L., Ullrich, W.R.: Verteilung von Chlorid und Zuckern in Blattzellen halophiler Pflanzen bei verschieden hoher Frostresistenz. Ber. Dtsch. Bot. Ges. 83, 265–275 (1970)

  14. Klevstrand, R., Nordal, A.: A spraying reagent for paper chromatograms which is apparently specific for ketoheptoses. Acta Chem. Scand. 4, 1320 (1950)

  15. Morris, D.L.: The quantitative determination of carbohydrates with Dreywood's anthrone reagent. Science 107, 254–255 (1948)

  16. Partridge, S. M.: Anilin hydrogen phthalate as a spraying reagent for chromatography of sugars. Nature 164, 443 (1949)

  17. Santarius, K.A.: Der Einfluß von Elektrolyten auf Chloroplasten beim Gefrieren und Trocknen. Planta (Berl.) 89, 23–46 (1969)

  18. Santarius, K.A.: The effect of freezing on thylakoid membranes in the presence of organic acids. Plant Physiol. 48, 156–162 (1971)

  19. Santarius, K.A.: The protective effect of sugars on chloroplast membranes during temperature and water stress and its relationship to frost, desiccation and heat resistance. Planta (Berl.) 113, 105–114 (1973)

  20. Santarius, K.A., Stocking, C.R.: Intracellular localization of enzymes in leaves and chloroplast membrane permeability to compounds involved in amino acid synthesis. Z. Naturforsch. 24b, 1170–1179 (1969)

  21. Urbach, W., Hudson, M.A., Ullrich, W., Santarius, K.A., Heber, U.: Vertellung und Wanderung von Phosphoglycerat zwischen den Chloroplasten und dem Cytoplasma während der Photosynthese. Z. Naturforsch. 20b, 890–898 (1965)

  22. Volger, H.G., Heber, U.: Cryoprotective leaf proteins. Biochim. biophys. Acta 412, 335–349 (1975)

  23. Walter, H.: Die kryoskopische Bestimmung des osmotischen Wertes bei Pflanzen. In: Abderhaldens Handbuch der biologischen Arbeitsmethoden, Sect. XI, Part 4,1, pp. 353–371. Berlin-Wien: Urban and Schwarzenberg 1931

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Santarius, K.A., Milde, H. Sugar compartmentation in frost-hardy and partially dehardened cabbage leaf cells. Planta 136, 163–166 (1977). https://doi.org/10.1007/BF00396193

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Key words

  • Brassica
  • Chloroplasts
  • Frost hardiness
  • Nonaqueous cell fractionation
  • Sugar compartmentation