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Gibberellic acid and the fine structure of barley aleurone cells

II. Changes during the synthesis and secretion of α-amylase

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This paper describes the ultrastructural changes in barley aleurone cells following exposure to gibberollic acid (GA3) for 10–12 hr and longer. These changes involve a further proliferation of the endoplasmic reticulum (ER), distention of the endoplasmic reticulum (ER) cisternae (12–16 hr of GA3) and proliferation of vesicles from the ER and dictyosomes (14–22 hr). Accompanying these changes is a reduction in the size of the aleurone grains and a decrease in the number of spherosomes. Plastids and microbodies however appear to increase in number during this period of GA3 treatment. The relevance of these ultrastructural changes to GA3-stimulated synthesis of hydrolases is discussed.

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  1. Beevers, L.: Effect of gibberellic acid on the senescence of leaf discs of nasturtium (Tropaeolum majus). Plant Physiol. 41, 1074–1076 (1966).

  2. Briggs, D. E.: Biochemistry of barley germination: Action of gibberellic acid on barley endosperm. J. Inst. Brewing 69, 13–19 (1963).

  3. Chrispeels, M. J., and J. E. Varner: Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42, 398–406 (1967a).

  4. Filner, P., and J. E. Varner: A simple and unequivocal test for de novo synthesis of enzymes: density labeling of barley α-amylase with H2O18. Proc. nat. Acad. Sci. (Wash.) 58, 1520–1526 (1967).

  5. Hokin, L. E., and M. R. Hokin: The synthesis and secretion of digestive enzymes by pancreas tissue in vitro. In: The exocrine pancreas (A. V. S. de Reuck and M. P. Cameron, eds.), p. 186–207. Boston: Little, Brown 1962.

  6. Jacobsen, J. V., and J. E. Varner: Gibberellic acid-induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol. 42, 1596–1600 (1967).

  7. Jones, R. L.: The fine structure of barley aleurone cells. Planta (Berl.) 85, 359–375 (1969a).

  8. —: Gibberellic acid and the fine structure of barley aleurone cells. I. Changes during the lag phase of α-amylase synthesis. Planta (Berl.) 87, 119–133 (1969b).

  9. —, and J. E. Varner: The bioassay of gibberellins. Planta (Berl.) 72, 53–59 (1967).

  10. Newcomb, E. H.: A spiny vesicle in slime-producing cells of the bean root. J. Cell Biol. 35, C17–C22 (1967).

  11. Paleg, L.: Physiological effects of gibberellic acid. II. On starch hydrolyzing enzymes of barley endosperm. Plant Physiol. 35, 902–906 (1960).

  12. Varner, J. E.: Gibberellic acid controlled synthesis of α-amylase in barley endosperm. Plant Physiol. 39, 413–415 (1964).

  13. —: Seed development and germination. In: Plant biochemistry (J. Bonner and J. E. Varner, eds.), p. 763–792. New York: Acad. Press 1965.

  14. Yomo, H.: Studies on the α-amylase activating substance. IV. On the amylase activating action of gibberellin. Hakko Kyokai-shi 18, 600–602 (1960).

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The skillful technical assistance of Mrs. Janet Price is gratefully acknowledged. Supported by National Science Foundation grant GB-8332.

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Jones, R.L. Gibberellic acid and the fine structure of barley aleurone cells. Planta 88, 73–86 (1969). https://doi.org/10.1007/BF00396117

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  • Endoplasmic Reticulum
  • Fine Structure
  • Hydrolase
  • Gibberellic Acid
  • Ultrastructural Change