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A comparison of methods used for testing staphylokinase (fibrinolysin) production in Staphylococcus strains

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Different nutrient bases, fibrinogen or heated plasma preparations and plasminogen sources were compared in tests for staphylokinase production by Staphylococcus aureus and other staphylococcal species. Media containing a nutrient base, bovine fibrin and dog plasma or serum proved to be more sensitive than the other substrates or combinations of substrates tested. Comparative testing on plates with and without dog plasminogen or with plasmin inhibitors, was found to be nescessary for the differentiation of staphylokinase from protease effects. Staphylokinases were produced by S. aureus, S. hyicus, S. simulans, S. sciuri and S. xylosus strains.

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  1. BrakmanP. and AstrupT. 1971. The fibrin plate method for assay of fibrinolytic agents. p. 332–336. In N. V.Bang (ed.) Thrombosis and bleeding disorders. Theory and practice. — Academic Press. New York and London.

  2. ChristieR., GraydonJ. J. and WoodsE. F. 1945. Staphylococcal fibrinolysin — Aust. J. Exp. Biol. Med. Sci. 23:127–130.

  3. ChristieR. and WilsonH. 1941. A test of staphylococcal fibrinolysis. — Aust. J. Exp. Biol. Med. Sci. 19:329–332.

  4. DevrieseL. A., DevosA. H., BeumerJ. and MaesR. 1972. Characterization of staphylococci isolated from poultry. — Poultry Sci. 51:389–397.

  5. DevrieseL. A., HájekV., OedingP., MeyerS. A. and SchleiferK. H. 1978. Staphylococcus hyicus (Sompolinsky 1953) comb. nov. and S. hyicus subsp. chromogenes subsp. nov. — Int. J. Syst. Bacteriol. 24:482–490.

  6. ElekS. D. (ed.). 1959. p. 279–286. In: Staphylococcus pyogenes and its relation to disease. — E. and S. Livingstone Ltd. London and Edinburgh.

  7. GerheimE. B. and FergusonJ. H. 1949. Species reactivity to staphylokinase. — Proc. Soc. Exp. Biol. Med. 71: 261–263.

  8. HájekV. and MaršálekE. 1971. The differentiation of pathogenic staphylococci and a suggestion for their classification. — Zentralbl. Bakteriol., Parasitenkd., Infectionskr. Hyg. Abt. 1. Orig. Reihe A 217:176–182.

  9. HentschelG. und BlobelH. 1968. Untersuchung über Staphylokokken-Fibrinolysin. — Zentralbl. Bakteriol., Parasitenkd., Infectionskr. Hyg. Abt. 1. Orig. 208:193–201.

  10. HintonN. A. and OrrJ. H. 1957. The distribution of toxins in coagulase-positive staphylococci isolated from infections and carriers. — J. Lab. Clin. Med. 50:901–912.

  11. HutchisonJ. G. P. 1962. Müller's phenomenon and its relationship to plasminogen activation and to blood constituents producing opacity in an agar gel. — J. Pathol. Bacteriol. 84:289–306.

  12. KondoI., FujiseK. and SakuraiS. 1976. Staphylokinase mediated by lysogenic conversion. p. 529–538. In J.Jeljaszewicz (ed.), Staphylococci and staphylococcal diseases. — Zentralbl. Bakteriol., Parasitenkd., Infectionskr. Hyg. Abt. 1. Suppl. 5.

  13. LackC. H. 1948. Staphylokinase: an activator of plasma protease. — Nature 161:559–560.

  14. LackC. H. and GlanvilleK. L. A. 1970. Staphylokinase. p. 706–714. In G. E.Perlman and L.Lorand (eds), Methods in enzymology Vol. XIX. — Academic Press, New York.

  15. MadisonR. R. and DartE. E. 1936. Veterinary staphylolysin. — Proc. Soc. Exp. Biol. Med. 34:299–300.

  16. MeyerW. 1966. Differenzierungschema für Standortvarianten von Staphylococcus aureus. —Zentralbl. Bakteriol., Parasitenkd., Infectionskr. Hyg. Abt. I. Orig. 201:465–481.

  17. QuieP. G. and WannamakerL. W. 1961. Staphylococcal Müller phenomenon: relationship to the plasminogen-plasmin system. — J. Bacteriol. 82: 770–783.

  18. SweetB., McNicolG. P. and DouglasA. S. 1965. In vitro studies on staphylokinase. — Clin. Sci. 29:375–382.

  19. WinklerK. C., deWaartJ., GrootsenC., ZegersB. J. M., TellierN. F. and VertregtC. D. 1965. Lysogenic conversion of staphylococci to loss of β-toxin. — J. Gen. Microbiol. 39:321–333.

  20. WitteW., HummelR., MeyerW., ExnerH. and WundrakR. 1977. Ecology of Staphylococcus aureus: characterization of strains from chickens. — Z. Allg. Mikrobiol. 17: 639–646.

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Devriese, L.A., Van De Kerckhove, A. A comparison of methods used for testing staphylokinase (fibrinolysin) production in Staphylococcus strains. Antonie van Leeuwenhoek 46, 457–465 (1980). https://doi.org/10.1007/BF00395826

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  • Fibrinogen
  • Staphylococcus Aureus
  • Plasminogen
  • Comparative Testing
  • Plasmin