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Planta

, Volume 161, Issue 2, pp 142–147 | Cite as

Nitrogen nutrition and the accumulation of free and sinapoyl-bound malic acid in Raphanus sativus cotyledons

  • Brigitte Dahlbender
  • Dieter Strack
Article

Abstract

Seedlings of red radish (Raphanus sativus L. var. sativus) accumulated high amounts of free malic acid and sinapoylmalate, when grown on nitrate as the sole N-source. In the presence of ammonium (NO 3 : NH 4 + , 1:2) both metabolites failed to accumulate, and the levels of arginine, asparagine, glutamine, histidine, and serine were greatly increased. The extractable activity of 1-sinapoylglucose: l-malate sinapoyltransferase, an enzyme which plays a key role in channelling malic acid into the sinapic-acid metabolism of this plant, was positively correlated with the malic-acid level in cotyledons. The possibility is discussed that free malic acid might be the likely candidate for regulating the activity of 1-sinapoylglucose: l-malate sinapoyltransferase.

Key words

Malic acid Nitrogen nutrition Raphanus 1-Sinapoylglucose l-malate sinapoyl-transferase (control) 

Abbreviation

SMT

sinapoylglucose: L-malate sinapoyltransferase

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Brigitte Dahlbender
    • 1
  • Dieter Strack
    • 1
  1. 1.Botanisches Institut der Universität KölnKöln 41Federal Republic of Germany

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