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Gibberellic-acid-responsive protoplasts from mature aleurone of Himalaya barley


Gibberellic acid (GA3)-responsive protoplasts were prepared from mature aleurone layers of Himalaya barley. Protoplasts prepared in air (air-protoplasts) synthesized α-amylase (EC in the presence of GA3 at a rate which was 4–5 times greater that in its absence. Protoplasts prepared in nitrogen (N2-protoplasts) took longer than air-protoplasts to respond to GA3 but α-amylase synthesis ultimately attained a rate which was similar to that for air-protoplasts and which was many times that occurring in the absence of the hormone. Many characteristics of the protoplast response were similar to those of intact aleurone layers. α-Amylase arose by new synthesis, its synthesis was inhibited by abscisic acid, it was isozymically similar to aleurone layer enzyme, most of it was secreted into the incubation medium and its synthesis was accompanied by accumulation of α-amylase mRNA. GA3-induced changes in protein synthesis and cell structure also resembled those of intact aleurone cells. We conclude that the response of the protoplasts to GA3 is normal and that they present a useful system for the study of GA3 action in barley aleurone.

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GA3 :

gibberellic acid


  1. Abdul-Baki, A.A. (1974) Pitfalls in using sodium hypochlorite as a seed disinfectant in 14C-incorporation studies. Plant Physiol. 53, 768–771

  2. Ashford, A.E., Jacobsen, J.V. (1974) Cytochemical localization of phosphatase in barley aleurone cells. The pathway of gibberellic-acid-induced enzyme release. Planta 120, 81–105

  3. Chandler, P.M., Higgins, T.J.V., Randall, P.J., Spencer, D. (1983) Regulation of legumin levels in developing pea seeds under conditions of sulfur deficiency. Plant Physiol. 71, 47–54

  4. Chandler, P.M., Zwar, J.A., Jacobsen, J.V., Higgins, T.J.V., Inglis, A.S. (1984) The effects of gibberellic acid and abscisic acid on α-amylase mRNA levels in barley aleurone layers: studies using an α-amylase cDNA clone. Plant Molec. Biol. (in press)

  5. Chrispeels, M.J., Varner, J.E. (1967) Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42, 398–406

  6. Eastwood, D. (1977) Responses of enzymically isolated aleurone cells of oat to gibberellin A3. Plant Physiol. 60, 457–459

  7. Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158

  8. Hanson, A.D., Jacobsen, J.V. (1984) Control of lactate dehydrogenase, lactate glycolysis and α-amylase by O2-deficit in barley aleurone layers. Plant Physiol. (in press)

  9. Higgins, T.J.V., Jacobsen, J.V., Zwar, J.A. (1982) Gibberellic acid and abscisic acid modulate protein synthesis and mRNA levels in barley aleurone layers. Plant Molec. Biol. 1, 191–215

  10. Hooley, R. (1982) Protoplasts isolated from aleurone layers of wild oat (Avena fatua L.) exhibit the classic response to gibberellic acid. Planta 154, 29–40

  11. Jacobsen, J.V. (1984) Regulation of protein synthesis in aleurone cells by gibberellin and abscisic acid. In: The Biochemistry and Physiology of Gibberellins Vol. 2, pp. 159–187, Crozier, A., ed. Praeger, New York

  12. Jacobsen, J.V., Knox, R.B. (1973) Cytochemical localization and antigenicity of α-amylase in barley aleurone tissue. Planta 112, 213–224

  13. Jacobsen, J.V., Scandalios, J.G., Varner, J.E. (1970) Multiple forms of amylase induced by gibberellic acid in isolated barley aleurone layers. Plant Physiol. 45, 367–371

  14. Jones, R.L. (1969a) Gibberellic acid and fine structure of barley aleurone cells. I. Changes during the lag-phase of α-amylase synthesis. Planta 87, 119–133

  15. Jones, R.L. (1969b) Gibberellic acid and the fine structure of barley aleurone cells. II. Changes during the synthesis and secretion of α-amylase. Planta 88, 73–86

  16. Jones, R.L., Armstrong, J.E., Taiz, L. (1973) Barley aleurone protoplasts: some observations on their structure and physiology. In: Protoplastes et fusion de cellules somatique végétales, pp. 109–117, Tempe, I., ed. Inst. Nat. Rech. Agron, Paris

  17. Jones, R.L., Price, J.M. (1970) Gibberellic acid and the fine structure of barley aleurone cells. III. Vacuolation of the aleurone cell during the phase of ribonuclease release. Planta 94, 191–202

  18. Nagata, T., Takebe, I. (1970) Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta 92, 301–308

  19. Taiz, L., Jones, R.L. (1971) The isolation of barley-aleurone protoplasts. Planta 101, 95–101

  20. Varner, J.E., Mense, R.M. (1972) Characteristics of the process of enzyme release from secretary plant cells. Plant Physiol. 49, 187–189

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Jacobsen, J.V., Zwar, J.A. & Chandler, P.M. Gibberellic-acid-responsive protoplasts from mature aleurone of Himalaya barley. Planta 163, 430–438 (1985).

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Key words

  • Aleurone protoplasts
  • α-Amylase
  • Anaerobiosis
  • Barley
  • Gibberellin Hordeum (aleurone)
  • Protoplast (aleurone)