Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Translation and transcription in imbibed and germinating spores of Anemia phyllitidis L.Sw.

  • 27 Accesses

  • 11 Citations

Abstract

Induction of germination by gibberellin or light is not a prerequisite for protein and RNA synthesis in spores of A. phyllitidis. Imbibed but non-induced spores of this fern show a high rate of translation as well as appreciably transcription of all RNA types. The pattern of in-vitro translation of polyadenylated RNA present in the dry spore corresponds with the in-vivo translation products under non-inductive conditions. New proteins are not detectable among the in-vitro and in-vivo translation products until 48 h after a germination stimulus. Although the dark imbibition of spores is accompanied by a reduction in the lengths of polyadenylated tracts of the stored mRNA, this fraction is characterized by a remarkable stability which is undoubtedly a prerequisite for the high viability of these fern spores. The importance of these results for the general meaning of stored mRNA in spore and seed germination processes is discussed.

This is a preview of subscription content, log in to check access.

Abbreviations

GA3 :

gibberellic acid

poly(A)+RNA:

polyadenylated RNA

SDS:

sodium dodecyl sulfate

References

  1. Aviv, H., Leder, P. (1972) Purification of biologically active globin m-RNA by chromatography on oligothymidylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69, 1408–1412

  2. Bhat, S.P., Padayatty, J.D. (1974) Presence of conserved messenger RNA in rice embryos. Ind. J. Biochem. Biophys. 11, 47–50

  3. Caers, L.I., Peumans, W.J., Carlier, A.R. (1979) Preformed and newly synthesized messenger RNA in germinating wheat embryos. Planta 144, 491–496

  4. Chen, D., Sarid, S., Katchalski, E. (1968) Studies on the nature of messenger RNA in germinating wheat embryos. Proc. Natl. Acad. Sci. USA 60, 902–909

  5. Delseny, M., Aspart, L., Cooke, R. (1980/81) Studies on ribonucleic acids during radish seed germination: a search for potential regulation mechanisms. Israel J. Bot. 29, 246–258

  6. Dyer, A.F. (1979) The culture of fern gametophytes for experimental investigation. In: The experimental biology of ferns, pp. 253–305, Dyer, A.F., ed. Academic Press, London New York San Francisco

  7. Dyer, A.F., Cran, D.G. (1976) The formation and ultrastructure of rhizoids on protonema of Dryopteris borrei Newm. Ann. Bot. 40, 757–765

  8. Fechner, A., Schraudolf, H. (1982) Isolation and characterization of poly (adenylic acid)-containing ribonucleic acid from dry spores of Anemia phyllitidis L.Sw. Z. Pflanzenphysiol. 108, 419–428

  9. Fleck, J., Durr, A., Fritsch, C., Lett, M.C., Hirth, L. (1980) Comparison of proteins synthesized in vivo and in vitro by mRNA from isolated protoplasts. Planta 148, 453–454

  10. Fountain, D.W., Bewley, I.D. (1973) Polyribosome formation and protein synthesis in imbibed but dormant lettuce seeds. Plant Physiol. 52, 604–607

  11. Gemmrich, A.R. (1977) Mobilization of reserve lipids in germinating spores of the fern Anemia phyllitidis L.. Plant Sci. Lett. 9, 301–307

  12. Gordon, M.E., Payne, P.I. (1976) In vitro translation of the long-lived messenger ribonucleic acid of dry seeds. Planta 130, 269–273

  13. Greene, F.C. (1983) Expression of storage protein genes in developing wheat (Triticum aestium L.) seeds. Correlation of RNA accumulation and protein synthesis. Plant Physiol. 71, 40–46

  14. Jachymczyk, W.J., Sieliwanowicz, B., Chlebowicz, E. (1974) Activation of preexisting messenger RNA in dry pea embryonic axes. Acta Biochim. Pol. 21, 137–143

  15. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature (London) 227, 680–685

  16. Mitchell, R.C., Villiers, T.A. (1972) Polysome formation in light-controlled dormancy. Plant Physiol. 50, 671–674

  17. Mohr, H. (1956) Die Abhängigkeit des Protonemawachstums und der Protonemapolarität bei Farnen vom Licht. Planta 47, 127–158

  18. Mori, T., Wakabayashi, Y., Takagi, S. (1978) Occurance of m-RNA of storage protein in dry soybean seeds. J. Biochem. (Tokyo) 84, 1103–1111

  19. Naef, U. (1966) On dark germination and antheridium formation in Anemia phyllitidis. Physiol. Plant. 19, 1079–1088

  20. Peumans, W.J., Delaey, B.M., Manickham, A., Carlier, A.R. (1980) Efficient translation of long lived messengers in extracts from dry pea primary axes. Evidence for the presence of lectin mRNA. Planta 150, 286–290

  21. Raghavan, V. (1971) Synthesis of protein and RNA for initiation and growth of the protonema during germination of braken fern spore. Exp. Cell Res. 65, 401–407

  22. Raghavan, V. (1976) Gibberellic acid-induced germination of spores of Anemia phyllitidis: nucleic acid and protein synthesis during germination. Am. J. Bot. 63, 960–972

  23. Raghavan, V. (1977 a) Cell morphogenesis and macromolecule synthesis during phytochrome-controlled germination of spores of the fern Pteris vittata. J. Exp. Bot. 28, 439–446

  24. Raghavan, V. (1977 b) Gibberellic acid-induced germination of Anemia phyllitidis: autoradiographic study of the timing and regulation of nucleic acid and protein synthesis in relation to cell morphogenesis. J. Cell Sci. 23, 85–100

  25. Reger, B.J., Egley, G.H., Swanson, Ch.R. (1975) Polysome formation in light-sensitive common purslane seeds. Plant Physiol. 55, 928–931

  26. Schraudolf, H. (1962) Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien I. Auslösung der Antheridienbildung und Dunkelkeimung bei Schizaeaceen durch Gibberellinsäure. Biol. Zentralbl. 81, 731–740

  27. Schraudolf, H. (1962) Wirkung von Hemmstoffen der DNS-, RNS-und Proteinsynthese auf Wachstum und Antheridienbildung in Prothallien von Anemia phyllitidis L. Planta 74, 123–147

  28. Shuttleworth, J., Morsek, J., Burke, D. (1982) Proteinsynthesis in human lymphoblastoid cells (Namalwa) after treatment with butyrate and 5-bromoeoxyuridine. Biochim. Biophys. Acta 698, 1–10

  29. Sina, J.F., Chin, B. (1979) Cadmium modification of nucleolar function and structure in Physarum polycephalum. Toxicol. Appl. Pharmacol. 50, 253–259

  30. Westhoff, P., Zetsche, K. (1981) Regulation of the synthesis of ribulose-1,5-biphosphate carboxylase and its subunits in flagellate Chlorogonium elongatum. Eur. J. Biochem. 116, 261–267

  31. Yamamoto, N., Sasaki, S., Asakawa, S., Hasegawa, M. (1974) Polysome formation induced by light in Pinus thunbergii seed embryos. Plant Cell Physiol. 15, 1143–1146

  32. Yamamoto, N. (1982) Preformed mRNA and light-induced germination of pine (Pinus thunbergii) seed. Plant Cell Physiol. 23, 865–869

Download references

Author information

Correspondence to H. Schraudolf.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fechner, A., Schraudolf, H. Translation and transcription in imbibed and germinating spores of Anemia phyllitidis L.Sw.. Planta 161, 451–458 (1984). https://doi.org/10.1007/BF00394577

Download citation

Key words

  • Anemia (fern)
  • Germination (mRNA)
  • Pteridophyta
  • Spore (germination)
  • Translation
  • Transcription