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In-vivo phytochrome control of in vitro transcription rates in isolated nuclei from oat seedlings


The transcription rate of isolated nuclei from oat seedlings was analysed by measuring the incorporation of radioactive label in trichloroacetic-acid-pelletable material. It was observed that this transcription rate depends on the light pretreatment of the plants: 7.5 h after a 5-min red or far-red light pulse maximum rates were found, but at this time far-red light induced only about half of the stimulation observed after a red light pulse. The far-red reversibility of the effect of the red light pulse indicates that phytochrome controls the capacity for transcription by isolated nuclei. Besides this slow reaction, phytochrome regulated the transcription rates in a very fast response when homogenates were irradiated.

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far-red light


red light



Pr, Pfr :

phytochrome in its red- or far-red-absorbing form


trichloroacetic acid


uridine 5′-monophosphate


uridine 5′-triphosphate


  1. Apel, K. (1979) Phytochrome-induced appearance of mRNA activity for the apoprotein of the light-harvesting chlorophyll a/b protein of barley. Eur. J. Biochem. 97, 183–188

  2. Blackburn, P. (1979) Ribonuclease inhibitor from human placenta: rapid purification and assay. J. Biol. Chem. 254, 12484–12487

  3. Colbert, J.T., Hershey, H.P., Quail, P.M. (1983) Autoregulatiory control of translatable phytochrome mRNA levels. Proc. Natl. Acad. Sci. USA 80, 2248–2252

  4. Fukshansky, L., Schäfer, E. (1983) Models in photomorphogenesis. In: Encyclopedia of plant physiology, N.S. vol. 16 A: Photomorphogenesis, pp. 69–95, Shrophire, W., Jr., Mohr, H., eds. Springer, Berlin Heidelberg New York

  5. Gallagher, T.F., Ellis, J.R. (1982) Light-stimulated transcription of genes for two chloroplast polypeptides in isolated pea leaf nuclei. EMBO J. 1, 1493–1498

  6. Gollmer, J., Apel, K. (1983) The phytochrome-controlled accumulation of mRNA sequences encoding the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare L.). Eur. J. Biochem. 133, 309–313

  7. Gottmann, K., Schäfer, E. (1982) In vitro synthesis of phytochrome apoprotein directed by mRNA from light- and dark-grown Avena seedlings. Photochem. Photobiol. 35, 521–525

  8. Heim, B., Schäfer, E. (1984) The effect of red and far-red light in the high irradiance reaction of phytochrome (hypocotyl growth in the dark-grown Sinapis alba L.) Plant Cell Environ. 7, 39–44

  9. Hendricks, S.B., Van Der Woude, W.J. (1983) How phytochrome acts — perspectives on the continuing quest. In: Encyclopedia of plant physiology, N.S., vol. 16 A: Photomorphogenesis, pp. 3–23, Shropshire, W., Jr., Mohr, M., eds. Springer, Berlin Heidelberg New York

  10. Hock, B., Mohr, H. (1964) Die Regulation der O2-Aufnahme von Senfkeimlingen (Sinapis alba L.) durch Licht. Planta 61, 209–228

  11. Jensen, W.A. (1962) Botanical histochemistry, pp. 250–251. Freeman, San Francisco London

  12. Luthe, D.S., Quatrano, R.S. (1980) Transcription in isolated wheat nuclei. Plant Physiol. 65, 309–313

  13. Marzluff, W.F., Jr. (1978) Transcription of RNA in isolated nuclei. Methods Cell Biol. 21, 317–332

  14. Mohr, H. (1972) Lectures on photomorphogenesis. Springer, Berlin Heidelberg New York

  15. Oelze-Karow, H., Mohr, H. (1982) Phytochrome action on chlorophyll synthesis — a study of the escape from photoreversibility. Plant Physiol. 70, 863–866

  16. Otto, V., Mösinger, E., Sauter, M., Schäfer, E. (1983) Phytochrome-control of its own synthesis in Sorghum vulgare and Avena sativa. Photochem. Photobiol. 38, 693–700

  17. Schäfer, E. (1982) Advances in photomorphogenesis. Photochem. Photobiol. 35, 905–910

  18. Sen Gupta, D.N., Sen, S.P. (1982) Phytochrome regulation of RNA synthesis in isolated coconut nuclei. Plant Cell Physiol. 23, 1251–1258

  19. Sil, P., Biswas, B.B. (1979) Action of light on in vitro transcription by plant cell nuclei. In: Genome organisation and expression in plants. Book of Abstracts, Nato Advanced Studies Institute and FEBS Advanced Course, Edinburgh

  20. Silverthorne, J., Tobin, E.M. (1984) Demonstration of transcriptional regulation of specific genes by phytochrome action. Proc. Natl. Acad. Sci. USA 81, 1112–1116

  21. Stiekema, W.J., Wimpee, L.F., Silverthorne, J., Tobin, E.M. (1983) Phytochrome control of the expression of two nuclear genes encoding chloroplast proteins in Lemna gibba. Plant Physiol. 72, 717–724

  22. Tobin, E.M., Silverthorne, J., Stiekema, W.J., Wimpee, C.F. (1983) Symposium on chloroplast biogenesis, Leiden, Ellis, R.J., ed. Cambridge University Press, Cambridge, UK

  23. Verbelen, J.-P., Pratt, L.H., Butler, W.L., Tokuyasu, K. (1982) Localization of phytochrome in oats by electron microscopy. Plant Physiol. 70, 867–871

  24. Willmitzer, L., Wagner, K.G. (1981) The isolation of nuclei from tissue-cultured plant cells. Exp. Cell. Res. 135, 69–77

  25. Wollghien, R. (1982) RNA polymerase and regulation of transcription. In: Encyclopedia of plant physiology, N.S., vol. 14 B: Nucleic acids and proteins in plants II, pp. 125–170, Dirsen, A., Zimmermann, M.H., eds. Springer, Berlin Heidelberg New York

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Mösinger, E., Schäfer, E. In-vivo phytochrome control of in vitro transcription rates in isolated nuclei from oat seedlings. Planta 161, 444–450 (1984).

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Key words

  • Avena (phytochrome)
  • Nucleus (transcription)
  • Photoinduction (phytochrome)
  • Phytochrome (transcription)
  • Transcription (phytochrome)