Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Utilization of cyclohexanone and related substances by a Nocardia sp.

  • 61 Accesses

  • 9 Citations

Abstract

A species of the genus Nocardia that could utilize cyclohexanone as a sole carbon source was isolated from soil. Cyclohexanone-grown cultures grew readily on cyclohexanol, cis, trans-cyclohexane-1,2-diol, cis-cyclohexane-1,2-diol, adipic acid and 2-hydroxycyclohexane-1-one without a noticeable lag period. The bacterium also grew on pimelic acid but only after a lag period of 4 days. Resting cell suspensions of cyclohexanone-grown cells were found to oxidize cyclohexanone, cyclohexanol, cyclohexane-1,2-dione, cis, trans-cyclohexane-1,2-diol and 2-hydroxycyclohexane-1-one at high \({\text{Q}}_{{\text{O}}_{\text{2}} }\) values. Evidence was obtained that indicated that the bacterium degraded cyclohexanone via 2-hydroxycyclohexane-1-one.

This is a preview of subscription content, log in to check access.

References

  1. Colla, C. e Treccani, V. 1960. Metabolismo ossidativo microbico degli idrocarburi ciclici saturi. Nota I: Risonossimento dell' acido adipico come intermedio dell' ossidazione della decalina. — Annali Microbiol. 10: 77–81.

  2. Feigl, F. 1966. Spot tests in organic analysis. 7th ed. — Elsevier Publishing Co., Amsterdam.

  3. Imelik, B. 1948. Oxydation du cyclohexane par Pseudomonas aeruginosa. — C. R. Acad. Sci., Paris. 226: 2082–2083.

  4. Lijmbach, G. W. M. and Brinkhuis, E. 1973. Microbial degradation of secondary n-alkyl sulfates and secondary alkanols. — Antonie van Leeuwenhoek 39: 415–423.

  5. Newton, K. G. 1971. Studies on a group of actinomycetes.—PhD thesis University of Queensland, Australia.

  6. Norris, D. B. and Trudgill, P. W. 1971. The metabolism of cyclohexanol by Nocardia globerula CL1. — Biochem. J. 121: 363–370.

  7. Ooyama, J. and Foster, J. W. 1965. Bacterial oxidation of cyclo-paraffinic hydrocarbons. —Antonie van Leeuwenhoek 31: 45–65.

  8. Posternak, T., Reymond, D. et Friedli, M. 1955. Recherches dans la série des cyclitals. XXII. Configuration et oxydation biochimique de cyclane-diols-1,2. — Helv. chim. Acta. 38: 205–212.

  9. Skerman, V. B. D. and MacRae, I. C. 1957. The influence of oxygen availability on the degree of nitrate reduction by Pseudomonas denitrificans. — Can. J. Microbiol. 3: 505–530.

  10. Umbreit, W. W., Burris, R. H. and Stauffer, J. F. 1964. Manometric techniques. — Burgess, Minneapolis.

  11. Yugari, Y. 1961. Metabolism of cyclohexane-diol-(1,2)-trans by a soil bacterium. — Biken's J. 4: 197–207.

Download references

Author information

Additional information

This work was supported by a grant from the Australian Research Grants Committee.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Murray, J.R., Scheikowski, T.A. & MacRae, I.C. Utilization of cyclohexanone and related substances by a Nocardia sp.. Antonie van Leeuwenhoek 40, 17–24 (1974). https://doi.org/10.1007/BF00394549

Download citation

Keywords

  • Carbon Source
  • Cell Suspension
  • Diol
  • Dione
  • Sole Carbon Source