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Planta

, Volume 157, Issue 1, pp 74–80 | Cite as

In vivo and in vitro synthesis of CM-proteins (A-hordeins) from barley (Hordeum vulgare L.)

  • Javier Paz-Ares
  • Fernando Ponz
  • Cipriano Aragoncillo
  • Carlos Hernández-Lucas
  • Gabriel Salcedo
  • Pilar Carbonero
  • Francisco García-Olmedo
Article

Abstract

CM-proteins from barley endosperm (CMa, CMb, CMc, CMd), which are the main components of the A-hordein fraction, are synthesized most actively 10 to 30 d after anthesis (maximum at 15–20 d). They are synthesized by membranebound polysomes as precursors of higher apparent molecular weight (13,000–21,000) than the mature proteins (12,000–16,000). The largest in vitro product (21,000) is the putative precursor of protein CMd (16,000), as it is selected with anti-CMd monospecific IgG's, and is coded by an mRNA of greater sedimentation coefficient (9 S) than those encoding the other three proteins (7.5 S). CM-proteins always appear in the soluble fraction, following different homogenization and subcellular fractionation procedures, indicating that these proteins are transferred to the soluble fraction after processing.

Key words

A-hordein CM-proteins Endosperm Hordeum (A-hordeins) Protein biosynthesis 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Javier Paz-Ares
    • 1
  • Fernando Ponz
    • 1
  • Cipriano Aragoncillo
    • 1
  • Carlos Hernández-Lucas
    • 1
  • Gabriel Salcedo
    • 1
  • Pilar Carbonero
    • 1
  • Francisco García-Olmedo
    • 1
  1. 1.Departamento de BioquímicaE.T.S. Ingenieros Agrónomos-Universidad PolitecnicaMadrid-3Spain

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