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An endogenous RNA-synthesis-promoting oligopeptide from Cucurbita pepto var. patissonina

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The effect of CPPTI (Cucurbita pepo patissonina trypsin inhibitor) on the growth of Cucurbita pepo var patissonina (White bush) was examined. Plants treated with CPPTI grew faster than untreated plants and similarly to those treated with gibberellic acid. Isolated cell nuclei from plants treated with CPPTI showed that of the three DNA-dependent RNA polymerases assayed, RNA polymerase I (EC2.7.7.6) activity was preferentially elevated.

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Cucurbita pepo patissonina trypsin inhibitor

GA3 :

gibberellic acid


indole-3-acetic acid




DNA-dependent RNA polymerase I


  1. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–689

  2. Leluk, J., Otlewski, J., Wieczorek, M., Polanowski, A., Wilusz, T. (1983) Preparation and characteristics of trypsin inhibitors from the seeds of squash and zucchini. Acta Biochim. Polon. 30, 127–138

  3. Liu, S.H., Rose, K.M. (1986) Lipopolysaccharide-mediated induction of RNA polymerase I activity and amount in murine B lymphocytes. J. Biol. Chem. 261, 8520–8527

  4. Luthe, D.S., Quatrano, R.S. (1980) Transcription in isolated wheat nuclei. I. Isolated of nuclei and elimination of endogenous ribonuclease activity. Plant Physiol. 65, 305–308

  5. Rose, K.M., Stetler, D.A., Jacob, S.T. (1981) Protein kinase activity of RNA polymerase I purified from a rat hepatoma: probable function of Mr 42000 and 24600 polypeptides. Proc. Natl. Acad. Sci. USA 78, 2833–2837

  6. Szopa, J., Rose, K.M. (1986) Cleavage of the 190-kDa subunit of DNA-dependent RNA polymerase I yields small polypeptides capable of degrading DNA. J. Biol. Chem. 261, 9022–9028

  7. Szopa, J., Rzepecki, R., Wojtkiewicz, B., Szmidziński, R. (1985) An endonuclease closely associated with DNA-dependent RNA polymerase II. FEBS Lett. 189, 301–304

  8. Szopa, J., Wagner, K.G. (1984) DNA-dependent RNA polymerase II from nuclei of suspension-cultured tobacco cells. Planta 162, 132–138

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Correspondence to Jan Szopa.

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Wiśniowska, J., Rudnicki, K., Leluk, J. et al. An endogenous RNA-synthesis-promoting oligopeptide from Cucurbita pepto var. patissonina . Planta 173, 46–51 (1988). https://doi.org/10.1007/BF00394486

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Key words

  • Cucurbita
  • Phytohormone
  • RNA polymerase