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A glucan from the cell wall of the cyanobacterium Spirulina platensis

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Abstract

A polysaccharide was isolated from the cell wall of the cyanobacterium Spirulina platensis. Hydrolysis of the polysaccharide only yielded glucose. Curie-point pyrolysis mass spectrometry of the polysaccharide resulted in a spectrum very similar to that of β-1,2-glucan. Probably the glucan originates from the fibrillar inner layer of the cell wall.

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References

  1. Drews, G. 1973. Fine structure and chemical composition of the cell envelopes, p. 99–116. In W. G. Carr and B. A. Whitton, (eds), The biology of blue-green algae. — Blackwell, Oxford.

  2. Drews, G. und Gollwitzer, W. 1965. Untersuchungen an der Polysaccharidfraktion der Zellwände von Anacystic nidulans. — Arch. Mikrobiol. 51: 179–185.

  3. Dunn, J. H. and Wolk, C. P. 1970. Composition of the cellular envelopes of Anabaena cylindrica. — J. Bacteriol. 103: 153–158.

  4. Golecki, J. R. 1977. Studies on ultrastructure and composition of cell walls of the cyanobacterium Anacystis nidulans. — Arch. Microbiol. 114: 35–41.

  5. Hisamatsu, M., Amemura, A., Harada, T., Nakanishi, I. and Kimura, K. 1977. Change in ability Agrobacterium to produce water-soluble and water-insoluble β-glucans. — J. Gen. Microbiol. 130: 374–379.

  6. Höcht, H., Martin, H.H. und Kandler, O. 1965. Zur Kenntnis der chemischen Zusammensetzung der Zellwand der Blaualgen. — Z. Pflanzenphysiol. 53: 39–57.

  7. Meuzelaar, H. L. C., Kistemaker, P. G. and Posthumus, M. A. 1974. Recent advances in pyrolysis mass spectrometry of complex biological materials. — Biomed. Mass Spectrom. 1: 312–319.

  8. Nakanishi, I., Kimura, K., Suzuki, T., Ishikawa, M., Banno, I., Sakane, T. and Harada, T. 1976. Demonstration of curdlan-type polysaccharide and some other β-1,3-glucans in microorganisms with aniline blue. — J. Gen. Appl. Microbiol. 22: 1–11.

  9. Ogawa, T. and Terui, G. 1970. Studies on the growth of Spirulina platensis. — J. Ferment. Technol. 48: 361–367.

  10. Patridge, S. M. 1949. Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars. — Nature 164: 443.

  11. Posthumus, M. A., Boerboom, A. J. H. and Meuzelaar, H. L. C. 1974. Analysis of biopolymers by Curie-point pyrolysis in direct combination with low voltage electron impact ionization mass spectrometry, p. 397–402. In A. R. West (ed.), Advances in mass spectrometry, Vol. 6. — Applied Science, Barking, Essex.

  12. Schaefer, H. P. 1977. An alkali-soluble polysaccharide from the cell walls of Coprinus lagopus. —Arch. Microbiol. 113: 79–82.

  13. Schulten, H. R., Beckley, H. O., Meuzelaar, H. L. C. and Boerboom, A. J. H. 1973. High resolution field ionization mass spectrometry of bacterial pyrolysis products. — Anal. Chem. 45: 191–195.

  14. Schulten, H. R. 1975. High resolution field ionization and field desorption mass spectrometry of pyrolysis products of complex organic materials, p. 155–164. In C. G. Hedén and T. Illénie, (eds), New approaches to the identification of microorganisms. — John Wiley and Sons, New York.

  15. Shafizadeh, F. 1968. Pyrolysis and combustion of cellulosic material. — Adv. Carbohydrate Chem. 23: 419–474.

  16. Soeder, C. J. 1976. Zur Verwendung von Mikroalgen für Ernährungszwecker. — Naturwissenschaften 63: 131–138.

  17. Trevelyan, W. E., Proctor, D. P. and Harrison, J. S. 1950. Detection of sugars on paper chromatograms. — Nature 166: 444–445.

  18. Van Eykelenburg, C. 1977. On the morphology and ultrastructure of the cell wall of Spirulina platensis. — Antonie van Leeuwenhoek 43: 89–99.

  19. Weyman, A. C. M. 1976. Cell wall composition and taxonomy of Cephaloascus fragrans and some Ophiostomataceae. — Antonie van Leeuwenhoek 42: 315–324.

  20. Weyman, A. C. M. 1977. The application of Curie-point pyrolysis mass spectrometry in fungal taxonomy, p. 225–234. In C. E. Roland Jones and C. A. Cramers (eds.), Analytical pyrolysis. —Elsevier, Amsterdam.

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van Eykelenburg, C. A glucan from the cell wall of the cyanobacterium Spirulina platensis . Antonie van Leeuwenhoek 44, 321–327 (1978). https://doi.org/10.1007/BF00394309

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Keywords

  • Glucose
  • Mass Spectrometry
  • Cell Wall
  • Polysaccharide
  • Glucan