Marine Biology

, Volume 41, Issue 2, pp 153–177 | Cite as

Effect of salinity fluctuation on the osmotic pressure and Na+, Ca2+ and Mg2+ ion concentrations in the hemolymph of bivalve molluscs

  • S. E. Shumway


Specimens of Chlamys opercularis, Modiolus modiolus, Mytilus edulis, Crassostrea gigas, Scrobicularia plana and Mya arenaria were exposed to both gradual (sinusoidal) and abrupt (square-wave) salinity fluctuations and measurements made of osmotic, Na+, Mg2+ and Ca2+ concentrations in the hemolymph and where applicable in the mantle fluid. In both sinusoidal and square-wave regimes fluctuating between 100 and 50% seawater (100%=ca. 32‰ S), the hemolymph Na+, Mg2+, Ca2+ and osmotic concentrations followed the concentrations of the external medium in Chlamys opercularis. The hemolymph and mantle fluid osmotic Na+, Mg2+ and Ca2+ concentrations of Modiolus modiolus, Mytilus edulis, Crassostrea gigas and S. plana followed those of the external medium as long as the molluscs' shell valves remained open. There were no changes in the ionic or osmotic concentrations of the hemolymph or mantle fluid of any of these species during periods of shell-valve closure. The hemolymph osmotic, Na+ and Mg2+ concentrations of wedged-open Modiolus modiolus, Mytilus edulis, C. gigas and S. plana followed those of the external medium. Hemolymph Ca2+ concentrations showed a damped response in C. gigas and Mytilus edulis. The hemolymph osmotic, Na+, Ca2+ and Mg2+ concentrations of Mya arenaria fluctuated in a similar manner to the external medium, but were damped. Wedged-open Mytilus edulis exposed to fluctuating salinity and supplied with a constant supply of 10 mM Ca2+ showed greater changes in hemolymph ionic and osmotic concentrations than M. edulis exposed to the same salinity fluctuation without a constant Ca2+ supply. Chlamys opercularis and Modiolus modiolus survived in a 50% seawater minimum sinusoidal salinity fluctuation for 10 days; wedged-open M. modiolus survived only 3 days. Burrowing had no effect on the osmotic, Na+, Mg2+ or Ca2+ concentrations of the hemolymph of Mya arenaria or S. plana exposed to fluctuating salinities. All of the species studied were shown to be osmoconformers.


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Literature Cited

  1. Anderson, J.D. and C.L. Prosser: Osmoregulating capacity in populations occurring in different salinities. Biol. Bull. mar. biol. Lab., Woods Hole 105, p. 369 (1953)Google Scholar
  2. Basindale, R.: A comparison of the varying salinity conditions of the Tees and Severn estuaries. J. Anim. Ecol. 12, 1–10 (1943)Google Scholar
  3. Bedford, W.B. and J.W. Anderson: The physiological response of the estuarine clam, Rangia cuneata (Gray) to salinity. I Osmoregulation. Physiol. Zoöl. 45, 255–260 (1972)Google Scholar
  4. Brand, A.R. and D. Roberts: The cardiac responses of the scallop Pecten maximus to respiratory stress. J. exp. mar. Biol. Ecol. 13, 29–43 (1973)Google Scholar
  5. Chapman, G. and G.E. Newell: The role of the body fluid in the movement of soft-bodied invertebrates. II. The extension of the siphons of Mya arenaria and Scrobicularia plana. Proc. R. Soc. (Ser. B) 145, 564–580 (1956)Google Scholar
  6. Coleman, N. and E.R. Trueman: The effect of aerial exposure on the activity of the mussels Mytilus edulis and Modiolus modiolus. J. exp. mar. Biol. Ecol. 7, 295–304 (1971)Google Scholar
  7. Conway, E.J.: Fundamental problems in hormonal control of water and salt electrolyte metabolism. In: The hormonal control of water and salt-electrolyte metabolism in vertebrates, Vol. 5. Pt. II. pp 3–22. Ed. by I.C. Jones and P. Eckstein. London: Cambridge University Press 1956 (Mem. Soc. exp. Endocr.)Google Scholar
  8. —: Principles underlying the exchange of sodium and potassium ions across cell membranes. J. gen. Physiol. 43, (Suppl. 1), 17–41 (1960)Google Scholar
  9. Davenport, J., Ll.D. Gruffydd and A.R. Beaumont: An apparatus to supply water of fluctuating salinity and its use in a study of the salinity tolerance of larvae of the scallop Pecten maximus L. J. mar. biol. Ass. U.K. 55, 391–409 (1975)Google Scholar
  10. Fingerman, M. and L.D. Fairbanks: Osmotic behaviour and bleeding of the oyster, Crassostrea virginica. Tulane Stud. Zool. 3, 151–168 (1956)Google Scholar
  11. Freeman, R.F.H. and F.H. Rigler: The responses of Scrobicularia plana (da Costa) to osmotic pressure changes. J. mar. biol. Ass. U.K. 36, 553–567 (1957)Google Scholar
  12. Galstoff, P.S.: The American oyster. Fishery Bull. Fish Wildl. Serv. U.S. 64, 1–480 (1964)Google Scholar
  13. Ghiretti, F.: Respiration. In: Physiology of Mollusca, pp 175–208. Ed. by K.M. Wilbur and C.M. Yonge, New York: Academic Press 1966Google Scholar
  14. Gilles, R.: Osmoregulation in three molluscs: Acanthochitona discrepans (Brown), Glycymeris glycymeris and Mytilus edulis. Biol. Bull. mar. biol. Lab., Woods Hole 142, 25–35 (1972)Google Scholar
  15. Gray, J.: The mechanisms of ciliary movement. IV. The relation of ciliary activity to oxygen consumption. Proc. R. Soc. (Ser. B) 96, 95–114 (1924)Google Scholar
  16. Green, J.: The biology of estuarine animals, 401 pp. London: University of Washington 1968Google Scholar
  17. Hayes, F.R. and D. Pelluet: The inorganic constitution of molluscan blood and muscle. J. mar. biol. Ass. U.K. 26, 580–589 (1947)Google Scholar
  18. Hopkins, A.E.: Adaptation of the feeding mechanism of the oyster (Ostrea gigas) to changes in salinity. Bull. Bur. Fish., Wash. 48, 345–363 (1936)Google Scholar
  19. Hoyeaux, J., R. Gilles and C. Jeuniaux: Osmoregulation in molluscs of the intertidal zone. Comp. Biochem. Physiol. 53A, 361–365 (1976)Google Scholar
  20. Kinne, O.: Salinity: animals — invertebrates. In: Marine ecology. Vol. I. Environmental factors, Part 2. pp 821–995. Ed. by O. Kinne. London: Wiley Interscience 1971Google Scholar
  21. Loosanoff, V.L.: Behavior of oysters in water of low salinities. Conv. Addr. natn. Shellfish. Ass. 1, 135–151 (1952)Google Scholar
  22. — and P.B. Smith: Some aspects of behavior of oysters accustomed to different salinities. Anat. Rec. 105, p. 627 (1949)Google Scholar
  23. Matthiessen, G.C.: Observations on the ecology of the soft clam Mya arenaria in a salt pond. Limnol. Oceanogr. 5, 291–300 (1960)Google Scholar
  24. Milne, A.: The ecology of the Tamar estuary III. Salinity and temperature conditions in the lower estuary. J. mar. biol. Ass. U.K. 22, 529–542 (1938)Google Scholar
  25. Pantin, C.F.A.: On the physiology of amoeboid movement. III. The action of calcium. Br. J. exp. Biol. 3, 275–295 (1926)Google Scholar
  26. Papero, A. and J.A. Murphy: The effect of calcium on the rate of beating of lateral cilia in Mytilus edulis. I. A response to perfusion with 5-HT, DA, BOL and PBZ. Comp. Biochem. Physiol. 50C, 15–20 (1975)Google Scholar
  27. Perkin-Elmer: Analytical methods for atomic adsorption spectrophotometry, p. EN-1. Norwich, Connecticut: Perkin Elmer 1973Google Scholar
  28. Pierce, S.K.: The water balance of Modiolus (Mollusca: Bivalvia: Mytilidae): osmotic concentrations in changing salinities. Comp. Biochem. Physiol. 36, 521–533 (1970)Google Scholar
  29. Potts, W.T.W. and G. Parry: Osmotic and ionic regulations in animals, 433 pp. London: Pergamon Press 1964Google Scholar
  30. Prosser, C.L.: Comparative animal physiology, 456 pp. Philadelphia: W.B. Saunder's Co. 1973Google Scholar
  31. Ricci, E.: L'adaptation de la moule commune. Mytilus galloprovincialis aux changements de salinité. Annls Fac. Sci. Marseille 12, 47–91 (1939)Google Scholar
  32. Robertson, J.D.: The function and metabolism of calcium in the Invertebrata. Biol. Rev. 16, 106–133 (1941)Google Scholar
  33. —: Ionic regulation in some marine invertebrates. J. exp. Biol. 26, 182–200 (1949)Google Scholar
  34. —: Osmotic and ionic regulation. In: Physiology of the Mollusca, Vol. I. pp 283–311. Ed. by K.M. Wilbur and C.M. Yonge. New York: Academic Press 1964Google Scholar
  35. Schlieper, C.: Osmotic resistance of gills of Mytilus. Helgoländer wiss. Meeresunters. 14, 482–502 (1966)Google Scholar
  36. Schoffeniels, E. and R. Gilles: Ionoregulation and osmoregulation in mollusks. In: Chemical zoology, Vol. 7. pp 393–420. Ed. by M. Florkiss and B. Scheer. New York: Academic Press 1970Google Scholar
  37. Stickle, W.B. and R. Ahokas: The effects of tidal fluctuation of salinity on the perivisceral fluid composition of several echinoderms. Comp. Biochem. Physiol. 47A, 469–476 (1973)Google Scholar
  38. ——: The effects of salinity on the hemolymph composition of several molluscs. Comp. Biochem. Physiol. 50A, 291–296 (1975)Google Scholar
  39. Stickney, R.R.: Determination of sodium, calcium, magnesium and potassium in channel catfish blood plasma. Bull. Ga Acad. Sci. 29, 163–168 (1971)Google Scholar
  40. Topping, F.L. and J.L. Fuller: The accommodation of some marine invertebrates to reduced osmotic pressure. Biol. Bull. mar. biol. Lab., Woods Hole 82, 372–384 (1942)Google Scholar
  41. Tucker, L.E.: Effects of external salinity on Scutus breviculus (Gastropoda, Prosobranchia) — I. Body weight and blood composition. Comp. Biochem. Physiol. 36, 301–319 (1970)Google Scholar
  42. Van Dam, L.: On the utilization of oxygen by Mya arenaria. J. exp. Biol. 12, 86–97 (1935)Google Scholar
  43. Webb, D.A.: Ionic regulation in Carcinus maenas. Proc. R. Soc. (Ser. B) 129, 107–136 (1940)Google Scholar
  44. Webb, J.L.: The ecology of Lagos lagoon, III. The life history of Branchiostoma nigeriense Webb. Phil. Trans. R. Soc. (Ser. B) 241, 335–353 (1958)Google Scholar
  45. Yazaki, M.: On the osmoregulation of the blood of several marine and fresh water molluscs. I. The Japanese oyster, Ostrea circumpicta. Sci. Rep. Tôhoku Univ. (Ser. 4) 7, 229–238 (1932)Google Scholar
  46. Zachary, A. and D.S. Haven: Survival and activity of the oyster drill Urosalpinx cinerea under conditions of fluctuating salinity. Mar. Biol. 22, 45–52 (1973)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • S. E. Shumway
    • 1
  1. 1.Marine Science LaboratoriesN.E.R.C. Unit of Marine Invertebrate BiologyMenai BridgeUK

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