Antonie van Leeuwenhoek

, Volume 46, Issue 6, pp 601–610 | Cite as

Acetate, methanol and carbon dioxide as substrates for growth of Methanosarcina barkeri

  • T. J. Hutten
  • H. C. M. Bongaerts
  • C. van der Drift
  • G. D. Vogels
Physiology and Growth


Methanosarcina barkeri grows in defined media with acetate, methanol or carbon dioxide as carbon sources. Methanol is used for methanogenesis at a 5 times higher rate as compared with the other substrates. M. barkeri can use the substrates simultaneously, but due to acidification or alkalification of the medium during growth on methanol or acetate, respectively, growth and methanogenesis may stop before the substrates are exhausted. Growth and methanogenesis on methanol or acetate are inhibited by the presence of an excess of H2; the inhibition is abolished by the addition of carbon dioxide, which probably serves as an essential source of cell carbon, in the absence of which methano-genesis ceases.


Acetate Methanol Dioxide Carbon Dioxide Carbon Source 
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Copyright information

© H. Veenman en Zonen 1980

Authors and Affiliations

  • T. J. Hutten
    • 1
  • H. C. M. Bongaerts
    • 1
  • C. van der Drift
    • 1
  • G. D. Vogels
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceUniversity of NijmegenNijmegenThe Netherlands

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