Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

The influence of cell ploidy on the thermotolerance of Saccharomyces cerevisiae

  • 28 Accesses

  • 9 Citations

Summary

The resistance of Saccharomyces cerevisiae to inactivation by DNA damaging agents has long been known to be affected by cell ploidy. Resistance is greater for diploid than for haploid cells, but exhibits decreases for further increases in ploidy beyond diploid. In this study S. cerevisiae cells whose genomes differ only in their ploidy were employed to investigate how ploidy directly influences resistance to thermal killing. In virtually all species resistance to thermal killing is a cellular property that is elevated by heat shock and other agents that induce the heat shock response. We therefore investigated how ploidy affected the thermal killing of S. cerevisiae cells both before and after elevation of thermotolerance by means of a 40 min 25 °C to 38 °C heat shock. Without such induction of thermotolerance there was negligible effect of ploidy on thermal killing. In contrast in the heat shocked cultures there was an appreciable decrease in thermotolerance as ploidy increased. This difference indicates that the lethal thermal damage in the thermotolerance induced cultures is not totally equivalent to that in cells not given a prior heat shock, and that gene expression changes after heat shock result in a ploidy effect on heat tolerance which is absent from cells in which the heat shock response has not been induced.

This is a preview of subscription content, log in to check access.

References

  1. Craig EA, Jacobsen K (1985) Mol Cell Biol 5:3517–3524

  2. Haynes RH, Kunz BA, Strathern JN, Jones EW, Broach JR (eds) (1983) In: The molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, New York, pp 371–414

  3. Iida H, Yahara I (1985) Nature 315:688–690

  4. Madiera-Lopes A (1982) Cienc Biol (Portugal) 7:59–64

  5. McAlister L, Finkelstein DB (1980) Biochem Biophys Res Commun 93:819–824

  6. McAlister L, Strausberg S, Kulaga A, Finkelstein D (1979) Curr Genet 1:63–74

  7. McClanahan T, McEntee K (1986) Mol Cell Biol 6:90–96

  8. Miller MJ, Xuong NH, Geiduschek EP (1979) Proc Natl Acad Sci USA 76:5222–5225

  9. Mitchel REJ, Morrison DP (1982) Radiat Res 92:192–199

  10. Mitchel REJ, Morrison DP (1986) Mutat Res 159:31–39

  11. Mizusawa S, Gottesman S (1983) Proc Natl Acad Sci USA 80:358–362

  12. Mortimer RK (1958) Radiat Res 9:312–326

  13. Neidhardt FC, VanBogelan RA, Vaughn V (1984) Ann Rev Genet 18:295–329

  14. Owen ME, Mortimer RK (1956) Nature 177:625–626

  15. Pelham H (1985) Trends Genet 1:31–35

  16. Pelham H (1986) Cell 46:959–961

  17. Piper PW, Curran B, Davies W, Lockheart A, Reid G (1986) Eur J Biochem 161:525–531

  18. Plesset J, Palm C, McLaughlin CS (1982) Biochem Biophys Res Commun 108:1340–1345

  19. Sarachek A (1954) Cytologia 19:77–87

  20. Sudbery PE, Goodey AR, Carter BLA (1980) Nature 288:401–404

  21. Takagi A, Harashima S, Oshima Y (1985) Appl Environ Microbiol 49:244–246

  22. Van Uden N, Madiera-Lopes A (1975) Arch Microbiol 104:23–28

  23. Walker GC (1984) Microbiol Rev 48:60–93

Download references

Author information

Correspondence to Peter W. Piper.

Additional information

Communicated by B. S. Cox

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Piper, P.W., Davies, M.W., Curran, B. et al. The influence of cell ploidy on the thermotolerance of Saccharomyces cerevisiae . Curr Genet 11, 595–598 (1987). https://doi.org/10.1007/BF00393921

Download citation

Key words

  • Heat shock
  • Thermotolerance
  • Ploidy
  • Yeast