, Volume 156, Issue 3, pp 270–273 | Cite as

Composition of the cell wall of Chlorella fusca

  • Eckhard Loos
  • Doris Meindl


Isolated cell walls of mature Chlorella fusca consisted of about 80% carbohydrate, 7% protein, and 13% unidentified material. Mannose and glucose were present in a ratio of about 2.7:1 and accounted for most of the carbohydrate. Minor components were glucuronic acid, rhamnose, and traces of other sugars; galactose was absent. After treatment with 2 M trifluoroacetic acid or with 80% acetic acid/HNO3 (10/1, v/v), a residue with a mannose/glucose ratio of 0.3:1 was obtained, probably representing a structural polysaccharide. An X-ray diffraction diagram of the walls showed one diffuse reflection at 0.44 nm and no reflections characteristic of cellulose. Walls from young cells contained about 51% carbohydrate, 12% protein, and 37% unidentified material. Mannose and glucose were also the main sugars; their absolute amounts per wall increased 6–7 fold during cell growth. Walls isolated with omission of a dodecylsulphate/mercaptoethanol/urea extraction step had a higher protein content and, with young walls, a significantly higher glucose and fucose content. These data and other published cell wall analyses show a wide variability in cell wall composition of the members of the genus Chlorella.

Key words

Cell wall (composition) Chlorella 



gas liquid chromatography


trifluoroacetic acid


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  1. Becker, M.J., Shefner, A.M. (1964) Thin-layer and paper chromatographic analyses of the carbohydrates in the cell wall of Chlorella pyrenoidosa 7-11-05. Nature (London) 202, 803–804Google Scholar
  2. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F. (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356Google Scholar
  3. Frei, E., Preston, R.D. (1961) Variants in the structural polysaccharides of algal cell walls. Nature (London) 192, 939–943Google Scholar
  4. Galambos, J.T. (1967) The reaction of carbazole with carbohydrates I. Effect of borate and sulfamate on the carbazole color of sugars. Anal. Biochem. 19, 119–132Google Scholar
  5. Ghebregzabher, M., Rufini, S., Monaldi, B., Lato, M. (1976) Thin-layer chromatography of carbohydrates. J. Chromatogr. 127, 133–162Google Scholar
  6. Iriki, Y., Miwa, T. (1960) Chemical nature of the cell wall of the green algae, Codium, Acetabularia and Halicoryne. Nature (London) 185, 178–179Google Scholar
  7. Kreger, D.R. (1962) Cell walls. In: Physiology and biochemistry of algae, pp. 315–335. Lewin, R.A., ed.. Academic Press, New York LondonGoogle Scholar
  8. Kuhl, A. (1962) Zur Physiologie der Speicherung kondensierter anorganischer Phosphate in Chlorella. In: Beiträge zur Physiologie und Morphologie der Algen, pp. 157–166, Deutsche Botanische Gesellschaft, ed. Fischer, StuttgartGoogle Scholar
  9. Lamport, D.T.A., Miller, D.H. (1971) Hydroxy-proline arabinosides in the plant kingdom. Plant Physiol. 48, 454–456Google Scholar
  10. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275Google Scholar
  11. Mankarios, A.T., Jones, C.F.G., Jarvis, M.C. (1979) Hydrolysis of plant polysaccharides and GLC analysis of their constitutent neutral sugars. Phytochemistry 18, 419–422Google Scholar
  12. Northcote, D.H., Goulding, K.J., Horne, R.W. (1958) The chemical composition and structure of the cell wall of Chlorella pyrenoidosa. Biochem. J. 70, 391–397Google Scholar
  13. Northcote, D.H., Goulding, K.J., Horne, R.W. (1960) The chemical composition and structure of the cell wall of Hydrodictyon africanum Yaman. Biochem. J. 77, 503–508Google Scholar
  14. Parker, B.C. (1964) The structure and chemical composition of cell walls in three chlorophycean algae. Phycologia 4, 63–74Google Scholar
  15. Punnet, D., Derrenbacker, E.C. (1966) The amino acid composition of algal cell walls. J. Gen. Microbiol. 44, 105–114Google Scholar
  16. Robinson, D.G., White, R.K. (1972) The fine structure of Oocystis apiculata W. West with particular reference to the wall. Br. Physiol. J. 7, 109–118Google Scholar
  17. Saeman, J.F., Buhl, J.L., Harris, E.F. (1945) Quantitative saccharification of wood and cellulose. Ind. Eng. Chem., Anal. Ed. 17, 35–37Google Scholar
  18. Takeda, H., Hirokawa, T. (1978) Studies on the cell wall of Chlorella. I. Quantitative changes in cell wall polysaccharides during the cell cycle of Chlorella ellipsoidea. Plant Cell Physiol. 19, 591–598Google Scholar
  19. Updegraff, D.M. (1969) Semimicro determination of cellulose in biological materials. Anal. Biochem. 32, 420–424Google Scholar
  20. Wolfrom, M.L., Patin, D.L., de Lederkremer, R.M. (1965) Thin-layer chromatography on microcrystalline cellulose. J. Chromatogr. 17, 488–494Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Eckhard Loos
    • 1
  • Doris Meindl
    • 1
  1. 1.Institut für Botanik der UniversitätRegensburgGermany

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