, Volume 156, Issue 3, pp 270–273

Composition of the cell wall of Chlorella fusca

  • Eckhard Loos
  • Doris Meindl

DOI: 10.1007/BF00393735

Cite this article as:
Loos, E. & Meindl, D. Planta (1982) 156: 270. doi:10.1007/BF00393735


Isolated cell walls of mature Chlorella fusca consisted of about 80% carbohydrate, 7% protein, and 13% unidentified material. Mannose and glucose were present in a ratio of about 2.7:1 and accounted for most of the carbohydrate. Minor components were glucuronic acid, rhamnose, and traces of other sugars; galactose was absent. After treatment with 2 M trifluoroacetic acid or with 80% acetic acid/HNO3 (10/1, v/v), a residue with a mannose/glucose ratio of 0.3:1 was obtained, probably representing a structural polysaccharide. An X-ray diffraction diagram of the walls showed one diffuse reflection at 0.44 nm and no reflections characteristic of cellulose. Walls from young cells contained about 51% carbohydrate, 12% protein, and 37% unidentified material. Mannose and glucose were also the main sugars; their absolute amounts per wall increased 6–7 fold during cell growth. Walls isolated with omission of a dodecylsulphate/mercaptoethanol/urea extraction step had a higher protein content and, with young walls, a significantly higher glucose and fucose content. These data and other published cell wall analyses show a wide variability in cell wall composition of the members of the genus Chlorella.

Key words

Cell wall (composition) Chlorella 



gas liquid chromatography


trifluoroacetic acid

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Eckhard Loos
    • 1
  • Doris Meindl
    • 1
  1. 1.Institut für Botanik der UniversitätRegensburgGermany

Personalised recommendations