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Marine Biology

, Volume 64, Issue 3, pp 285–289 | Cite as

Observations of the effect of zinc on the gametes and various development phases of Arbacia lixula

  • A. Castagna
  • F. Sinatra
  • M. Scalia
  • V. Capodicasa
Article
  • 49 Downloads

Abstract

The action of various concentrations of zinc on different phases of development of Arbacia lixula L. has been observed. The following concentrations were used in the experiment: 0.01, 0.1, 1 and 10 mg l-1; these concentrations were tested on unfertilized eggs, sperms, fertilized eggs and adult individuals. It was found that after 96 h a 0.01 mg l-1 concentration reduces the mobile sperm percentage compared with the control group. At concentrations of 0.1, 1 and 10 mg l-1 no mobile sperm were found after 96 h. Compared with the control group, the action of zinc on unfertilized eggs was to increase the percentage of eggs showing signs of cytolisis after 12, 36 and 60 h. In the case of fertilized eggs it was found that with a concentration of 0.01 mg l-1 the P5 pluteus stage was attainable; the percentage of forms reaching that stage was less than in the control group. At 0.1 mg l-1 only a very low percentage of eggs could reach the P5 pluteus stage; concentrations of 1 and 10 mg l-1 are incompatible with the normal development of an egg. Adult individuals kept in aquaria with 1 mg l-1 zinc showed no signs of suffering after 20 d. Gametes are proposed as the appropriate material for carrying out bioassays.

Keywords

Zinc Normal Development Development Phase Adult Individual Mobile Sperm 

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Literature Cited

  1. Barber, R. T., J. Barret, J. A. McGowen, J. G. Quinn, L. Pomeray and L. Provasoli: The effects on marine organisms. Marine environmental quality. National Acad. of Science, Wash. D.C. 63–81, 1971Google Scholar
  2. Bernhard, N. and A. Zattera: Valutazione per alcuni polluenti delle concentrazioni tollerabili nell'ambiente marino e della ingestione limite per l'uomo. Ecologia, Acqua, Aria, Suolo, 8, 612–620 (1975)Google Scholar
  3. Bryan, G. W.: Zinc regulation in the lobster Homarus vulgaris. 1: Tissue zinc and copper concentrations. J. mar. biol. Ass. U.K. 44, 549–563 (1964)CrossRefGoogle Scholar
  4. Bryan, G. W.: The metabolism of zinc in crabs, lobster and freshwater crayfish, pp 1005–1016. Ed. by B. Alberg and F. P. Ungate. Radioecological concentration process. Oxford: Pergamon Press 1967CrossRefGoogle Scholar
  5. Goldberg, E. D.: Minor elements in sea water in chemical oceanography, pp 163–196. Ed. by J. P. Riley and G. Skirrow. New York: Academic Press 1965Google Scholar
  6. Kinoshita, S.: The mode of action of metal chelating substances on sperm motility in some marine forms as shown by glyceral extracted sperm models. J. Fac. Sci. Tokyo Univ. Sec. IV, Zool. 8, 219–228 (1958)Google Scholar
  7. Kinoshita, S.: On the identity of the motility-inducing factor of flagellum and the relaxing factor of muscle. J. Fac. Sci. Tokyo Univ. Sec. IV, Zool. 8, 427–437 (1959)Google Scholar
  8. Kobayashi, N.: Fertilised sea urchin eggs as an indicator material for marine pollution bioassay, preliminary experiments. Publ. Seto mar. biol. Lab. XVIII (6), 379–406 (1971)CrossRefGoogle Scholar
  9. Kobayashi, N., H. Nagami and K. Doi: Marine pollution bioassay by using sea urchin eggs in the inland sea of Japan (the seto-Naikai). Publ. Seto mar. biol. Lab. XIX (6), 359–381 (1972)CrossRefGoogle Scholar
  10. Kobayashi, N.: Studies on the effects of some agents on fertilised sea urchin eggs, as a part of the bases for marine pollution bioassay I. Publ. Set mar. biol. Lab. XXI (2), 109–114 (1973)CrossRefGoogle Scholar
  11. Kobayashi, N.: Marine pollution bioassay by sea urchin eggs, an attempt to enhance accuracy. Publ. Seto. mar. biol. Lab. XXI (5/6) 377–391 (1974)CrossRefGoogle Scholar
  12. Lallier, R.: Effects des ions zinc et cadmium sur le développement de l'oeuf de l'oursin Paracentrotus lividus. Arch. Biol. (Liège) 66, 75–102 (1955)Google Scholar
  13. Lallier, R.: Recherches sur la détermination embryonnaire chez les Echinodermes. L'action des oxydants et de l'acide p-cloro-mercuribenzoique sur le développement de l'oeuf de l'oursin Paracentrotus lividus. Arch. Biol. 67, 181–209 (1956a)Google Scholar
  14. Lallier, R.: Les ions des metaux lourds et le problème de la détermination embryonnaire chez les Echinodermes. J. Embryol. exp. Morphol. 4, 265–278 (1956b)Google Scholar
  15. Lallier, R.: Recherches sur l'animalization de l'oeuf d'oursin par les ions zinc. J. Embryol. exp. Morphol. 7, 540–548 (1959)PubMedGoogle Scholar
  16. Mateyko, C.M.: Developmental modifications in Arbacia punctulata by various metabolic substances. Biol. Bull., mar. biol. Lab., Woods Hole 130, 184–228 (1967)CrossRefGoogle Scholar
  17. Metz, C. B. and C. W. Birky: The action of some metal ions and some metal chelating agents on the motility and respiration of the starfish sperm. Biol. Bull., mar. biol. Lab., Woods Hole 109, 365–366 (1955)Google Scholar
  18. Okubo, K. and T. Okubo: Study on the bioassay method for evaluation of water pollution. II: Use of fertilised eggs of sea urchins and bivalves. Bulletin, Tokai Regional Fisheries Research Laboratory 32, 131–140 (1962)Google Scholar
  19. Pirrone, A. M., G. Scanzo, V. Mutalo and G. Giudice: Effect of chemical animalization and vegetalization on the synthesis of ribosomial RNA in sea urchin embryos. Wilhelm Roux Arch. Entwicklungsmech. Organismen 164, 222–225 (1970)CrossRefGoogle Scholar
  20. Ranzi, S. and P. Citterio: Sulle proteine dell'Arbacia lixula nello sviluppo embrionale e postembrionale. Rend. Ist. lombardo Sci. 90, 515–521 (1956)Google Scholar
  21. Ranzi, S.: Early determination in development under normal and experimental conditions, pp 291–318 In: The beginnings of embryonic development. Washington. Am. Assoc. Advanc. Science 1957Google Scholar
  22. Ranzi, S.: On the stability of the structures of animalized and vegetalized sea urchin embryos. Biol. Bull., mar. biol. Lab., Woods Hole 171, 436–437 (1959)Google Scholar
  23. Slater, J. P., A. S. Mildvan and L. A. Loeb: Zinc in DNA polymerase. Biochem. Biophys. Res. Comm. 44, 37–43 (1971)CrossRefGoogle Scholar
  24. Steele, J. H., A. D. McIntyre, R. Johnston, J. G. Baxter, G. Topping and H. D. Dooley: Pollution studies in the Clyde sea area. Mar. Poll. Bull. 4, 153–157 (1973)CrossRefGoogle Scholar
  25. Sugiyama, M.: Polyspermy in the sea urchin eggs induced by CuSO4. Zool. Magz. 50, 11–12 (1950)Google Scholar
  26. Waterman, A. J.: Effects of salts of heavy metals on development of the sea urchin, Arbacia punctulata. Biol. Bull., mar. biol. Lab., Woods Hole 73, 401–420 (1937)CrossRefGoogle Scholar
  27. WQC: Water quality criteria 1972. Report of the Committee on water quality criteria pp 1–594. Nat. Acad. Engineering Superint. Dac. U.S. Govern. Print. Office, Washington D.C. Stock. No. 5501-00520 (1973)Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • A. Castagna
    • 1
  • F. Sinatra
    • 1
  • M. Scalia
    • 1
  • V. Capodicasa
    • 1
  1. 1.Institute of BiologyUniversity of CataniaCataniaItaly

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