Planta

, Volume 160, Issue 4, pp 320–329

Effects of partial defoliation, changes of irradiance during growth, short-term water stress and growth at enhanced p(CO2) on the photosynthetic capacity of leaves of Phaseolus vulgaris L.

  • S. von Caemmerer
  • G. D. Farquhar
Article

Abstract

The response of CO2-assimilation rate to the intercellular partial pressure of CO2 (p(CO2)) is used to analyse the effects of various growth treatments on the photosynthetic characteristics of P. vulgaris. Partial defoliation caused an increase in CO2-assimilation rate at all intercellular p(CO2). A change in the light regime for growth from high to low light levels caused a decrease of CO2-assimilation rate at all intercellular p(CO2). Growth in a CO2-enriched atmosphere resulted in lowered assimilation assimilation rates compared with controls at comparable intercellular p(CO2). Short-term water stress initially caused only a decline in the CO2-assimilation rate at high intercellular p(CO2), but not at low intercellular p(CO2). Except under severe water stress, changes in the initial slope of the response of CO2-assimilation rate to intercellular p(CO2) were in parallel to those of the in-vitro activity of ribulose-1,5-bisphosphate (RuBP) carboxylase. From the results, we infer that partial defoliation, changes in the light regime for growth, and growth in a CO2-enriched atmosphere cause parallel changes in RuBP-carboxylase (EC 4.1.1.39) activity and the “capacity for RuBP regeneration”, whereas short-term water stress initially causes only a decline in the RuBP-regeneration capacity.

Key words

Carbon dioxide (high partial pressure) Electron transport Gas exchange Phaseolus (CO2 assimilation) Photosynthesis at high p(CO2Ribulose-1,5-bisphosphate carboxylase-oxygenase Defoliation Water stress 

Abbreviations and symbols

p(CO2)

partial pressure(s) of carbon dioxide

RuBP

ribulose-1,5-bisphosphate

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References

  1. Alderfer, R.G., Eagles, C.F. (1976) The effect of partial defoliation on the growth and photosynthetic efficiency of bean leaves. Bot. Gaz. (Chicago) 137, 351–355Google Scholar
  2. Badger, M.R., Collatz, G.J. (1977) Studies on the kinetic mechanism of ribulose-1,5-bisphosphate carboxylase and oxygenase reactions, with particular reference to the effect of temperature on kinetic parameters. Carnegie Inst. Washington Yearb. 76, 355–361Google Scholar
  3. Ball, M.C., Farquhar, G.D. (1984a) Photosynthetic and stomatal responses of two mangrove species, Aegiceras corniculatum and Avicennia marina, to long term salinity and humidity conditions. Plant Physiol. (in press)Google Scholar
  4. Ball, M.C., Farquhar, G.D. (1984b) Photosynthetic and stomatal responses of the grey mangrove, Avicennia marina, to transient salinity conditions. Plant Physiol. (in press)Google Scholar
  5. Björkman, O. (1982) Responses to different quantum flux densities. In: Encyclopedia of plant physiology, N.S., vol. 12A: Physiological plant ecology I. Responses to the physical environment, pp. 57–107, Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H., eds. Springer, Berlin Heidelberg New YorkGoogle Scholar
  6. Blenkinsop, P.G., Dale, J.E. (1974) The effects of shade treatment and light intensity on ribulose-1,5-diphosphate carboxylase activity and fraction I protein level in the first leaf of barley. J. Exp. Bot. 25, 899–912Google Scholar
  7. Boyer, J.S. (1971) Non-stomatal inhibition of photosynthesis in sunflower at low leaf water potentials and high light intensities. Plant Physiol. 48, 532–536Google Scholar
  8. Boyer, J.S. (1976) Water deficiets and photosynthesis. In: Water deficits and plant growth, vol. IV, pp. 153–190, Kozlowski, T.T., ed. Academic Press, New York San FranciscoGoogle Scholar
  9. Bunce, J.A., Patterson, D.T., Peet, M.M., Randall, S.A. (1977) Light acclimation during and after leaf expansion in soybean. Plant Physiol. 60, 255–258Google Scholar
  10. Caemmerer, S. von (1981) On the relationship between chloroplast biochemistry and gas exchange of leaves. Ph.D. thesis, Australian National University, CanberraGoogle Scholar
  11. Caemmerer, S. von, Farquhar, G.D. (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153, 376–387Google Scholar
  12. Carmi, A., Koller, D. (1979) Regulation of photosynthetic activity in the primary leaves of bean (Phaseolus vulgaris L.) by materials moving in the water conducting system. Plant Physiol. 64, 285–288Google Scholar
  13. Collatz, G.J. (1977) Influence of certain environmental factors on photosynthesis and photorespiration in Simmondsia chinensis. Planta 134, 127–132Google Scholar
  14. Downton, W.J.S., Björkman, O., Pike, C. (1980) Consequences of increased atmospheric concentrations of carbon dioxide for growth and photosynthesis of higher plants. In: Carbon dioxide and climate: Australian research, pp. 143–151, Pearman, G.I., ed. Australian Academy of Sciences, CanberraGoogle Scholar
  15. Evans, J.R. (1983) Nitrogen and photosynthesis in the flag leaf of wheat (Triticum aestivum) L. Plant Physiol. 72, 297–302Google Scholar
  16. Farquhar, G.D., Caemmerer, S. von (1981) Electron transport limitations on the CO2 assimilation rate of leaves: a model and some observations in Phaseolus vulgaris L. Proc. Vth Int. Congr. on Photosynthesis, Halkidiki, Greece, vol. IV, pp. 163–175, Akoyunoglou, G., ed. Balaban, PhiladelphiaGoogle Scholar
  17. Farquhar, G.D., Caemmerer, S. von (1982) Modelling of photosynthetic response to environmental conditions. In: Encyclopedia of plant physiology, N.S., vol. 12B: Physiological plant ecology II. Water relations and carbon assimilation, pp. 549–588, Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H., eds. Springer, Berlin Heidelberg New YorkGoogle Scholar
  18. Farquhar, G.D., Caemmerer, S. von, Berry, J.A. (1980) A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta 149, 78–90Google Scholar
  19. Geiger, D.R. (1976) Effects of translocation and assimilate demand on photosynthesis. Can. J. Bot. 54, 2337–2345Google Scholar
  20. Hatch, M.D., Slack, C.R., Bull, T.A. (1969) Light induced changes in the content of some enzymes of the C4-dicarboxylic acid pathway of photosynthesis and its effect on other characteristics of photosynthesis. Phytochemistry 3, 697–706Google Scholar
  21. Hewitt, E.J., Smith, T.A. (1975) Plant mineral nutrition. English University Press, LondonGoogle Scholar
  22. Hodgkinson, K.C. (1974) Influence of partial defoliation on photosynthesis, photorespiration and transpiration by lucerne leaves of different ages. Aust. J. Plant Physiol. 1, 561–578Google Scholar
  23. Hofstra, G., Hesketh, J.D. (1975) The effects of temperature and CO2 enrichment on photosynthesis in soybean. In: Environmental and biological control of photosynthesis, pp. 71–80, Marcelle, R., ed. Junk, The HagueGoogle Scholar
  24. Jenkins, G.I., Woolhouse, H.W. (1981) Photosynthetic electron transport during senescence of the primary leaves of Phaseolus vulgaris L. I. Non-cyclic electron transport. J. Exp. Bot. 32, 467–478Google Scholar
  25. Jones, H.G. (1973) Moderate term water stress and associated changes in some photosynthetic parameters in cotton. New Phytol. 72, 1095–1105Google Scholar
  26. Jordan, D.B., Ogren, W.L. (1981) Species variation in the specificity of ribulose bisphosphate carboxylase/oxygenase. Nature (London) 291, 513–515Google Scholar
  27. Jurik, W.T., Chabot, J.F., Chabot, B.F. (1979) Ontogeny of photosynthetic performance in Fragaria virginiana under changing light regimes. Plant Physiol. 63, 542–547Google Scholar
  28. Kaiser, W.M., Kaiser, G., Prachuab, P.K., Wildman, S.G., Heber, U. (1981) Photosynthesis of isolated chloroplasts and protoplasts under osmotic stress. Inhibition of photosynthesis of intact chloroplasts, protoplasts and leaf slices at low water potentials. Planta 153, 416–422Google Scholar
  29. Keck, R.W., Boyer, J.S. (1974) Chloroplast response to low leaf water potentials. III. Differing inhibition of electron transport and photophosphorylation. Plant Physiol. 53, 474–429Google Scholar
  30. King, R.W., Wardlaw, I.F., Evans, L.T. (1967) Effect of assimilate utilization on photosynthetic rate in wheat. Planta 77, 261–276Google Scholar
  31. Kriedemann, P.E., Sward, R.J., Downton, W.J.S. (1976) Vine response to carbon dioxide enrichment during heat therapy. Aust. J. Plant Physiol. 3, 605–618Google Scholar
  32. Laisk, A., Oya, V. (1971) Changed resistance of aspen mesophyll as a response to rapid leaf drying (in Russian with English summ.). Fiziol. Rast. 18, 553–562Google Scholar
  33. Lorimer, G.H., Badger, M.R., Andrews, T.J. (1977) D-ribulose-1,5-bisphosphate carboxylase-oxygenase: improved methods for the activation and assay of catalytic activities. Anal. Biochem. 78, 66–75Google Scholar
  34. Louwerse, W., van der Zweerde, W. (1977) Photosynthesis, transpiration and leaf morphology of Phaseolus vulgaris and Zea mays grown at different irradiances in artificial and sunlight. Photosynthetica 11, 11–24Google Scholar
  35. Medina, E. (1969) Relationships between nitrogen level, photosynthetic capacity and carboxy-dismutase activity in Atriplex patula leaves. Carnegic Inst. Washington Yearb. 68, 655–662Google Scholar
  36. Moldau, H. (1973) Effects of various water regimes on stomatal and mesophyll conductances of bean leaves. Photosynthetica 7, 1–7Google Scholar
  37. Mooney, H.A., Björkman, O., Collatz, G.J. (1977) Photosynthetic acclimation to temperature and water stress in the desert shrub Larrea divaricata. Carnegie Inst. Washington Yearb. 76, 328–335Google Scholar
  38. Neales, T.F., Treharne, K.J., Wareing, P.F. (1971) A relationship between net photosynthesis, diffusive resistance, and carboxylating enzyme activity in bean leaves. In: Photosynthesis and photorespiration, pp. 89–96, Hatch, M., Osmond, C.B., Slatyer, R.O., eds. Wiley, New YorkGoogle Scholar
  39. Nevins, D.J., Loomis, R.S. (1970) Nitrogen nutrition and photosynthesis in sugar beet (Beta vulgaris L.). Crop Sci. 10, 21–25Google Scholar
  40. O'Toole, J.C., Crookston, R.K., Treharne, K.J., Ozbun, J.L. (1976) Mesophyll resistance and carboxylase activity. Comparison under water stress conditions. Plant Physiol. 57, 465–468Google Scholar
  41. Peet, M.M., Kramer, P.J. (1981) Effects of decreasing source/sink ratio in soybeans on photosynthesis, photorespiration, transpiration and yield. Plant Cell Environ. 3, 201–206Google Scholar
  42. Powles, S.B., Critchley, C. (1980) Effect of light intensity during growth on photoinhibition of intact attached bean leaflets. Plant Physiol. 65, 1181–1187Google Scholar
  43. Radin, J.W., Ackerson, R.C. (1981) Water relations of cotton plants under nitrogen dificiency. III. Stomatal conductance, photosynthesis, and abscisic acid accumulation during drought. Plant Physiol. 67, 115–119Google Scholar
  44. Seemann, J.R., Berry, J.A. (1982) Interspecific differences in the kinetic properties of RuBP carboxylase protein. Carnegie Inst. Washington Yearb. 81, 78–82Google Scholar
  45. Seemann, J.R., Tepperman, J.M., Berry, J.A. (1981) The relationship between photosynthetic performance and the levels and kinetic properties of RuBP carboxylase-oxygenase from desert winter-annuals. Carnegie Inst. Washington Yearb. 80, 67–72Google Scholar
  46. Sharkey, T.D., Badger, M.R. (1982) Effects of waterstress on photosynthetic electron transport, photophosphorylation, and metabolite levels of Xanthium strumarium mesophyll cells. Planta 156, 199–206Google Scholar
  47. Simpson, E. (1978) Biochemical and genetic studies of the synthesis and degradation of RuP2 carboxylase. In: Photosynthetic carbon assimilation, pp. 113–125, Siegelmann, H.W., Hind, G., eds. Plenum Press, New YorkGoogle Scholar
  48. Thorne, J.H., Koller, H.R. (1974) Influence of assimilate demand on photosynthesis, diffusive resistance, translocation, and carbohydrate levels of soybean leaves. Plant Physiol. 54, 201–207Google Scholar
  49. Troughton, J.H., Slatyer, R.O. (1969) Plant water status, leaf temperature and the calculated mesophyll resistance to carbon dioxide of cotton leaves. Aust. J. Biol. Sci. 22, 815–827Google Scholar
  50. Wareing, P.F., Khalifa, M.M., Treharne, K.J. (1968) Rate-limiting processes in photosynthesis at saturating light intensities. Nature (London) 220, 453–457Google Scholar
  51. Wild, A., Rühle, W., Grahl, H. (1975) The effect of light intensity during growth of Sinapis alba on the electron transport and non-cyclic photophosphorylation. In: Environmental and biological control of photosynthesis, pp. 115–121, Marcelle, R., ed. Junk, The HagueGoogle Scholar
  52. Wong, S.C. (1979) Elevated atmospheric partial pressure of CO2 and plant growth. I. Interactions of nitrogen and photosynthetic capacity and C3 and C4 plants. Oecologia 44, 68–74Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. von Caemmerer
    • 1
  • G. D. Farquhar
    • 1
  1. 1.Department of Environmental Biology, Research School of Biological SciencesAustralian National UniversityCanberra CityAustralia

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