Planta

, Volume 160, Issue 4, pp 305–313

The relationship between steady-state gas exchange of bean leaves and the levels of carbon-reduction-cycle intermediates

  • Murray R. Badger
  • Thomas D. Sharkey
  • Susanne von Caemmerer
Article

DOI: 10.1007/BF00393411

Cite this article as:
Badger, M.R., Sharkey, T.D. & von Caemmerer, S. Planta (1984) 160: 305. doi:10.1007/BF00393411

Abstract

The relationship between the gas-exchange characteristics of attached leaves of Phaseolus vulgaris L. and the pool sizes of several carbon-reduction-cycle intermediates was examined. After determining the rate of CO2 assimilation at known intercellular CO2 pressure, O2 pressure and light, the leaf was rapidly killed (<0.1 s) and the levels of ribulose-1,5-bisphosphate (RuBP), 3-phosphoglyceric acid (PGA), fructose-1,6-bisphosphate, fructose-6-phosphate, glucose-6-phosphate, glyceraldehyde-3-phosphate, and dihydroxyacetone phosphate were measured. In 210 mbar O2, photosynthesis appeared RuBP-saturated at low CO2 pressure and RuBP-limited at high CO2 pressure. In 21 mbar (2%) O2, the level of RuBP always appeared saturating. Very high levels of PGA and other phosphate-containing compounds were found with some conditions, especially under low oxygen.

Key words

Phaseolus (gas exchange) Photosynthesis (intermediates) Ribulose-1,5-bisphosphate carboxylase-oxygenase 

Abbreviations and symbols

C1

intercellular CO2 pressure

PGA

3-phosphoglyceric acid

RuBP

ribulose-1,5-bisphosphate

Rubisco

ribulose-1,5-bisphosphate carboxylase-oxygenase

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Murray R. Badger
    • 1
  • Thomas D. Sharkey
    • 1
  • Susanne von Caemmerer
    • 1
  1. 1.Department of Environmental Biology, Research School of Biological SciencesAustralian National UniversityCanberra CityAustralia
  2. 2.Desert Research InstituteRenoUSA

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