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Planta

, Volume 159, Issue 4, pp 373–381 | Cite as

Monoterpene hydrocarbon biosynthesis by isolated leucoplasts of Citrofortunella mitis

  • Michel Gleizes
  • Ginette Pauly
  • Jean-Pierre Carde
  • Anne Marpeau
  • Colette Bernard-Dagan
Article

Abstract

A plastid vesicle preparation isolated from exocarpium of young Citrofortunella mitis (calamondin) fruits was able to synthesise monoterpene hydrocarbons when incubated with isopentenyl pyrophosphate. The electron-microscope comparison between this organelle fraction and the various plastid classes present in the peel tissues has shown the structural identity between these plastid vesicles and the leucoplasts of the epithelial cells lining the secretory pockets. The monoterpene biosynthesis required the presence of dimethylallyl pyrophosphate, Mn2+ or Mg2+ and was increased by addition of 2-mercaptoethanol. Evidence is provided that the leucoplast vesicles act as a complete system in which occur all the successive steps involved in monoterpene hydrocarbon elaboration from isopentenyl pyrophosphate.

Key words

Citrofortunella Leucoplast (isolated) Monoterpene biosynthesis 

Abbreviations

DMAPP

dimethylallyl pyrophosphate

IPP

isopentenyl pyrophosphate

Tricine

N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl] glycine

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Michel Gleizes
    • 1
  • Ginette Pauly
    • 1
  • Jean-Pierre Carde
    • 1
  • Anne Marpeau
    • 1
  • Colette Bernard-Dagan
    • 1
  1. 1.Laboratoire de Physiologie Cellulaire Végétale, E.R.A. au C.N.R.S., no 403Université de Bordeaux ITalence CedexFrance

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