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Planta

, Volume 175, Issue 4, pp 532–538 | Cite as

Production of gibberellins and indole-3-acetic acid by Rhizobium phaseoli in relation to nodulation of Phaseolus vulgaris roots

  • R. Atzorn
  • A. Crozier
  • C. T. Wheeler
  • G. Sandberg
Article

Abstract

Similar ranges of gibberellins (GAs) were detected by high-performance liquid chromatography (HPLC)-immunoassay procedures in ten cultures of wild-type and mutant strains of Rhizobium phaseoli. The major GAs excreted into the culture medium were GA1 and GA4. These identifications were confirmed by combined gas chromatographymass spectrometry. The HPLC-immunoassays also detected smaller amounts of GA9- as well as GA20-like compounds, the latter being present in some but not all cultures. In addition to GAs, all strains excreted indole-3-acetic acid (IAA) but there was no obvious relationship between the amounts of GA and IAA that accumulated. The Rhizobium strains studied included nod and fix mutants, making it unlikely that the IAA- and GA-biosynthesis genes are closely linked to the genes for nodulation and nitrogen fixation.

The HPLC-immunoassay analyses showed also that nodules and non-nodulated roots of Phaseolus vulgaris L. contained similar spectra of GAs to R. phaseoli culture media. The GA pools in roots and nodules were of similar size, indicating that Rhizobium does not make a major contribution to the GA content of the infected tissue.

Key words

Auxin (IAA), production by Rhizobium Gibberellin production by Rhizobium Mutant (RhizobiumNitrogen fixation Phaseolus (nodulation) Rhizobium (mutants) Root nodule 

Abbreviations

EIA

enzyme immunoassay

GAn

gibberellin An

GC-MS

gas chromatography-mass spectrometry

HPLC

high-performance liquid chromatography

IAA

indole-3-acetic acid

Me

methyl ester

RIA

radioimmunoassay

TLC

thin-layer chromatography

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • R. Atzorn
    • 1
  • A. Crozier
    • 1
  • C. T. Wheeler
    • 1
  • G. Sandberg
    • 2
  1. 1.Department of BotanyThe UniversityGlasgowUK
  2. 2.Department of Forest Genetics and Plant PhysiologySwedish University of Agricultural SciencesUmeåSweden

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