Marine Biology

, Volume 84, Issue 2, pp 175–182 | Cite as

Genetic evidence of self-fertilization in the sea anemone Epiactis prolifera

  • A. Bucklin
  • D. Hedgecock
  • C. Hand
Article

Abstract

Biochemical genetic variation provided evidence for the mode of reproduction of brooded young in the sea anemone Epiactis prolifera Verrill, 1869. Individuals of E. prolifera are female when small but hermaphroditic when large (i.e., gynodioecious); juveniles are brooded externally on the column. Brooding individuals collected from 6 intertidal sites (5 in central California and 1 in Washington State, USA) in the spring and summer of 1980 were assayed for gene-enzyme variation by starch-gel electrophoresis. Three of 12 enzyme loci were polymorphic; phosphoglucose isomerase appeared to be encoded by two, closely linked loci. Genotypic frequencies deviated markedly from expected random mating proportions. Only three heterozygotes were found; two were heterozygous at all three polymorphic loci, and the other was polymorphic at the two PGI loci. All 158 juveniles from 25 brooding individuals were assayed (2–19 juveniles per parent). Juveniles on homozygous adults were always identical to their parent. However, brooded young of heterozygous individuals were not identical to their parent. but showed 1:2:1 phenotypic segregation ratios consistent with reproduction by self-fertilization. This genetic evidence together with findings of marked heterozygote deficiencies and genetic identity of homozygous adults and their brooded young supports the conclusion that E. prolifera usually reproduces by self-fertilization, and cross-fertilization is rate.

Keywords

Isomerase Polymorphic Locus Random Mating Segregation Ratio Genetic Evidence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature cited

  1. Allard R. W.: Genetic systems associated with colonizing ability in predominantly self-pollinated species. In: The genetics of colonizing species, pp 405–423. Ed. by H. G. Baker and G. L. Stebbins. New York: Academic Press 1965Google Scholar
  2. Allard R. W.: The mating system and microevolution. Genetics, Princeton 79, 115–126 (1975)Google Scholar
  3. Allard, R. W., S. K. Jain and P. L. Workman: The population genetics of inbreeding species. Adv. Genet. 14, 55–131 (1966)Google Scholar
  4. Antonovics, J.: Evolution in closely adjacent plant populations. V. Evolution of self-fertility. Heredity, Lond. 23, 219–238 (1968)Google Scholar
  5. Avise, J. C. and G. B. Kitto: Phosphoglucose isomerase gene duplication in the bony fishes: an evolutionary history. Biochem. Genet. 8, 113–132 (1973)Google Scholar
  6. Ayala, F. J., D. Hedgecock, G. Zumwalt and J. W. Valentine: Genetic variation in Tridacna maxima, an ecological analog of some unsuccessful evolutionary lineages. Evolution, Lawrence, Kansas 27, 177–191 (1973)Google Scholar
  7. Barnes, H. andD. J. Crisp: Evidence of self-fertilization in certain species of barnacles. J. mar. biol. Ass. U.K. 35, 631–639 (1956)Google Scholar
  8. Beckwitt, R.: Electrophoretic evidence for self-fertilization in 2 species of spirorbid polychaetes. Bull. Sth. Calif. Acad. Sci. 81, 61–68 (1982)Google Scholar
  9. Black, R. and M. S. Johnson: Asexual viviparity and population genetics of Actinia tenebrosa. Mar. Biol. 53, 27–31 (1979)Google Scholar
  10. Brown, A. H. D.: Isozymes, plant population structure and genetic conservation. Theor. appl. Genet. 52, 145–157 (1978)Google Scholar
  11. Brown, A. H. D.: Enzyme polymorphism in plant populations. Theor. Popul. Biol. 15, 1–42 (1979)Google Scholar
  12. Bucklin, A. and D. Hedgecock: Biochemical genetic evidence for a third species of Metridium (Coelenterata: Actiniaria). Mar. Biol. 66, 1–7 (1982)Google Scholar
  13. Carlgren, O.: A survey of the Ptychodactiaria, Corallimorpharia and Actiniaria. K. svenska VetenskAkad. Handl. 1, 1–121 (1949)Google Scholar
  14. Carter, M. A. and M. E. Funnell: Reproduction and brooding in Actinia. In: Developmental and cellular biology of coelenterates, pp 17–22. Ed. by P. Tardent and R. Tardent. New York: Elsevier 1980Google Scholar
  15. Carter, M. A. and C. H. Thorp: The reproduction of Actinia equina L. var. mesembryanthenum. J. mar. biol. Ass. U.K. 59, 989–1001 (1979)Google Scholar
  16. Carter, M. A. and J. P. Thorpe: Ecological and genetic evidence that Actinia equina var. mesembryanthemum and var. fragacea are not conspecific. J. mar. biol. Ass. U.K. 61, 79–93 (1981)Google Scholar
  17. Charlesworth, B. and D. Charlesworth: A model for the evolution of dioecy and gynodioecy. Am. Nat. 112, 975–997 (1978)Google Scholar
  18. Chia, F.-S.: Sea anemone reproduction: patterns and adaptive radiations. In: Coelenterate ecology and behavior, pp 261–270. Ed. by G. O. Mackie. New York: Plenum Press 1976Google Scholar
  19. Clark, W. C.: Hermaphroditism as a reproductive strategy for metazoans: some correlated benefits. N. Z. Jl Zool. 5, 769–780 (1978)Google Scholar
  20. Davies, W. E. and N. R. Young: Self-fertility in Trifolium fragiferum. Heredity, Lond. 21, 615–624 (1966)Google Scholar
  21. Dunn, D. F.: The natural history of the sea anemone Epiactis prolifera Verrill 1869 with special reference to its reproductive biology, 187 pp. Ph. D. dissertation, University of California, Berkeley 1972Google Scholar
  22. Dunn, D. F.: Gynodioecy in an animal. Nature, Lond. 253, 528–529 (1975a)Google Scholar
  23. Dunn, D. F.: Reproduction of the externally brooding sea anemone Epiactis prolifera Verrill, 1869. Biol. Bull. mar. biol. Lab., Woods Hole 148, 199–218 (1975b)Google Scholar
  24. Dunn, D. F.: Dynamics of external brooding in the sea anemone Epiactis prolifera. Mar. Biol. 39, 41–49 (1977)Google Scholar
  25. Finnerty, V. and G. Johnson: Post-translational modification as a potential explanation of high levels of enzyme polymorphism: xanthine dehydrogenase and aldehyde oxidase in Drosophila melanogaster. Genetics, Princeton 91, 692–722 (1979)Google Scholar
  26. Gashout, S. E. and R. F. G. Ormond: Evidence for parthenogenetic reproduction in the sea anemone Actinia equina L. J. mar. biol. Ass. U.K. 59, 975–987 (1979)Google Scholar
  27. Ghiselin, M. T.: The evolution of hermaphroditism among animals. Q. Rev. Biol. 44, 189–208 (1969)Google Scholar
  28. Ghiselin, M. T.: The economy of nature and the evolution of sex, 346 pp. Berkeley and Los Angeles: University of California Press 1974Google Scholar
  29. Gottlieb, L. D.: Evidence for duplication and divergence of the structural gene for phosphoglucoisomerase in diploid species of Clarkia. Genetics, Princeton 86, 289–307 (1977)Google Scholar
  30. Gottlieb, L. D. and N. F. Weeden: Gene duplication and phylogeny in Clarkia. Evolution, Lawrence, Kansas 33, 1024–1039 (1979)Google Scholar
  31. Hand, C.: The sea anemones of central California, Part II, The Endomyarian and Mesomyarian anemones. Wasmann J. Biol. 13, 189–251 (1955)Google Scholar
  32. Hedrick, P.: Genetics of populations, 629 pp. Boston: Science Books International 1983Google Scholar
  33. Hoffman, R. J.: Genetics and asexual reproduction of the sea anemone Metridium senile. Biol. Bull. mar. biol. Lab., Woods Hole 151, 478–488 (1976)Google Scholar
  34. Jain, S. K. and R. W. Allard: The effects of linkage, epistasis and inbreeding on population changes under selection. Genetics, Princeton 53, 633–659 (1966)Google Scholar
  35. Larkman, A. U. and M. A. Carter: The spermatozoon of Actinia equina L. var. mesembryanthemum. J. mar. biol. Ass. U.K. 60, 193–204 (1980)Google Scholar
  36. Levene, H.: On a matching problem arising in genetics. Ann. math. Statist. 20, 91–94 (1949)Google Scholar
  37. Lewontin, R. C.: The interaction of selection and linkage. I. General considerations; heterotic models. Genetics, Princeton 49, 49–67 (1964)Google Scholar
  38. Moore, D. M. and H. Lewis: The evolution of self-pollination in Clarkia xantiana. Evolution, Lawrence, Kansas 19, 104–114 (1965)Google Scholar
  39. Orr, J., J. P. Thorpe and M. A. Carter: Biochemical genetic information of the asexual reproduction of brooded offspring in the sea anemone Actinia equina. Mar. Ecol. Prog. Ser. 7, 227–229 (1982)Google Scholar
  40. Ruth, R. C. and F. Wold: The subunit structure of glycolytic enzymes. Comp. Biochem. Physiol. 54B, 1–6 (1976)Google Scholar
  41. Shick, J. M. and A. N. Lamb: Asexual reproduction and genetic population structure in the colonizing sea anemone Haliplanella luciae. Biol. Bull. mar. biol. Lab.,Woods Hole 153, 604–617 (1977)Google Scholar
  42. Sokal, R. R. and F. J. Rohlf: Biometry. The principles and practice of statistics in biological research, 2nd ed. 859 pp. San Francisco: W. H. Freeman & Co. 1981Google Scholar
  43. Stebbins, G. L.: Variation and evolution in plants, 643 pp. New York: Columbia University Press 1950Google Scholar
  44. Tracey, M. L., K. Nelson, D. Hedgecock, R. A. Shleser and M. L. Pressick: Biochemical genetics of lobsters: genetic variation and the structure of American lobster (Homarus americanus) populations. J. Fish. Res. Bd Can. 32, 2091–2101 (1975)Google Scholar
  45. Uchida, T.: A brood-caring actinian subject to a wide range of color variation. J. Fac.Sci. Hokkaido imp. Univ. (VI, Zool.) 3, 17–31 (1934)Google Scholar
  46. Valentine, J. W.: Genetic strategies of adaptation. In: Molecular evolution, pp 78–94. Ed. by F. J. Ayala. Sunderland, Mass.: Sinauer Associates, Inc. 1976Google Scholar
  47. Wright, S.: The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution, Lawrence, Kansas 19, 395–420 (1965)Google Scholar
  48. Wright, S.: Evolution and the genetics of populations. Vol. 2. The theory of gene frequencies, 511 pp. Chicago: University of Chicago Press 1969Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • A. Bucklin
    • 1
  • D. Hedgecock
    • 1
  • C. Hand
    • 1
  1. 1.Bodega Marine LaboratoryUniversity of CaliforniaBodega BayUSA

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