Marine Biology

, Volume 89, Issue 1, pp 9–20 | Cite as

Adaptation to low photon flux densities in Protogonyaulax tamarensis var. excavata, with reference to chloroplast photomorphogenesis

  • C. M. Yentsch
  • T. L. Cucci
  • D. A. Phinney
  • R. Selvin
  • H. E. Glover
Article
Accepted:

Abstract

Changes in cellular chlorophyll content, cell volume, and light scatter of a New England red tide dinoflagellate, Protogonyaulax tamarensis var. excavata (clone GT-429), cultured in various light regimes are reported. Individual cells were analyzed, using flow cytometry and compared to traditional bulk measurements. Compared to high photon flux densities (182 μEin m-2 s-1), changes were measured that reflected increased chlorophyll fluorescence and increased cell volume at reducec photon flux densities when cell division was sustained, and increased flourescence and decreased cell volume when cell division ceased. These optical changes were accompanied by conformational changes in the chloroplasts. We found no change in photosynthetic carboxylating enzyme activities. We suggest that this photomorphogenesis of the chloroplasts at low photon flux densities may be an indication of stress and survival vs adaptive value to these persistent cells.

Keywords

Chlorophyll Cell Volume Chlorophyll Content Dinoflagellate Chlorophyll Fluorescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Literature cited

  1. Brand, L. E., R. R. L. Guillard and L. S. Murphy: A method for the rapid and precise determination of acclimated phytoplankton reproduction rates. J. Plank. Res. 3, 193–201 (1981)Google Scholar
  2. Brown, T. E. and F. L. Richardson: The effect of growth evironment on the physiology of algae: light intensity. J. Phycol. 4, 38–54 (1968)Google Scholar
  3. de Duve, C.: Microbodies in the living cell. Sci. Am. 248, 74–84 (1983)Google Scholar
  4. de Duve, C. and P. Baudhuin: Peroxisomes (microbodies and related particles). Physiol. Rev. 46, 323–357 (1966)Google Scholar
  5. Dring, M. J.: Chromatic adaptation of photosynthesis in benthic marine algae: an examination of its ecological significance using a theoretical model. Limnol. Oceanogr. 26, 271–284 (1981)Google Scholar
  6. Falkowski, P. G.: Light-shade adaption in marine phytoplankton. In: Primary productivity in the sea, pp 99–119. Ed. by P. G. Falkowski New York: Plenum Press 1980Google Scholar
  7. Falkowski, P. G. and T. G. Owens: Light-shade adaptation. Two strategies in marine phytoplankters. Plant Physiol. 66, 592–595 (1980)Google Scholar
  8. Glover, H. E. and I. Morris: Photosynthetic carboxylating enzymes in marine phytoplankton. Limnol. Oceanogr. 24, 510–519 (1979)Google Scholar
  9. Guillard, R. R. L. and J. Ryther: Studies on marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervacea (cleve) Gran. Can. J. Microbiol. 8, 229–239 (1962)Google Scholar
  10. Holligan, P. M. and D. S. Harbour: The vertical distribution and succession of phytoplankton in the Western English Channel in 1975 and 1976. J. mar. Biol. Ass. U.K. 57, 1075–1093 (1976)Google Scholar
  11. Holligan, P. M., W. M. Balch and C. M. Yentsch: Significance of subsurface chlorophyll, nitrite and ammonium maximum in relation to nitrogen for phytoplankton growth in stratified waters of the Gulf of Maine. J. mar. Res. 42, 1051–1073 (1984)Google Scholar
  12. Kiefer, D. A.: Chlorophyll a fluorescence in marine centric diatoms: responses of chloroplasts to light and nutrient stress. Mar. Biol. 23, 39–46 (1973)Google Scholar
  13. Ley, A. C. and D. C. Mauzerall: Absolute absorption cross-sections for photosystem II and the minimum quantum requirement for photosynthesis in Chlorella vulgaris. Biochim. biophys. Acta 680, 91–106 (1982)Google Scholar
  14. Loftus, M. E. and H. H. Seliger: Some limitations of the in vivo fluorescence technique. Chesapeake Sci. 16, 79–92 (1975)Google Scholar
  15. Owens, O. V. H. and H. H. Seliger: Effect of light intensity on dinoflagellate growth and photosynthesis. In: Physiological adaptations to the environment, pp 355–364. Ed. by F. J. Vernberg, New York: Intext Educational Publishers 1975Google Scholar
  16. Perry, M. J., M. C. Larsen and R. S. Alberte: Photoadaptation in marine phytoplankton: responses of the photosynthetic unit. Mar. Biol. 62, 91–101 (1981)Google Scholar
  17. Prézelin, B. B.: Light reactions in photosynthesis. In: Physiological bases of phytoplankton ecology, pp 1–46. Ed. by T. Platt. Can. Bull. Fish. aquat. Sci. 210, Ottawa (1981)Google Scholar
  18. Prézelin, B. B. and H. A. Matlick: Time-course of photoadaptation in the photosynthesis-irradiance relationship of a dinoflagellate exhibiting photosynthetic periodicity. Mar. Biol. 58, 85–96 (1980)Google Scholar
  19. Prézelin, B. B. and B. M. Sweeney: Characterization of photosynthetic rhythms in marine dinoflagellates. II. Photosynthesis-irradiance curves and in vivo chlorophyll a fluorescence. Plant Physiol. 60, 384–387 (1977)Google Scholar
  20. Prézelin, B. B. and B. M. Sweeney: Photoadaptation of photosynthesis in two bloom-forming dinoflagellates. In: Toxic dinoflagellate blooms, pp 101–106. Ed. by D. Taylor and H. H. Seliger. New York: Elsevier North Holland 1979Google Scholar
  21. Richardson, K., J. Beardall and J. A. Raven. Adaptation of unicellular algae to irradiance: an analysis of strategies. New Phytol. 93, 157–191 (1983)Google Scholar
  22. Rivkin, R. B., H. H. Seliger, E. Swift and W. H. Biggley: Lightshade adaptation by the oceanic dinoflagellate Pyrocystis noctiluca and P. fusiformis. Mar. Biol. 68, 181–192 (1982)Google Scholar
  23. Sakshaug, E. and O. Holm-Hansen: Factors governing pelagic production in polar oceans. In: Symposium Volume of European Society of Physiologists and Biochemists, (Taormina, Sicily, Sept. 1983). Berlin: Springer-Verlag 1984Google Scholar
  24. Schmitter, R. E. and A. J. Jurkiewicz: Acid phosphatase localization in PAS-bodies of Gonyaulax. J. Cell. Sci. 51, 15–23 (1981)Google Scholar
  25. Spinrad, R. W. and C. M. Yentsch: The optical state of marine photoplankton. (Submitted)Google Scholar
  26. Steidinger, K. A. and E. R. Cox: Free-living dinoflagellates, pp 407–432. Ed. by E. R. Cox. New York: Elsevier North Holland 1980Google Scholar
  27. Tolbert, N. E.: Microbodies — peroxisomes and glyoxysomes. Biochem. Plants 1, 359–388 (1980)Google Scholar
  28. Tyler, M. A. and H. H. Seliger: Annual subsurface transport of a red tide dinoflagellate to its bloom area: water circulation patterns and organism distributions in the Chesapeake Bay. Limnol. Oceanogr. 23, 227–246 (1978)Google Scholar
  29. Tzagoloff, A.: Mitochondria, pp 20–23. New York: Plenum Press 1982Google Scholar
  30. Yentsch, C. M., C. Lewis and C. S. Yentsch: Biological resting in the dinoflagellate Gonyaulax excavata. Bioscience 39, 251–254 1980Google Scholar
  31. Yentsch, C. M., P. K. Horan, K. Muirhead, Q. Dortch, E. Haugen, L. Legendre, L. S. Murphy, M. J. Perry, D. A. Phinney, S. A. Pomponi, R. W. Spinrad, M. Wood, C. S. Yentsch and B. J. Zahuranec: Flow cytometry and cell sorting: a powerful technique for analysis and sorting of aquatic particles. Limnol. Oceanogr. 28, 1275–1280 (1983)Google Scholar
  32. Yentsch, C. S. and D. W. Menzel: A method for the determination of phytoplankton chlorophyll and phaeophytin by flourescence. Deep-Sea Res. 10, 221–231 (1963)Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • C. M. Yentsch
    • 1
  • T. L. Cucci
    • 1
  • D. A. Phinney
    • 1
  • R. Selvin
    • 1
  • H. E. Glover
    • 1
  1. 1.Bigelow Laboratory for Ocean SciencesWest Boothbay HarborUSA

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