Marine Biology

, Volume 80, Issue 3, pp 335–339 | Cite as

Effects of temperature, nutritional factors and salinity on the uptake of L-methionine by the Sydney rock oyster Saccostrea commercialis

  • J. A. Nell
  • P. R. Dunkley


The effects of physiological and nutritional factors and of temperature on the uptake of L-methionine by Saccostrea commercialis were investigated on cultured rock oysters from Port Stephens, New South Wales, Australia, in 1983. Optimum conditions for L-methionine accumulation were 30°C, 18 h exposure and concentrations of other amino acids less than that of L-methionine. The uptake of L-glycine was inhibited by L-methionine in a reciprocal manner. There was no effect of salinity on the accumulation of L-methionine by acclimated oysters. During the latter investigations the range of osmoconformity was found to be 15 to 45‰ S. Oysters take 2 d to conform to new media osmolarities after salinity changes of 15‰. Amino acid supplements in oyster diets should be kept within the same order of magnitude to reduce inhibition of uptake.


Optimum Condition Acid Supplement Nutritional Factor Salinity Change Amino Acid Supplement 
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Literature cited

  1. Anderson, J. W. and W. B. Bedford: The physiological response of the estuarine clam Rangia cuneata (Gray) to salinity. II. Uptake of glycine. Biol. Bull. mar. biol. Lab., Woods Hole 144, 229–247 (1973)Google Scholar
  2. Anderson, R. D. and J. W. Anderson: Effects of salinity and selected petroleum hydrocarbons on the osmotic and chloride regulation of the American oyster, Crassostrea virginica. Physiol. Zoöl. 48, 420–430 (1975)Google Scholar
  3. Bamford, D. R. and R. McCrea: Active absorption of neutral and basic amino acids by the gill of the common cockle, Cerastoderma edule. Comp. Biochem. Physiol. 50A, 811–817 (1975)Google Scholar
  4. Bedford, W. B. and J. W. Anderson: The physiological response of the estuarine clam Rangia cuneata (Gray) to salinity. I. Osmoregulation. Physiol. Zoöl. 45, 255–260 (1972)Google Scholar
  5. Buxton, C. D., R. C. Newell and J. G. Field: Response-surface analysis of the combined effects of exposure and acclimation temperatures on filtration, oxygen consumption and scope for growth in the oyster Ostrea edulis. Mar. Ecol. Prog. Ser. 6, 73–82 (1981)Google Scholar
  6. Chanley, P. E.: Survival of some juvenile bivalves in water of low salinity. Proc. natn. Shellfish. Ass. 48, 52–62 (1957)Google Scholar
  7. Elliott, A. J.: Laboratory investigations into the absorption of dissolved free amino acids by the gill of the mussel Mytilus edulis L. Ir. Fish. Invest. (Ser. B., Mar.) 22, 1–15 (1979)Google Scholar
  8. Gilles, R.: Osmoregulation in three molluscs: Acanthochitona discrepans (Brown), Glycymeris glycymeris (L.) and Mytilus edulis (L.). Biol. Bull. mar. biol. Lab., Woods Hole 142, 25–35 (1972)Google Scholar
  9. Harrison, C.: The essential amino acids of Mytilus californianus. Veliger 18, 189–193 (1976)Google Scholar
  10. Hoyaux, R., R. Gilles and Ch. Jeuniaux: Osmoregulation in molluscs of the intertidal zone. Comp. Biochem. Physiol. 53A, 361–365 (1976)Google Scholar
  11. King, M. G.: Cultivation of the Pacific oyster, (Crassostrea gigas) in a non-tidal hypersaline pond. Aquaculture, Amsterdam 11, 123–136 (1977)Google Scholar
  12. Nell, J. A., M. E. Skeel and P. Dunkley: Uptake of some dissolved organic nutrients by the Sydney rock oyster Saccostrea commercialis. Mar. Biol. 74, 313–318 (1983)Google Scholar
  13. Nell, J. A. and B. Wisely: Experimental feeding of Sydney rock oysters (Saccostrea commercialis) II. Protein supplementation of artificial diets for adult oysters. Aquaculture, Amsterdam 32, 1–9 (1983)Google Scholar
  14. Nell, J. A. and B. Wisely: Experimental feeding of Sydney rock oysters (Saccostrea commercialis). III. Food concentration and fattening procedures. Aquaculture, Amsterdam 37, 197–208 (1984)Google Scholar
  15. Péquignant, E.: A kinetic and autoradiographic study of the direct assimilation of amino acids and glucose by organs of the mussel Mytilus edulis. Mar. Biol. 19, 227–244 (1973)Google Scholar
  16. Pierce, S. K.: The water balance of Modiolus (Mollusca: Bivalvia: Mytilidae): osmotic concentrations in changing salinities. Comp. Biochem. Physiol. 36, 521–533 (1970)Google Scholar
  17. Pierce, S. K.: Volume regulation and valve movements by marine mussels. Comp. Biochem. Physiol. 39A, 103–117 (1971)Google Scholar
  18. Powell, E. N., M. Kasschau, E. Chen, M. Koenig and J. Pecon: Changes in the free amino acid pool during environmental stress in the gill tissue of the oyster, Crassostrea virginica. Comp. Biochem. Physiol. 71A, 591–598 (1982)Google Scholar
  19. Shumway, S. E.: The effect of fluctuating salinity on the tissue water content of eight species of bivalve molluscs. J. comp. Physiol. 116 269–285 (1977)Google Scholar
  20. Shumway, S. E. and R. K. Koehn: Oxygen consumption in the American oyster Crassostrea virginica. Mar. Ecol. Prog. Ser. 9, 59–68 (1982)Google Scholar
  21. Souness, R. A. and G. H. Fleet: Depuration of the Sydney rock oyster, Crassostrea commercialis. Fd Technol. Aust. 31, 397–404 (1979)Google Scholar
  22. Steel, G. D. and J. H. Torrie: Principles and procedures of statistics, 481 pp. New York: McGraw-Hill 1960Google Scholar
  23. Wright, S. H., T. L. Johnson and J. H. Crowe: Transport of amino acids by isolated gills of the mussel Mytilus californianus Conrad. J. exp. Biol. 62, 313–325 (1975)Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • J. A. Nell
    • 1
  • P. R. Dunkley
    • 2
  1. 1.Department of Agriculture New South Wales, Division of FisheriesBrackish Water Fish Culture Research StationSalamander BayAustralia
  2. 2.Medical FacultyUniversity of NewcastleNew South WalesAustralia

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